Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

Yang, Wen‐Bin

doi:10.5772/31649

Cited by 2 publications

(3 citation statements)

References 98 publications

(111 reference statements)

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“…Loss of m/z 162 were noted. This is consistent with glycans with repeating units of β-(1→3) linkage specifically of glucan (Hung 2008;Yang 2012;Chan and Tang 2003). The high mass resolution of some peaks were calculated as (162.1424) n + 22.998977 Da or (162.1424) n + 22.998977 + 18.01524 (mass of reducing end residue) Da where n is the number of hexose units.…”

Section: Figure 2 Elution Curve Of W1p-1 On Sephadex G-100 Column (Wsupporting

confidence: 84%

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Partial Characterization of Glycans from Daedalea quercina (L) Fr.

Clerigo

Yang

Paano

2016

KIMIKA

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Heteroglycans were extracted and isolated from the fruiting bodies of wild mushroom Daedalea quercina (L) Fr. These were obtained from sequential extraction with hot water, 1% Ammonium oxalate then 5% Sodium hydroxide solution followed by ethanol or acidic precipitation affording crude extracts which were further purified by dialysis membrane (MWCO 12 400). Separation by charge and size were done using DEAE-cellulose column and Sephadex G-100 or Sepharose CL-4B. This resulted to water soluble neutral (W1P-1A) and acidic (W1P-2A) glycan isolates, ammonium oxalate soluble (A1P-1) and two alkali soluble (N1P-1 and N1P-2) glycan isolates. Characterization of the hydrolyzed isolates using HPAEC-PAD and MALDI TOF MS showed glucan-rich heteroglycans (W1P-1A and W1P-2A); glucan and mannan rich heteroglycan, (N1P-1) and galactan-rich heteroglycan (A1P-1). The latter being the first reported galactan-rich glycan found in ammonium oxalate mushroom extract. The hydrolysates had approximately 4-5 degree of polymerization with molecular weights ranging from 689.680 to 851.788 Da. The hydrolysate of isolate N1P-2 is a glucan-rich heteroglycan which also contain N-acetyl glucosamine units. It is composed of polymers with 4-5 hexose units as well as N-acetyl glucosamine containing polymers of 6-8 hexose units with molecular weight range of 689.799 to 851.915 Da and 1055.718 to 1379.584 Da, respectively.

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Section: Figure 2 Elution Curve Of W1p-1 On Sephadex G-100 Column (Wsupporting

confidence: 84%

“…The MS data of the five fractions which showed a series of m/z 162.1 Da differences between line spectrum peaks is indicative of the presence of a β-(1 3) monosaccharide linkages among monosaccharide units (Hung 2008;Yang 2012;Chan and Tang 2003).…”

Section: Discussionmentioning

confidence: 94%

Partial Characterization of Glycans from Daedalea quercina (L) Fr.

Clerigo

Yang

Paano

2016

KIMIKA

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“…Analysis of carbohydrates (N-and O-linked) on biomedically important glycoproteins 284 (Yamada & Kakehi, 2011) Tandem mass spectrometry of tagged and permethylated polysaccharides 97 (Yang, 2012) Solid-phase glycan isolation for glycomics analysis 108 (Yang & Zhang, 2012a) Hydrophilic interaction liquid chromatography for structural glycomics 60 (Zauner et al, 2011) Recent progress in quantitative glycoproteomics 39 (Zhang et al, 2012i) Structural characterization of carbohydrates by Fourier transform tandem mass spectrometry 146 2011)…”

Section: Bl Glucanmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

Cited by 2 publications

References 98 publications

Partial Characterization of Glycans from Daedalea quercina (L) Fr.

Partial Characterization of Glycans from Daedalea quercina (L) Fr.

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

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