Marker-assisted screening of promising forms in the strawberry breeding

Лыжин, А. С.; Luk’yanchuk, I. V.

doi:10.1051/e3sconf/202125403002

E3S Web Conf.

2021

DOI: 10.1051/e3sconf/202125403002

|View full text |Cite

Marker-assisted screening of promising forms in the strawberry breeding

А. С. Лыжин¹,

I. V. Luk’yanchuk²

Abstract: The results of the use diagnostic DNA-markers in the breeding of strawberry (Fragaria × ananassa Duch.) were shown. The carriers of target alleles of red stele root rot resistance (F. virginiana Duch. ssp. platypetala, Bylinnaya, 69-29 (Feyerverk × Bylinnaya), 72-24 and 72-71 (Privlekatelnaya × Bylinnaya)), anthracnose resistance (Borovitskaya, Sudarushka, Elianny, Troubadour, 933-4 (F. virginiana Duch. ssp. platypetala × Rubinovyy kulon)), high mesifurane content in fruits (F. orientalis Los., F. moschata Duc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 16 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Analysis of the inheritance of the marker SCAR-R1A, linked to the Rpf1 red stele root rot resistance gene, in strawberry hybrid progeny

Лыжин

Luk’yanchuk

2022

Trudy po prikladnoy botanike, genetike i selektsii

View full text Add to dashboard Cite

Background. Resistance to pathogens is an important breeding trait of a cultivar. Red stele root rot (Phytophthora fragariae var. fragariae Hickman) is a dangerous root disease. Revealing the patterns of resistance inheritance and identifying promising genotypes is an important stage in the development of strawberry cultivars resistant to red stele root rot. The purpose of the study was to identify patterns of inheritance for the SCAR-R1A marker, linked to the Rpf1 red stele root rot resistance gene, in the strawberry hybrid combinations.Materials and methods. The target materials were the strawberry cultivars ‘Bylinnaya’, ‘Olimpiyskaya Nadezhda’, ‘Privlekatelnaya’ and ‘Feyyerverk’, and hybrid seedlings of the cross combinations Bylinnaya × Olimpiyskaya Nadezhda, Bylinnaya × Feyyerverk, Olimpiyskaya Nadezhda × Bylinnaya, Privlekatelnaya × Bylinnaya, and Feyyerverk × Bylinnaya. The Rpf1 gene was identified with the marker SCAR-R1A.Results and conclusion. For the hybrid combination Bylinnaya × Olimpiyskaya Nadezhda, the percentage of seedlings with an Rpf1 resistance allele was 33.3%. For the combination Bylinnaya × Feyyerverk, their percentage was 37.2%; for Olimpiyskaya Nadezhda × Bylinnaya, 39.4%; for Privlekatelnaya × Bylinnaya, 39.6%; and for Feyyerverk × Bylinnaya, 36.2%. The average percentage of seedlings with an Rpf1 allele for the studied combinations was 37.1%. Assessment of the compliance between the observed segregation and theoretical one according to the χ2 criterion confirmed the monogenic character of the studied trait and the Mendelian ratio of inheritance frequencies for the marker fragments of the Rpf1 gene as 1 : 1. Therefore, all identified seedlings with an Rpf1 allele are characterized by a heterozygous genotype. Strawberry hybrids promising for breeding were identified: 62-41 (Bylinnaya × Feyyerverk), 65-17, 65-24 (Olimpiyskaya Nadezhda × Bylinnaya), and 69-29 (Feyyerverk × Bylinnaya).

show abstract

Analysis of the inheritance of the marker SCAR-R1A, linked to the Rpf1 red stele root rot resistance gene, in strawberry hybrid progeny

Лыжин

Luk’yanchuk

2022

Trudy po prikladnoy botanike, genetike i selektsii

View full text Add to dashboard Cite

show abstract

Identification of <i>Rca2</i> and <i>Rpf1</i> resistance alleles in strawberry breeding forms of intervarietal hybrid origin

Lyzhin,

Luk’yanchuk

2024

Biotehnologiâ i selekciâ rastenij

View full text Add to dashboard Cite

Background. The creation of varieties with genetic resistance to fungal pathogens is one of the most important directions in strawberry breeding. A promising direction of accelerated selection of valuable specimens is molecular screening for the presence in their genotype of diagnostic DNA markers, linked to target genes of agrobiological traits. Objectives of the work were determination of the allelic state of the Rca2 (resistance to anthracnose) and Rpf1 (resistance to red stele root rot) genes in selected strawberry forms to identify genotypes resistant to Colletotrichum acutatum Simmonds and Phytophthora fragariae var. fragariae Hickman.Material and methods. The object of research was breeding forms of strawberries of intervarietal hybrid origin. As a result of 22 crossing combinations, 61 selection forms were obtained, in which the allelic composition of resistance genes was studied. The Rca2 anthracnose resistance allele was identified by means of the STS-Rca2_240 marker. The Rpf1 red stele root rot resistance allele was identified with the help of SCAR-R1A marker. Results. At least one of the two genes was identified in 20 selected strawberry forms (32.8%). The STS-Rca2_240 marker was identified in 15 strawberry seedlings (22.9%) from six hybrid combinations ‘Vicoda’ × ‘Roxana’, ‘Florence’ × ‘Faith’, ‘Asia’ × ‘Aprica’, ‘San Andreas’ × ‘Monterey’, ‘Alisa’ × ‘Quicky’, ‘Quicky’ × ‘Olympia’). The SCAR-R1A marker was identified in five strawberry seedlings (8.2%) from four hybrid combinations (‘Privlekatelnaya’ × ‘Bylinnaya’, ‘Feyerverk’ × ‘Bylinnaya’, ‘Bylinnaya’ × ‘Feyerverk’, ‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’). It has been established that all strawberry selected forms with identified marker fragments for resistance genes are characterized by a heterozygous combination of alleles.Conclusions. Promising sources of resistance alleles are strawberry selected forms: 2/1-24, 2/1-34 (‘Quicky’ × ‘Olympia’), 3/9-5 (‘Florence’ × ‘Faith’), 3/12-2, 3/12- 9 (‘Alisa’ × ‘Quicky’), 4/7-10, 4/7-19, 4/7-20 (‘Asia’ × ‘Aprica’), 5/2-26, 5/2-32 (‘San Andreas’ × ‘Monterey’), 9/ 1-11, 9/1-12, 9/1-13, 9/1-32, 9/1-37 ‘Vicoda’ × ‘Roxana’) in case of Rca2 gene; 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 69-29 (‘Feyerverk’ × ‘Bylinnaya’), 62-41 (‘Bylinnaya’ × ‘Feyerverk’), 61-15 (‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’) in case of Rpf1 gene.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

334 Leonard St

Brooklyn, NY 11211

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Marker-assisted screening of promising forms in the strawberry breeding

Cited by 2 publications

References 16 publications

Analysis of the inheritance of the marker SCAR-R1A, linked to the Rpf1 red stele root rot resistance gene, in strawberry hybrid progeny

Analysis of the inheritance of the marker SCAR-R1A, linked to the Rpf1 red stele root rot resistance gene, in strawberry hybrid progeny

Identification of <i>Rca2</i> and <i>Rpf1</i> resistance alleles in strawberry breeding forms of intervarietal hybrid origin

Contact Info

Product

Resources

About