Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

Abstract

Objective. To determine the concentration of thiobarbituric acid reactive substances (TBARS) in erythrocytes and blood plasma, and the activities of selected antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in erythrocytes in patients receiving hyperbaric oxygen (HBO) treatment due to difficult-to-heal “skin wounds”. Material and Methods. Indices of oxidative stress were assessed in venous blood taken from 23 patients three times: immediately before HBO procedure, approx. 5 minutes after leaving the hyperbaric chamber, and after 25 HBO procedures. Moreover, selected blood counts were measured in the collected material two times: prior to treatment and after 25 HBO procedures. Results. A statistically significant positive correlation between the CAT activity and the TBARS concentration in the erythrocytes of patients was found before treatment in the hyperbaric chamber (r=0.394; P≤0.05). No statistically significant changes in the TBARS concentration in erythrocytes and blood plasma were observed both after the first HBO procedure and after 25 procedures. No statistically significant changes in the activities of CAT, SOD, and GPx were noted. Platelet count decreased by 18.7% (P≤0.05) after 25 HBO procedures. Granulocyte count decreased by approx. 21% (P≤0.05), and granulocyte percentage by 11.8% (P≤0.01). In turn, the percentage of lymphocytes and monocytes increased after the treatment by 16.6% (P<0.05) and 16.4% (P<0.05), respectively. Conclusions. Exposure to HBO due to difficult-to-heal skin wounds does not significantly affect the levels of oxidative stress in the peripheral blood of patients and, from the point of view of oxidation–reduction processes, appears to be a safe therapeutic method for the treatment of chronic wounds.

Hyperbaric Oxygen Ameliorates The Expression of Tumor Growth Factor-β and Malondialdehyde in Pristane-induced Lupus Nephritis Mice Model

“…The outcome showed a decrease which was significant in MDA between the last session of day 20 and day one. (27) Although using a lower HBO pressure and shorter time per session, as well as a longer duration of therapy, the results are the same as this study which showed that HBO therapy could reduce MDA levels.…”

“…(26) This treatment increments the expression of diverse growth factors. (27) The use of HBO therapy shows a hormetic effect by inducing reactive oxygen species (ROS) at moderate levels. (28) Moderate concentrations of ROS are useful for cell signaling and the immune system.…”

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing

“…Diabetic ulceration is a common diabetic complication, and it causes chronic wound infection that is a serious medical problem that threatens the health and quality of life of diabetic patients [ 3 – 5 ]. ROS are generated during incomplete oxygen metabolism, and under diabetic conditions, immune cells increase ROS levels in the wound microenvironment, leading to stubborn scars and prolonged wounds [ 6 – 8 ]. The superfluous ROS within the impaired wound can promote intense inflammatory reactions to make the wounds fragile, but also restricts skin regeneration by stem cells and macrophages [ 9 ].…”

“…To overcome these challenges, it is crucial to develop effective treatments that can simultaneously target bacterial infections and scavenge excessive ROS, promoting the recovery of diabetic wounds.In cellular enzyme-involved metabolism, oxygen undergoes a series of one-electron reactions, which alternately leads to the formation of several kinds of ROS, including superoxide anion (O 2 •− ), hydroxyl radical (OH·), and hydrogen peroxide (H 2 O 2 ). To defend against excessive ROS, the body has endogenous antioxidases, such as catalase (CAT), SOD, and GPx, are capable of catalyzing the disproportionation reactions of O 2 •− and H 2 O 2 into H 2 O [ 6 , 13 , 14 ]. However, these natural enzymes are usually unstable, with a short half-life in circulation, and are hard to be adequately produced in wound [ 15 ].…”