Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-Alpha GST, rGSTA6*, in rat testis

Hsieh, Cheng-Hsilin; Tsai, Shu-Ping; Yeh, H. C.; Sheu, Tsuey-Chyi; Tam, Ming F.

doi:10.1042/bj3230503

Cited by 21 publications

(21 citation statements)

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“…Indeed, different GST isoenzymes have been identified in the male gonad. Specifically, GST␣ [13][14][15] and GST [16] have been identified in testicular somatic cells and particularly in Leydig and Sertoli cells. GST was shown to be expressed in somatic and germ cells [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 98%

Glutathione S-Transferase Alpha Expressed in Porcine Sertoli Cells Is under the Control of Follicle-Stimulating Hormone and Testosterone1

Benbrahim‐Tallaa

Tabone

Tosser‐Klopp

et al. 2002

Biology of Reproduction

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Glutathione S-transferases (GSTs) are a family of detoxification isoenzymes present in different tissues including the testis and that conjugate many toxic substrates to glutathione. Among these substrates are carcinogens, mutagens and products of oxidative processes. In the present report we show that GSTalpha is expressed in somatic testicular Leydig cells and Sertoli cells. GSTalpha expression in Sertoli cells is under the hormonal control of FSH, testosterone, and estradiol. In Leydig cells, immunoreactive GSTalpha was present at the neonatal, pubertal, and adult periods. In Sertoli cells, GSTalpha was predominant in pubertal and adult testes (but not in neonatal testes), suggesting that its expression is controlled by gonadotropins. The regulatory action and the mechanisms of action of FSH and testosterone on GSTalpha mRNA and protein levels were studied by using a model of primary cultures of porcine testicular Sertoli cells. FSH increased GSTalpha mRNA levels in a dose-dependent manner (ED50 = 18.5 nm/ml) with a maximal effect observed after 48 h of exposure (a 3-fold increase; P < 0.001). In addition, FSH increased GSTalpha protein, which was detected as a doublet of 28 kDa. Treatment with testosterone enhanced GSTalpha mRNA levels in a dose-dependent (ED50 = 1.4 ng/ml) and time-dependent manner with a maximal effect delayed at 8 h of exposure (a 2-fold increase; P < 0.001). Similarly, Sertoli cell treatment with testosterone metabolites, dihydrotestosterone (DHT) and estradiol, led to an increase in GSTalpha mRNA levels. Because stimulatory effects of FSH and androgens were also observed on GSTalpha protein, we therefore had to determine whether the different hormones were affecting GSTalpha gene transcriptional activity, or GSTalpha mRNA stability, or both. FSH and 8-Br-cAMP (but not testosterone) increased the stability of GSTalpha mRNA. The effects of FSH and testosterone on GSTalpha protein were additive, confirming that both hormones act through distinct mechanisms on the expression of the enzyme. Taken together, the present observations indicate that Sertoli cell GSTalpha is targeted by FSH, testosterone, and its metabolites, and they reinforce the concept that Sertoli cells exert a protective role and are under endocrine control to ward against toxic agents in the context of Sertoli-germ cell interactions during spermatogenesis.

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Section: Introductionmentioning

confidence: 98%

Glutathione S-Transferase Alpha Expressed in Porcine Sertoli Cells Is under the Control of Follicle-Stimulating Hormone and Testosterone1

Benbrahim‐Tallaa

Tabone

Tosser‐Klopp

et al. 2002

Biology of Reproduction

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“…In contrast, in the testicular cytosol, almost 50% of the transferase activities is due to isoenzymes with acidic (less than pI 7.0) pI values. In rat testis, rGSTA3-3, rGSTM2-2 and rGSTM6-6 are the major isoenzymes (20,22,23). Besides these isoenzymes, rGSTPI-I (24), rGSTA4-4 (25), rGSTM3-3, rGSTM5-5 (26) and rGSTT2-2 occur in this tissue (20,23,26).…”

Section: Resultsmentioning

confidence: 99%

“…In rat testis, rGSTA3-3, rGSTM2-2 and rGSTM6-6 are the major isoenzymes (20,22,23). Besides these isoenzymes, rGSTPI-I (24), rGSTA4-4 (25), rGSTM3-3, rGSTM5-5 (26) and rGSTT2-2 occur in this tissue (20,23,26). In contrast to the single microsomal GST found in rat liver, at least three GST isozymes were identified in testis microsomes (27).…”

Section: Resultsmentioning

confidence: 99%

Effects of dietary broccoli on rat testicular xenobiotic metabolizing enzymes

Vang

Mehrota

Georgellis

et al. 1999

Eur. J. Drug Metab. Pharmacokinet.

