Masking of co‐operativity of nucleotide sites in pig heart mitochondrial ATPase (F₁) by heating

"Hysteretic" behavior and nucleotide binding sites of pig heart mitochondrial F1 adenosine 5'-triphosphatase

“…The existence of regulatory site(s) has indeed been proposed previously on the basis of kinetic experiments (Godinot et al, 1975;Schuster et al, 1975;Leimgruber & Senior, 1976;Recktenwald & Hess, 1977;. The binding of ADP to the control site could very well account at least partly for the regulations observed in kinetic experiments.…”

“…of 1 mol of ADP/mol of enzyme but does not modify the inhibition. With nucleotide-depleted F,, the binding of the liesults from this laboratory (Godinot et al, 1975;) and from others (Hilborn & Hammes, 1973; Schuster et al, 1975; Garrett & Penefsky, 1975b;Pedersen, 1975;Leimgruber & Senior, 1976;Recktenwald & Hess, 1977;Harris et al, 1978; Slater et al, 1979) have provided support for the view that regulatory nucleotide-binding sites, distinct from catalytic sites, are present in mitochondrial F[ adenosine 5'-triphosphatase (FJ.1 Numerous experimental data suggest that the catalytic sites are located on the ß subunits (Budker et al, 1977;Verschoor et al, 1977;Wagenvoord et al, 1977;Esch & Allison, 1978;Pougeois et al, 1979;Slater et al, 1979). The recent use of nucleotide analogues bearing azido groups (Russell et al, 1976) has shed light on nucleotide-site location.…”

Use of trypsin to monitor conformational changes of mitochondrial adenosine triphosphatase induced by nucleotides and phosphate

“…In other conditions, nucleotide-dependent conformational changes of FrATPase have been revealed by variations in aurovertin fluorescence (Chang & Penefsky, 1973), by modifications in thiol accessibility (Godinot et al, 1975(Godinot et al, , 1977,…”

Interaction between catalytic and regulatory sites of mitochondrial F1-ATPase as monitored by the differential effects of inhibitors and nucleotide analogs on the "hysteretic" behavior of the enzyme

“…The presence of regulatory sites has been recently shown by kinetic studies (Godinot et al, 1975;Schuster et al, 1975;Recktenwald & Hess, 1977;Roveri et al, 1980;Fleury et al, 1980) or by measuring the direct binding of ADP (Hilborn & Hammes, 1973;Pedersen, 1975) or nucleotide analogues (Garrett & Penefsky, 1975a,b; Di Pietro et al, 1979;Wagenvoord et al, 1979). Moyle & Mitchell (1975) have suggested as an hypothesis that the binding of ADP to a regulatory site might be involved in transitions from a catalytically active state to an inactive one.…”