Mary L. Ryan, AHIP, FMLA Medical Library Association 2008–2009

Doyle, Jacqueline Donaldson; Sollenberger, Julia; Plaisted, Dena K.

doi:10.3163/1536-5050.96.3.004

Cited by 1 publication

(1 citation statement)

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“…PEDV and TGEV have been associated with significant economic losses in farms [4,5]. PEDV was first observed among pigs in England in 1971 [6], whereas TGEV was isolated for the first time in 1946 [7]. Currently, PEDV and TGEV occur in parts of Europe, North America, and Asia [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens

et al. 2016

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In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.2 and 3.5 % of the samples, respectively, tested positive. Under optimized conditions, the duplex nanoPCR assay had a detection limit of 7.6 × 10 and 8.5 × 10 copies μL for PEDV and TGEV, respectively. The sensitivity of the duplex nanoPCR assay was ten times higher than that of a conventional PCR assay. Moreover, no fragments were amplified when the duplex nanoPCR assay was used to test samples containing other porcine viruses. Our results indicate that the duplex nanoPCR assay described here is useful for the rapid detection of PEDV and TGEV and can be applied in clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%