A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens

Zhu, Yu; Liang, Lin; Luo, Yakun; Wang, Guihua; Wang, Chun-Ren; Cui, Yudong; Xia, Ai; Cui, Shangjin

doi:10.1007/s11262-016-1405-z

Cited by 17 publications

(21 citation statements)

References 22 publications

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“…TGEV infection in pigs was reported globally between the late 1980s and the 1990s (Zuniga et al, 2016;Kemeny and Woods, 1977;Pritchard, 1987;Wood et al, 1981;Woods and Wesley, 1986). In recent years, mixed infections have occurred frequently with TGEV and PEDV Zhang et al, 2013;Zhu et al, 2017). In the present study, TGEV JS2012 strain was obtained from a farm with a diarrhea outbreak, in which TGEV and PEDV co-infection was detected by RT-PCR.…”

Section: Discussionmentioning

confidence: 91%

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Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China

et al. 2020

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A B S T R A C TPorcine transmissible gastroenteritis virus (TGEV) is one of the major etiological agents of viral enteritis and fetal diarrhea in suckling piglets. In this study, a TGEV JS2012 strain was isolated from the feces of piglets in Jiangsu Province, China. The phylogenetic analysis showed that TGEV JS2012 was placed between the Purdue and the Miller clusters. Analysis of recombination confirmed that TGEV JS2012 is a natural recombinant strain between Miller M6 and Purdue 115. Similar to Miller M6, virulent Purdue and China strain TS, in S gene the JS2012 maintained genetic integrity and the characteristics of the TGEV virulent strains. In vivo, TGEV JS2012 caused 100% mortality in newborn piglets, indicating the strong pathogenicity of this isolate. These results reveal that the JS2012 is a novel natural recombinant TGEV with high virulence. Our findings provide valuable information about genetic diversity and infection mechanism of the coronavirus family.

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Section: Discussionmentioning

confidence: 91%

“…There have been many recent reports of TGEV infection in China (Hou et al, 2012;Li et al, 2010;Zhang et al, 2017). Epidemiological investigations have shown that TGEV is often present in mixed infection with porcine epidemic diarrhea virus (PEDV) Zhang et al, 2013;Zhu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China

et al. 2020

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“…The main reasons responsible for this difference might be the varied geographic distributions of this virus or different sample types. Using nanoparticle‐assisted polymerase chain reaction, Zhu et al found a 3.5% positive rate of TGEV infection in 114 samples from four provinces of China (Zhu et al, ). Investigations from Zhao et al revealed that the TGEV infection rate was as low as 1.91% in East, Northeast and Southeast China between 2012 and 2014 (Zhao et al, ).…”

Section: Discussionmentioning

confidence: 99%

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Ding

et al. 2019

Transbound Emerg Dis

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The major enteric RNA viruses in pigs include porcine epidemic diarrhoea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (PRV‐A), porcine kobuvirus (PKV), porcine sapovirus (PSaV) and porcine deltacoronavirus (PDCoV). For differential diagnosis, a multiplex RT‐PCR method was established on the basis of the N genes of TGEV, PEDV and PDCoV, the VP7 gene of PRV‐A, and the polyprotein genes of PKV and PSaV. This multiplex RT‐PCR could specifically detect TGEV, PEDV, PDCoV, PRV‐A, PKV and PSaV without cross‐reaction to any other major viruses circulating in Chinese pig farms. The limit of detection of this method was as low as 100–101 ng cDNA of each virus. A total of 398 swine faecal samples collected from nine provinces of China between October 2015 and April 2017 were analysed by this established multiplex RT‐PCR. The results demonstrated that PDCoV (144/398), PSaV (114/398), PEDV (78/398) and PRV‐A (70/398) were the main pathogens, but TGEV was not found in the pig herds in China. In addition, dual infections, for example, PDCoV + PSaV, PDCoV + PRV‐A, PRA‐V + PSaV and PEDV + PDCoV, and triple infections, for example, PDCoV + PRV‐A + PSaV and PEDV + PDCoV + PKV, were found among the collected samples. The multiplex RT‐PCR provided a valuable tool for the differential diagnosis of swine enteric viruses circulating in Chinese pig farms and will facilitate the prevention and control of swine diarrhoea in China.

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“…3 To date, more than 170 genotypes of HPV have been identified and are categorized as high-, medium-, and low-risk according to their biological characters and oncogenic potential. Notably, constant highrisk HPV (HR-HPV) infection, in particular, HPV-16 and HPV- 18 subtypes, has been demonstrated as key players in inducing cervical carcinoma, which is the second common cancer in women among the world. 4 Theoretically, after infection, the HPV maintains its proliferation and protein synthesis depending on the replication of host cells after integrating its DNA into the host genome.…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive and specific nanoparticle‐assisted PCR assay for detecting HPV‐16 and HPV‐18 DNA

Liu

et al. 2020

Journal of Medical Virology

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Carcinoma precursor lesion caused by persistent infection of human papillomavirus (HPV) types 16 and 18 is known as a principal inducer of cervical cancer. Therefore, rapid and effective detection of HPV‐16 and HPV‐18 infection at early stage is an important strategy for preventing such disease. In this study, a novel duplex nanoparticle‐assisted polymerase chain reaction (nanoPCR) assay was developed to detect both of the two genotypes simultaneously. Two pairs of primers for nanoPCR were designed based on the conserved region within the early 6 (E6) gene of HPV‐16 and HPV‐18, respectively. After optimizing reaction conditions, the nanoPCR assay displayed 10‐fold more sensitive than that of conventional PCR and showed high specificity. The detection limit of nanoPCR was 1.7 × 101 copies/μL for HPV‐16, 1.2 × 102 copies/μL for HPV‐18, and no cross‐reaction was detected after using other viruses or HPV subtypes as templates. Of 209 clinical samples collected from patients, as also confirmed by sequencing, the nanoPCR method gave consistent results with conventional PCR assay: 7 positives for HPV‐16, 4 positives for HPV‐18, and no co‐infection. Here is the first report to introduce a reproducible nanoPCR assay for detecting HPV DNA with high sensitivity and specificity, which may point out a useful diagnostic tool for potential clinical application.

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A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens

Cited by 17 publications

References 22 publications

Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China

Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Development of a sensitive and specific nanoparticle‐assisted PCR assay for detecting HPV‐16 and HPV‐18 DNA

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