Relationships Between Levels of Estradiol and Testosterone in Seminal Plasma and<i>GSTM1</i>Polymorphism in Infertile Men

Onaran, İlhan; Aydemir, Birsen; Kiziler, Ali Rıza; Demiryurek, Tuncay; Alıcı, Bülent

doi:10.1080/01485010600889134

Cited by 11 publications

(4 citation statements)

References 28 publications

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“…In stallions, seminal plasma CRISP3 was positively related to first-cycle conception rate and may be a good marker of fertility [1]. However, male infertility in Nigerians is characterized by raised seminal plasma testosterone [56], and there was no difference in testosterone levels in seminal plasma in fertile and subfertile men [57]. In this study, serum analysis showed higher GPx activity and elevated concentrations of sPLA 2 , CRISP3, and testosterone and lower levels of MDA, IGF-1, and clusterin in the fertile dromedaries in comparison with the infertile group.…”

Section: Biomarkersmentioning

confidence: 99%

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015

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Section: Biomarkersmentioning

confidence: 99%

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015

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“…GSTM proteins are steroid binding proteins characterized by their properties to bind testosterone and estradiol [53]. Upregulation of these antioxidant proteins has been related to drug insult, and detoxification of xenobiotics and oxidative products [54].…”

Section: Discussionmentioning

confidence: 99%

Differential protein expression profile in the hypothalamic GT1-7 cell line after exposure to anabolic androgenic steroids

et al. 2017

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The abuse of anabolic androgenic steroids (AAS) has been considered a major public health problem during decades. Supraphysiological doses of AAS may lead to a variety of neuroendocrine problems. Precisely, the hypothalamic-pituitary-gonadal (HPG) axis is one of the body systems that is mainly influenced by steroidal hormones. Fluctuations of the hormonal milieu result in alterations of reproductive function, which are made through changes in hypothalamic neurons expressing gonadotropin-releasing hormone (GnRH). In fact, previous studies have shown that AAS modulate the activity of these neurons through steroid-sensitive afferents. To increase knowledge about the cellular mechanisms induced by AAS in GnRH neurons, we performed proteomic analyses of the murine hypothalamic GT1-7 cell line after exposure to 17α-methyltestosterone (17α-meT; 1 μM). These cells represent a good model for studying regulatory processes because they exhibit the typical characteristics of GnRH neurons, and respond to compounds that modulate GnRH in vivo. Two-dimensional difference in gel electrophoresis (2D-DIGE) and mass spectrometry analyses identified a total of 17 different proteins that were significantly affected by supraphysiological levels of AAS. Furthermore, pathway analyses showed that modulated proteins were mainly associated to glucose metabolism, drug detoxification, stress response and cell cycle. Validation of many of these proteins, such as GSTM1, ERH, GAPDH, PEBP1 and PDIA6, were confirmed by western blotting. We further demonstrated that AAS exposure decreased expression of estrogen receptors and GnRH, while two important signaling pathway proteins p-ERK, and p-p38, were modulated. Our results suggest that steroids have the capacity to directly affect the neuroendocrine system by modulating key cellular processes for the control of reproductive function.

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“…However, candidate gene studies are often non-reproducible, because the effects of candidate markers are specific for some populations (Georges 2007). In addition, 43 candidate genes showed positive association between DNA sequence variation (base substitutions, indels, the number of trinucleotide repeats) and human male infertility phenotypes (Fritsche et al 1998;Holyoake et al 1999;Ramanujam et al 2000;van der Ven et al 2000;Kucera et al 2001;Rovio et al 2001;Pan et al 2002;Teng et al 2002;Tsujimura et al 2002;Maiorino et al 2003;Tanaka et al 2003;Jensen et al 2004;Watanabe et al 2004;Westerveld et al 2004;Ahda et al 2005;Galan et al 2005;Miyagawa et al 2005;Paduch et al 2005;Stouffs et al 2005;Vouk et al 2005;Aoki et al 2006;Christensen et al 2006;Galan et al 2006;Lee et al 2006;Peterlin et al 2006;Rani et al 2006;Sato et al 2006;Tsujimura et al 2006;Westerveld et al 2006;Zhoucun et al 2006a,b;Bonache et al 2007;Dhillon et al 2007;Lu et al 2007;Onaran et al 2007;Ravel et al 2007;Tu¨ttelmann et al 2007;Gunel-Ozcan et al 2008;…”

Section: Association Studiesmentioning

confidence: 99%

Comparative Genomics Approach to Identify Candidate Genetic Loci for Male Fertility

Ogorevc

Dovč

Kunej

2011

Reprod Domestic Animals

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The article presents multi-species, genome-wide, comparative approach to review male fertility-associated loci to contribute to the development of new genetic markers that could be of interest for functional studies and have the potential to be implemented in farm animal breeding programmes. We reviewed 835 male fertility-associated candidate loci from seven species and presented them as bovine orthologues where possible. The candidate loci were identified exploiting seven different research approaches: (i) data from animal models: mouse transgenics and knock-outs (569 genes) and random chemical mutagenesis of mouse genome (31); (ii) animal QTL (69); (iii) genes differentially expressed between fertile and subfertile phenotype in humans and mouse (95); (iv) DNA sequence variations that show specific allele-phenotype interactions (43 in human and 13 in farm animals); (v) germ line-specific small non-coding RNAs (47); (vi) testes expressed genes controlling complex differentiation process of mammalian spermatogenesis (6); and (vii) epigenetically regulated genes (4). According to the number of different research approaches reporting effects of individual genes, we selected 33 most promising candidate genes, which were further in silico analysed for expression levels in testes, genetic variability and top biological functions in functional networks. The aim of this study was to review systematically male fertility-associated candidate loci using integrated information from different study approaches and species, which will further facilitate development of novel genetic markers for selection towards improved fertility in domestic animals.

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Relationships Between Levels of Estradiol and Testosterone in Seminal Plasma andGSTM1Polymorphism in Infertile Men

Cited by 11 publications

References 28 publications

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

Differential protein expression profile in the hypothalamic GT1-7 cell line after exposure to anabolic androgenic steroids

Comparative Genomics Approach to Identify Candidate Genetic Loci for Male Fertility

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