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The effects of vegetables on the activities of various metabolizing enzymes in liver and intestine have been studied intensively, whereas studies on effects on testicular metabolizing enzymes are lacking. The present report is the first describing the effects of dietary broccoli on the activities of a number of xenobiotic metabolizing enzymes from rat testes. Groups of male Wistar rats were fed a semisynthetic diet with 10% (w/w) freeze-dried broccoli for 1 week. Different broccoli samples with varying content of glucosinolates were used. Dietary broccoli significantly increased the activities of two testicular phase II enzymes--glutathione S-transferase (1.6-fold) and UDP-glucuronosyl transferase (1.8-fold). The activities of these enzymes differed significantly depending on the conditions during cultivation of the broccoli, because of differences in the content of glucosinolates and other secondary plant metabolites. The levels of two glutathione S-transferase subunits, rGSTM2 and rGSTA, were determined using Western blotting analysis and the levels of both subunits were reduced in animals fed broccoli grown at low S-fertilizer level. Broccoli did not statistically significantly modulate the activities of the phase I enzymes, epoxide hydrolase or NAD(P)H quinone-oxidoreductase, or the phase II enzyme p-sulphotransferase, or the anti-oxidative enzymes catalase and total glutathione peroxidase in rat testes. In general, dietary broccoli affects phase I and phase II enzyme levels in rat testes much less than found in liver, however, two rat testicular phase II xenobiotic metabolizing enzymes were induced.

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“…It was postulated that ABP is required for maintaining high levels of testosterone inside the tubular compartment, since there is a lack of storage capacity inside the ST [5]. There is some evidence that GST isoforms are present in STF, and are actively synthesized and secreted by Sertoli cells as the prime secretors [7,12,21]. It is possible, because of special functional requirements in the testis, that a significant amount of GST enzymes are routed through the secretory pathway in the testicular cells, perhaps in a similar manner as liver GSTs are secreted in the bile [11].…”

Section: Discussionmentioning

confidence: 99%

Relationships Between Levels of Estradiol and Testosterone in Seminal Plasma andGSTM1Polymorphism in Infertile Men

Onaran

Aydemir

Kiziler

et al. 2007

Archives of Andrology

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Glutathione S-transferase M1 (GSTM1) enzyme serves as a steroid-binding protein by its ability to bind to testosterone and estradiol. The levels of total estradiol and testosterone were measured by using an electrochemiluminescence immunoassay in serum and seminal plasma from 103 subjects including 62 subfertile patients. GSTM1 polymorphism was examined using polymerase chain reaction. The estradiol and testosterone levels in seminal plasma were not different in control and subfertile subjects. No role for GSTM1 enzyme as a steroid-binding protein seemed likely as there was also no significant difference in seminal plasma estradiol and testosterone levels according to GSTM1 genotype. Significant positive correlations were found between seminal estradiol and serum estradiol in infertile males, and between seminal testosterone and serum testosterone in fertile males, independent of GSTM1 genotype. GSTM1 polymorphism is not a genetic risk factor of seminal estradiol and testosterone levels in infertile males although further studies are warranted.

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Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-Alpha GST, rGSTA6*, in rat testis

Cited by 21 publications

References 50 publications

Glutathione S-Transferase Alpha Expressed in Porcine Sertoli Cells Is under the Control of Follicle-Stimulating Hormone and Testosterone1

Glutathione S-Transferase Alpha Expressed in Porcine Sertoli Cells Is under the Control of Follicle-Stimulating Hormone and Testosterone1

Effects of dietary broccoli on rat testicular xenobiotic metabolizing enzymes

Relationships Between Levels of Estradiol and Testosterone in Seminal Plasma andGSTM1Polymorphism in Infertile Men

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