Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy

Paprocki, J; Sutkowy, Paweł; Piechocki, Jacek; Woźniak, Alina

doi:10.1155/2019/8472346

Cited by 12 publications

(13 citation statements)

References 31 publications

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“…Also, there were no changes in the GPx activity in erythrocytes following a procedure in the hyperbaric chamber, but the activity of this enzyme prior to treatment in patients who had previously used hyperbaric chamber many times was significantly lower than in patients who had received HBO treatment no more than three times [30]. In patients with sudden sensorineural hearing loss, a decrease in the CAT activity in erythrocytes was observed after the first procedure in the hyperbaric chamber, the SOD activity in erythrocytes decreased after the 14th procedure, and the GPx activity increased after the 14th procedure [34].…”

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confidence: 87%

Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

Paprocki

Pawłowska

Sutkowy

et al. 2020

Oxidative Medicine and Cellular Longevity

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Objective. To determine the concentration of thiobarbituric acid reactive substances (TBARS) in erythrocytes and blood plasma, and the activities of selected antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in erythrocytes in patients receiving hyperbaric oxygen (HBO) treatment due to difficult-to-heal “skin wounds”. Material and Methods. Indices of oxidative stress were assessed in venous blood taken from 23 patients three times: immediately before HBO procedure, approx. 5 minutes after leaving the hyperbaric chamber, and after 25 HBO procedures. Moreover, selected blood counts were measured in the collected material two times: prior to treatment and after 25 HBO procedures. Results. A statistically significant positive correlation between the CAT activity and the TBARS concentration in the erythrocytes of patients was found before treatment in the hyperbaric chamber (r=0.394; P≤0.05). No statistically significant changes in the TBARS concentration in erythrocytes and blood plasma were observed both after the first HBO procedure and after 25 procedures. No statistically significant changes in the activities of CAT, SOD, and GPx were noted. Platelet count decreased by 18.7% (P≤0.05) after 25 HBO procedures. Granulocyte count decreased by approx. 21% (P≤0.05), and granulocyte percentage by 11.8% (P≤0.01). In turn, the percentage of lymphocytes and monocytes increased after the treatment by 16.6% (P<0.05) and 16.4% (P<0.05), respectively. Conclusions. Exposure to HBO due to difficult-to-heal skin wounds does not significantly affect the levels of oxidative stress in the peripheral blood of patients and, from the point of view of oxidation–reduction processes, appears to be a safe therapeutic method for the treatment of chronic wounds.

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confidence: 87%

Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

Paprocki

Pawłowska

Sutkowy

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…However, the mechanism remains unclear. It is likely that mitophagy is related to the ability of HBO to enhance antioxidant enzyme activity and reduce edema in the nervous tissue [28]. Moreover, HBO promotes capillary regeneration and improves microcirculation.…”

Section: Discussionmentioning

confidence: 99%

DNA Methylation May be Involved in the Analgesic Effect of Hyperbaric Oxygen via Regulating FUNDC1

Liu

Gao

et al. 2020

Pain Research and Management

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Background. Neuropathic pain (NP) is a type of chronic pain which lacks predictable, effective, and safe therapeutic options. We investigated the role of hyperbaric oxygen (HBO) in expression of FUN14 domain-containing 1 (FUNDC1), which is associated with DNA methylation. Methods. We randomly divided rats into four groups: sham operation (S), S + HBO, chronic constriction injury (CCI), and CCI + HBO. Lumbar (L)4 and L5 dorsal root ganglia (DRGs) were used to assess expression of DNA methyltransferase (DNMT)1, DNMT3a, and DNMT3b by western blotting and RT-PCR. Pain-related behaviors were evaluated using mechanical withdrawal threshold and thermal withdrawal latency analysis. Western blotting was also used to assess expression of FUNDC1, BCL2, and adenovirus E1B19 kDa-interacting protein 3-like (NIX) and BCL2 and adenovirus E1B19 kDa-interacting protein3 (BNIP3). And we also examined the changes of FUNDC1 with immunofluorescence. Nonnucleoside DNA methyltransferase inhibitor RG108 was administered prior to CCI. The pain-related behavior and western blotting changes were examined in all groups. Results. DNMT3a expression was higher on day 14 after CCI. HBO downregulated DNMT3a mRNA and protein expression, but not those of DNMT1 and DNMT3b. HBO increased pain-related behavior significantly, while it was down-regulated by RG108. In HBO groups, FUNDC1, NIX, and BNIP3 expression was upregulated more significantly than in the CCI group. In addition, FUNDC1 protein colocalized with NeuN and rarely with glutamine synthetase. However, expression was reduced when RG108 was administered. Immunofluorescence showed that FUNDC1 was upregulated after HBO treatment. Conclusion. Our findings suggest that DNA methylation is involved in the analgesic effect of HBO via the regulation of FUNDC1.

