Maslinic acid, a triterpenic anti-tumoural agent, interferes with cytoskeleton protein expression in HT29 human colon-cancer cells

Rufino‐Palomares, Eva E.; Reyes‐Zurita, Fernando J.; Garcı́a-Salguero, Leticia; Mokhtari, Khalida; Medina, Pedro P.; Lupiáñez, José A.; Peragón, Juan

doi:10.1016/j.jprot.2013.02.031

Cited by 69 publications

(47 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, this fraction selectively induces apoptosis in HT-29 colon cancer cells by a JNK-p53-dependent mechanism (17), as well as induces significant changes in the cytoskeleton of this human colon cancer cell line (18). The effect on cell proliferation and apoptosis exerted by olive oil in HT-29 cells has been considered due to some components of the skin of olives, which contains pentacyclic triterpenes, that is, maslinic and oleanolic acids (19).…”

Section: Effects Of Oils and Faees On Ht-29 Cells Viabilitymentioning

confidence: 99%

Various Acylglycerols from Common Oils Exert Different Antitumor Activities on Colorectal Cancer Cells

Ramos‐Bueno

González‐Fernández

Guil‐Guerrero

2016

Nutrition and Cancer

View full text Add to dashboard Cite

Colorectal cancer is one of the leading causes of death in Western countries; therefore, the implementation of healthy dietary habits in order to prevent its occurrence is a desirable action. We show here that both free fatty acids (FFAs) and some acylglycerols induce antitumoral actions in the colorectal cancer cell line HT-29. We tested several C18 polyunsaturated fatty acid-enriched oils (e.g., sunflower and Echium) as well as other oils, such as arachidonic acid-enriched (Arasco®) and docosahexaenoic acid-enriched (Marinol® and cod liver oil), in addition to coconut and olive oils. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test indicated inhibitory effects on HT-29 cells viability for FFAs, and monoacylglycerol and diacylglycerol (DAG) species, while the lactate dehydrogenase test proved that FFAs were the more effective species to induce membrane injury. Conversely, all species did not exhibit actions on CCD-18 normal human colon cells viability. Furthermore, transmission electron microscopy showed the presence of necrosis and apoptosis, while the monoacylglycerol lipase (MAGL) inhibition test demonstrated high activity for 2-monoacylglycerols derived from Arasco and sunflower oils. However, different monoacylglycerols and DAGs have also the potential for MAGL inhibition. Therefore, checking for activity on colon cancer cells of specifically designed acylglycerol-derivative species would be a suitable way to design functional foods destined to avoid colorectal cancer initiation.

show abstract

Section: Effects Of Oils and Faees On Ht-29 Cells Viabilitymentioning

confidence: 99%

Various Acylglycerols from Common Oils Exert Different Antitumor Activities on Colorectal Cancer Cells

Ramos‐Bueno

González‐Fernández

Guil‐Guerrero

2016

Nutrition and Cancer

View full text Add to dashboard Cite

show abstract

“…Thus far, oleanolic acid and maslinic acid are known to have beneficial properties against different types of cancers [114,117,118,119,120,121,122,123,124]. Recently, Sánchez-Quesada et al [114] sought to elucidate whether both compounds possessed chemopreventive activity on two cell lines of human breast cancer cells (MDA-MB-231 and MCF7) (Table 4).…”

Section: Olive Oil and Phenolic Compoundsmentioning

confidence: 99%

Phenolic Compounds Isolated from Olive Oil as Nutraceutical Tools for the Prevention and Management of Cancer and Cardiovascular Diseases

Reboredo‐Rodríguez

Varela-López

Forbes-Hernández

et al. 2018

IJMS

View full text Add to dashboard Cite

Non-communicable diseases (NCDs) have become the largest contributor to worldwide morbidity and mortality. Among them, cancer and cardiovascular diseases (CVDs) are responsible for a 47% of worldwide mortality. In general, preventive approaches modifying lifestyle are more cost-effective than treatments after disease onset. In this sense, a healthy diet could help a range of NCDs, such as cancer and CVDs. Traditional Mediterranean Diet (MD) is associated by the low-prevalence of certain types of cancers and CVDs, where olive oil plays an important role. In fact, different epidemiological studies suggest that olive oil consumption prevents some cancers, as well as coronary heart diseases and stroke incidence and mortality. Historically, the beneficial health effects of virgin olive oil (VOO) intake were first attributed to the high concentration of monounsaturated fatty acids. Nowadays, many studies indicate that phenolic compounds contained in olive oil have positive effects on different biomarkers related to health. Among them, phenolic compounds would be partially responsible for health benefits. The present work aims to explore, in studies published during the last five years, the effects of the main phenolic compounds isolated from olive oil on different cancer or CVD aspects, in order to clarify which compounds have more potential to be used as nutraceuticals with preventive or even therapeutic properties.

show abstract

“…Maslinic acid has been revealed to exert therapeutic effects on a variety of solid tumors, including in bladder, prostate, colon, esophageal, colorectal, cervical and ovarian cancer (5)(6)(7)(8). However, the molecular mechanisms underlying the antitumor functions of maslinic acid remain elusive.…”

Section: Introductionmentioning

confidence: 99%

Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway

Wang¹,

Tang²,

Zhang³

2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. The present study aimed to determine whether maslinic acid effectively inhibits the proliferation of MKN28 cells, and to investigate the mechanisms underlying its antitumor functions. MKN28 cell viability was evaluated using a Cell Counting Kit-8, cell proliferation was analyzed by a colony formation assay and flow cytometry was used to investigate the rate of apoptosis. Western blot analysis was performed in order to determine the differential expression levels of Janus kinase (JAK), signal transducer and activator of transcription 3 (STAT3) and apoptosis associated proteins B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax) and Bcl-2 associated agonist of cell death (Bad). Interleukin-6 (IL-6) concentration was evaluated using ELISA. IL-6 and anti-IL-6 antibodies were used to investigate the role of IL-6 in MKN28 cells treated with maslinic acid proliferation, and the STAT3 phosphorylation rates. The results demonstrated that maslinic acid treatment significantly reduced cell proliferation, induced apoptosis and was accompanied by a significant decrease in Bcl-2, Bax and Bad expression levels. Maslinic acid treatment also resulted in the downregulation of phosphorylated-STAT3 and JAK2, and significantly inhibited the protein expression of IL-6. Maslinic acid is able to inhibit MKN28 cell proliferation and the phosphorylation of STAT3 by downregulating the expression of IL-6. These results suggest that maslinic acid suppresses the growth of MKN28 cells by inducing apoptosis via its inhibition of the IL-6/JAK/STAT3 signaling cascade.

show abstract

Maslinic acid, a triterpenic anti-tumoural agent, interferes with cytoskeleton protein expression in HT29 human colon-cancer cells

Cited by 69 publications

References 38 publications

Various Acylglycerols from Common Oils Exert Different Antitumor Activities on Colorectal Cancer Cells

Various Acylglycerols from Common Oils Exert Different Antitumor Activities on Colorectal Cancer Cells

Phenolic Compounds Isolated from Olive Oil as Nutraceutical Tools for the Prevention and Management of Cancer and Cardiovascular Diseases

Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway

Contact Info

Product

Resources

About