Mass Regeneration of Shoots from Cut Surfaces of Stems in Tomato Stock Plants

Harada, Masashi; Oda, Masayuki; Mori, Genjiro; Ikeda, Hideo

doi:10.2503/jjshs.74.479

Cited by 14 publications

(11 citation statements)

References 4 publications

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“…The complete decapitation method (CDM), which regenerates multiple shoots from the cut surfaces of the main and lateral stems of plants grown in vivo, was reported as a new vegetative propagation method in tomato plants (Harada et al, 2005). The authors reported that 79 shoots regenerated from calluses on the stock plant 36 d after decapitation.…”

Section: Tomato Plants (Lycopersicon Esculentummentioning

confidence: 99%

In Vivo Shoot Regeneration Promoted by Shading the Cut Surface of the Stem in Tomato Plants

Johkan¹,

Mori²,

Mitsukuri³

et al. 2008

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The aim of this study was to establish an alternative method to produce clones of tomato plants by modification of the complete decapitation method, which regenerates multiple shoots from the cut surfaces of the main and lateral stems of plants grown in vivo. Shading the stems of tomato plants drastically increased the number of regenerated shoots from 2.4 in controls with unshaded stems to 36.2 in shaded stems. In shaded stems, the concentrations of chlorophyll and phenolic compounds were stable for 3 weeks after cutting, whereas these amounts increased in unshaded stems. Inhibiting the production of phenolic compounds in the shaded stem tissues was associated with an acceleration of shoot formation in vivo.

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Section: Tomato Plants (Lycopersicon Esculentummentioning

confidence: 99%

In Vivo Shoot Regeneration Promoted by Shading the Cut Surface of the Stem in Tomato Plants

Johkan¹,

Mori²,

Mitsukuri³

et al. 2008

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“…The aim of this study was to establish an efficient in vitro method of eggplant culture for adventitious shoot regeneration without PGRs and inhibition of rooting from the regenerated shoot. Recently, the complete decapitation method (CDM) was developed for mass propagation in tomato plants grown in an open field (Harada et al 2005), and the efficiency of the method was improved by Johkan et al (2008a, b, c). In this method the main and lateral stems are excised, which enables adventitious shoot regeneration from the cut ends of stems in vivo.…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro

et al. 2013

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Eggplant (Solanum melongena) seedlings cultured in vitro were excised at the center of the hypocotyl to generate decapitated seedlings with intact roots. This modification of the complete decapitation method (CDM) developed in vivo by Harada et al. (2005) allowed in vitro culture (CDM in vitro; CDMi). As controls, rootless hypocotyl segment explants (approximately 1 cm) and cotyledon explants were cultured on media supplemented with 4.4 µM 6-benzyladenin (BA) and 0.2 μM thidiazuron (TDZ), respectively. Cotyledon explants had formed calli 2 weeks after excision but did not develop adventitious buds, despite the use of optimal conditions reported previously for a different eggplant cultivar. Calli formed at the cut ends of hypocotyls 1 week after excision in both CDMi and hypocotyl cultures, and adventitious buds regenerated 1 week earlier in CDMi. Six weeks after excision, CDMi yielded 11.4 adventitious buds per explant, but only 4.1 formed in hypocotyl culture. Moreover, shoots longer than 1 cm developed 2 weeks earlier in CDMi than in hypocotyl culture. The number of shoots per explant was 8.1 in CDMi, but only 2.4 in hypocotyl culture 6 weeks after cutting. All shoots that developed were rooted on MS medium in CDMi, but only 71% of shoots formed roots in hypocotyl culture. These results indicate that intact roots are important for explant shoot regeneration and development, and CDMi is a simple and efficient method for obtaining multiple shoots without the need to determine optimal concentrations of plant growth regulators and overcome inhibition of rooting in the obtained shoots.

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“…This method regenerated 79 shoots from calli on the main and lateral stems of a single stock plant, with seven true leaves unfolding 36 d after cutting (Harada et al, 2005). The propagation efficiency of CDM is higher than that of harvesting lateral shoots, but lower than that of seed propagation.…”

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confidence: 96%