Marker Aided Incorporation of Saltol, a Major QTL Associated with Seedling Stage Salt Tolerance, into Oryza sativa ‘Pusa Basmati 1121’

Babu, N. Naresh; Krishnan, S. Gopala; Vinod, K. K.; Krishnamurthy, S. L.; Singh, Vivek Kumar; Singh, Madan Pal; Singh, Renu; Ellur, Ranjith Kumar; Rai, Vandna; Bollinedi, Haritha; Bhowmick, Prolay Kumar; Yadav, Ashutosh Kumar; Nagarajan, M.; Singh, Nagendra; Prabhu, K. V.; Singh, Ashok Kumar

doi:10.3389/fpls.2017.00041

Cited by 67 publications

(55 citation statements)

References 73 publications

(123 reference statements)

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“…(Ellur et al 2016 ) Pusa 1734 – PB 1121/FL478/PB 1121*3 A MAS derived near-isogenic line of PB 1121 possessing “ Saltol ”, a major QTL governing tolerance to salt stress at seedling stage Not yet released. (Babu et al 2017 ) …”

Section: Important Varieties and Genotypes Developed Using Pb 1121 Asmentioning

confidence: 99%

Pusa Basmati 1121 – a rice variety with exceptional kernel elongation and volume expansion after cooking

Singh¹,

Singh

Mohapatra

et al. 2018

Rice

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Pusa Basmati 1121 (PB 1121) is a landmark Basmati rice variety having Basmati quality traits introgressed from traditional Basmati varieties such as Basmati 370 and Type 3. It was released for commercial cultivation in 2003. It possesses extra-long slender milled grains (9.00 mm), pleasant aroma, and an exceptionally high cooked kernel elongation ratio of 2.5 with a cooked kernel length of up to 22 mm, volume expansion more than four times, appealing taste, good mouth feel and easy digestibility. Owing to its exceptional quality characteristics, it has set new standards in the Basmati rice market. The cumulative foreign exchange earnings through export of PB 1121 since 2008 have been US$ 20.8 billion, which has brought prosperity to millions of Basmati farmers. During 2017, the farmers cultivating PB 1121 earned on an average US$ 1400/ha as against US$ 650/ha cultivating traditional Basmati, making it a highly profitable enterprise. Currently, PB 1121 is grown in ~ 70% of the total area under Basmati rice cultivation in India. It is the most common Basmati rice variety in rice grain quality research for developing mapping populations, genetic analyses and molecular mapping of Basmati quality traits. Additionally, it has been widely used in the Basmati rice breeding program across India, because of its superior quality attributes. This article presents an account of development of PB 1121, its major characteristic features and its flagship role in heralding a Basmati rice revolution. The prospective role of PB 1121 in Basmati rice improvement and future Basmati rice research as a whole is also presented.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0213-6) contains supplementary material, which is available to authorized users.

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Section: Important Varieties and Genotypes Developed Using Pb 1121 Asmentioning

confidence: 99%

Pusa Basmati 1121 – a rice variety with exceptional kernel elongation and volume expansion after cooking

Singh¹,

Singh

Mohapatra

et al. 2018

Rice

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“…9311: their seedling root and shoot growth was inhibited more strongly, and their leaves suffered a greater degree of chlorosis ( Figures 1A,B ). A key component of salinity tolerance relates to Na + and K + homeostasis ( Babu et al, 2017 ). The [K + ] of salinity-stressed cv.…”

Section: Discussionmentioning

confidence: 99%

Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

et al. 2018

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Salinity imposes a major constraint over the productivity of rice. A set of chromosome segment substitution lines (CSSLs), derived from a cross between the japonica type cultivar (cv.) Nipponbare (salinity sensitive) and the indica type cv. 9311 (moderately tolerant), was scored using a hydroponics system for their salinity tolerance at the seedling stage. Two of the CSSLs, which share a ∼1.2 Mbp stretch of chromosome 4 derived from cv. Nipponbare, were as sensitive to the stress as cv. Nipponbare itself. Fine mapping based on an F2 population bred from a backcross between one of these CSSLs and cv. 9311 narrowed this region to 95 Kbp, within which only one gene (OsHAK1) exhibited a differential (lower) transcript abundance in cv. Nipponbare and the two CSSLs compared to in cv. 9311. The gene was up-regulated by exposure to salinity stress both in the root and the shoot, while a knockout mutant proved to be more salinity sensitive than its wild type with respect to its growth at both the vegetative and reproductive stages. Seedlings over-expressing OsHAK1 were more tolerant than wild type, displaying a superior photosynthetic rate, a higher leaf chlorophyll content, an enhanced accumulation of proline and a reduced level of lipid peroxidation. At the transcriptome level, the over-expression of OsHAK1 stimulated a number of stress-responsive genes as well as four genes known to be involved in Na+ homeostasis and the salinity response (OsHKT1;5, OsSOS1, OsLti6a and OsLti6b). When the stress was applied at booting through to maturity, the OsHAK1 over-expressors out-yielded wild type by 25%, and no negative pleiotropic effects were expressed in plants gown under non-saline conditions. The level of expression of OsHAK1 was correlated with Na+/K+ homeostasis, which implies that the gene should be explored a target for molecular approaches to the improvement of salinity tolerance in rice.

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“…This modified MABB strategy proved to be highly successful and was then on utilised not only for recovering the grain quality but also for other agronomic traits as well. This is a standard protocol in MABB for recovery of the recurrent parent phenome and had been successfully employed in introgressing genes governing resistance to BB and blast diseases and also for QTLs governing salt tolerance in Basmati rice varieties (Singh et al 2012a;Ellur et al 2016a,b;Khanna et al 2015 a,b;Babu et al 2017;Singh et al 2018a;Sagar et al 2018).…”

Section: Factors That Enabled Successful Integration Of Mas In Rice Bmentioning

confidence: 99%

Precision breeding with genomic tools: A decade long journey of molecular breeding in rice

Singh¹,

Krishnan²,

Vinod³

et al. 2019

IJGPB

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Plant breeding helps in recombining and fixation of desirable alleles which enables the development of improved varieties with better productivity. Though classical plant breeding has contributed enormously in achieving genetic gains in different crops including rice, the challenges imposed by resource constraints as well as greater anticipated demand need better tools to meet the expectations. Molecular breeding which uses modern genomic tools integrated with plant breeding offers enormous potential for improvement of crop varieties. The present review focusses on the achievements of ICAR-IARI in molecular breeding in rice wherein as many as eight rice varieties were incorporated with genes governing resistance/tolerance to different traits and released for commercial cultivation in India. The various factors which enabled the successful integration of molecular breeding including development of infrastructure, human resources and the genetic and genomic resources have been discussed in detail. All the examples of translational research are from the marker assisted backcross breeding strategy, which limits the scope for achieving yield gains. In the foregoing discussion, the challenges and the opportunities for implementation of molecular breeding in rice to realize the full potential of these tools in plant breeding are discussed based on the decade long experience, which may be valuable for other crops as well.

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Marker Aided Incorporation of Saltol, a Major QTL Associated with Seedling Stage Salt Tolerance, into Oryza sativa ‘Pusa Basmati 1121’

Cited by 67 publications

References 73 publications

Pusa Basmati 1121 – a rice variety with exceptional kernel elongation and volume expansion after cooking

Pusa Basmati 1121 – a rice variety with exceptional kernel elongation and volume expansion after cooking

Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

Precision breeding with genomic tools: A decade long journey of molecular breeding in rice

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