Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite

Peter, Susan J.; Lu, Lichun; Kim, Daniel; Mikos, Antonios G.

doi:10.1016/s0142-9612(99)00254-9

Cited by 160 publications

(113 citation statements)

References 15 publications

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“…The compressive moduli range from 135±18 to 150±40 MPa without significant differences among samples. Unlike previously reported results of increased mechanical strength of crosslinked PPF composites by adding calcium phosphate such as β-TCP [33,34], in this study the addition of HA nanoparticles does not increase mechanical strength of the final crosslinked PPF/HA nanocomposites. It can be interpreted by the fact that PPF used in this study has a fairly high molecular weight to render a rigid crosslinked PPF with a compressive modulus of over 100 MPa.…”

Section: Structural Characterizations and Morphologycontrasting

confidence: 96%

“…By controlling the molecular weight of PPF and the crosslinking density, the maximum compressive strength and modulus of crosslinked PPF can be over 10 and 100 MPa, respectively [29][30][31][32]. Although many previous studies have been performed to enhance both mechanical strength and osteoconductive properties by adding calcium phosphate such as β-tricalcium phosphate (β-TCP) to PPF [33,34], or preparing PPF/HA composites by either simple mixing [35] or from in situ precipitation [36], the correlation between the physical properties and cell responses on PPF/HA nanocomposites has not yet been reported, compared with other poly(α-hydroxyl acids)-based composites.…”

Section: Introductionmentioning

confidence: 99%

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Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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A series of crosslinkable nanocomposites has been developed using hydroxyapatite (HA) nanoparticles and poly(propylene fumarate) (PPF). PPF/HA nanocomposites with four different weight fractions of HA nanoparticles have been characterized in terms of thermal and mechanical properties. To assess surface chemistry of crosslinked PPF/HA nanocomposites, their hydrophilicity and capability of adsorbing proteins have been determined using static contact angle measurement and MicroBCA protein assay kit after incubation with 10% fetal bovine serum (FBS), respectively. In vitro cell studies have been performed using MC3T3-E1 mouse pre-osteoblast cells to investigate the ability of PPF/HA nanocomposites to support cell attachment, spreading, and proliferation after 1, 4, and 7 days. By adding HA nanoparticles to PPF, the mechanical properties of crosslinked PPF/ HA nanocomposites have not been increased due to the initially high modulus of crosslinked PPF. However, hydrophilicity and serum protein adsorption on the surface of nanocomposites have been significantly increased, resulting in enhanced cell attachment, spreading, and proliferation after 4 days of cell seeding. These results indicate that crosslinkable PPF/HA nanocomposites are useful for hard tissue replacement because of excellent mechanical strength and osteoconductivity.

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Section: Structural Characterizations and Morphologycontrasting

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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“…Recently developed porous composite scaffolds have been formed in situ by gas foaming, with up to 61% porosity, 50-500 m pores, and a compressive modulus of . PPF biomaterials have been shown to support osteoblast attachment and proliferation in vitro, and ingrowth of new bone tissue in vivo (16)(17)(18)(19). Growth factors have been incorporated via PLGA micro-spheres into poly(propylene fumarate) materials for controlled release (20).…”

Section: Introductionmentioning

confidence: 99%

Injectable Biodegradable Polyurethane Scaffolds with Release of Platelet-derived Growth Factor for Tissue Repair and Regeneration

et al. 2008

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Purpose-The purpose of this work was to investigate the effects of triisocyanate composition on the biological and mechanical properties of biodegradable, injectable polyurethane scaffolds for bone and soft tissue engineering.Methods-Scaffolds were synthesized using reactive liquid molding techniques, and were characterized in vivo in a rat subcutaneous model. Porosity, dynamic mechanical properties, degradation rate, and release of growth factors were also measured.Results-Polyurethane scaffolds were elastomers with tunable damping properties and degradation rates, and they supported cellular infiltration and generation of new tissue. The scaffolds showed a two-stage release profile of platelet-derived growth factor, characterized by a 75% burst release within the first 24 h and slower release thereafter.Conclusions-Biodegradable polyurethanes synthesized from triisocyanates exhibited tunable and superior mechanical properties compared to materials synthesized from lysine diisocyanates. Due to their injectability, biocompatibility, tunable degradation, and potential for release of growth factors, these materials are potentially promising therapies for tissue engineering.

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“…Bone marrow stromal cell isolation and preculture Primary cells were freshly isolated from 8 Fischer 344 male rats (6-8 weeks old, 150-165 g), as previously described (Peter et al, 2000). Briefl y, femora and tibiae were excised from the rats under aseptic conditions, and the bone marrow was fl ushed from the bone with primary media.…”

Section: Characterization Of Cap Nanoparticlesmentioning

confidence: 99%

Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles: in vitro evaluation of cell behavior

Bongio

Beucken

Nejadnik

et al. 2011

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The ultimate goal of this work was to develop a biocompatible and biomimetic in situ crosslinkable hydrogel scaffold with an instructive capacity for bone regenerative treatment. To this end, synthetic hydrogels were functionalized with two key components of the extracellular matrix of native bone tissue, i.e. the three-amino acid peptide sequence RGD (which is the principal integrin-binding domain responsible for cell adhesion and survival of anchorage-dependent cells) and calcium phosphate (CaP) nanoparticles in the form of hydroxyapatite (which are similar to the inorganic phase of bone tissue). Rat bone marrow osteoblast-like cells (OBLCs) were encapsulated in four different biomaterials (plain oligo(poly(ethylene glycol) fumarate) (OPF), RGDmodifi ed OPF, OPF enriched with CaP nanoparticles and RGD-modifi ed OPF enriched with CaP nanoparticles) and cell survival, cell spreading, proliferation and mineralized matrix formation were determined via cell viability assay, histology and biochemical analysis for alkaline phosphatase activity and calcium. This study showed that RGD peptide sequences promoted cell spreading in OPF hydrogels and hence play a crucial role in cell survival during the early stage of culture, whereas CaP nanoparticles signifi cantly enhanced cell-mediated hydrogel mineralization. Although cell spreading and proliferation activity were inhibited, the combined effect of RGD peptide sequences and CaP nanoparticles within OPF hydrogel systems elicited a better biological response than that of the individual components. Specifi cally, both a sustained cell viability and mineralized matrix production mediated by encapsulated OBLCs were observed within these novel biomimetic composite systems.

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Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite

Cited by 160 publications

References 15 publications

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Injectable Biodegradable Polyurethane Scaffolds with Release of Platelet-derived Growth Factor for Tissue Repair and Regeneration

Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles: in vitro evaluation of cell behavior

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