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“…The use of HBOT has increased markedly in various diseases over the past decade [1]. The etiology for sudden sensorineural hearing loss (SSNHL) is defined in only 10 % of cases, whereas the rest are labeled as idiopathic [2][3][4]. So, how can be HBOT effective with a common molecular pathway in treatment of SSNHL?…”

Section: Introductionmentioning

confidence: 99%

“…The reason for the etiology of sudden hearing loss is not found in many patients and is considered idiopathic [5]. Little is known about the association between SSNHL and oxidative stress [3]. In addition, endothelial dysfunction and vascular diseases are also involved in etiopathogenesis of SSNHL [6].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the feeding of the cochlea from the posterior cerebral circulation with a single terminal branch may explain the sudden and rapid course in the SSNHL of vascular etiopathogenesis. In addition, many clinical studies have shown that SSNHL patients often present with systemic arterial hypertension, diabetes mellitus and dyslipidemias with atherosclerosis, and thromboembolic risk [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Compensatory Paraoxonase and Arylesterase Levels in Hyperbaric Oxygen Treatment of Sudden Sensorineural Hearing Loss

Eren

Yılmaz

Yıldırım³

et al. 2020

Polish Hyperbaric Research

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Objective: Paraoxonase1 (PON1) and Arylesterase (ARE) levels are associated with reduced risk of atherosclerosis. The functional status of high density lipoprotein (HDL) is closely related to the PON1/ARE enzyme activity. Functional changes in treatment of sudden sensorineural hearing loss (SSNHL) may be achieved by post-translational modification of lipid metabolism induced by hyperbaric oxygen therapy (HBOT).Methods: Men patients with SSNHL who met the research criteria were included in the study. HBOT was performed on average 30 sessions. Laboratory measurements were made at the beginning and end of HBOT for the same patients. Serum levels of PON1/ARE and routine lipid laboratory parameters were measured to determine possible changes in SSNHL after HBOT.Results: In this study, a reducing effect on PON1 enzyme amount of long-term HBOT was detected. The serum PON1 amount of patients with SSNHL was 19.7 ± 2.7 ng / mL (mean ± SD) before HBOT, and the serum PON1 decreased to 17.0 ± 2.1 ng / mL (mean ± SD) after 30 sessions of HBOT. This decrease in PON1 levels was statistically significant (p =0.035). There was also a statistically significant decrease in the enzyme activity of ARE in the SSNHL patients (p=0.024).Conclusion: This preliminary study showed a significant decrease in serum PON1/ARE enzyme content in SSNLH patients with HBOT. In fact, it can be assumed that HBOT has no adverse effect on HDL functionality. However, the decrease in PON1 level by HBOT with 30 or more sessions may be important for the antioxidant function of HDL.It may possibly cause post-translational changes in antioxidant defense mechanisms due to increased oxidative stress with HBOT. In conclusion, larger clinical studies are needed to determine the possible effects of HBOT on HDL-related PON1/ARE functionality in SSNHL.

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Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy

Cited by 12 publications

References 31 publications

Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

DNA Methylation May be Involved in the Analgesic Effect of Hyperbaric Oxygen via Regulating FUNDC1

Compensatory Paraoxonase and Arylesterase Levels in Hyperbaric Oxygen Treatment of Sudden Sensorineural Hearing Loss

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