Marinomonas profundi sp. nov., isolated from deep seawater of the Mariana Trench

Abstract

A Gram-stain-negative, aerobic, polarly flagellated, straight or curved rod-shaped bacterium, designated strain M1K-6T, was isolated from deep seawater samples collected from the Mariana Trench. The strain grew at −4 to 37 °C (optimum, 25–30 °C), at pH 5.5–10.0 (optimum, pH 7.0) and with 0.5–14.0 % (w/v) NaCl (optimum, 2.0 %). It did not reduce nitrate to nitrite nor hydrolyse gelatin or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M1K-6T was affiliated with the genus Marinomonas , sharing 93.1–97.0 % sequence similarity with the type strains of recognized Marinomonas species. The major cellular fatty acids were summed feature 3 (C16 : 1 ω6c/C16 : 1 ω7c), summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c), C16 : 0, C10 : 0 3-OH and C18 : 0. The predominant respiratory quinone was ubiquinone-8. Polar lipids of strain M1K-6T included phosphatidylethanolamine, phosphatidylglycerol and two unidentified lipids. The genomic G+C content of strain M1K-6T was 46.0 mol%. Based on data from the present polyphasic study, strain M1K-6T was considered to represent a novel species within the genus Marinomonas , for which the name Marinomonas profundi sp. nov. is proposed. The type strain is M1K-6T (=KCTC 72501T=MCCC 1K03890T).

Halomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench

“…Strains MT13 T and MT32 were isolated from a sediment sample collected using a gravity sampler at a depth of 8300 m at a site in the Mariana Trench (142.32° E, 11.12° N) during a cruise of R/V DongFang-Hong 2 in March 2019. Approximately 5 g sediment was added into a 100 ml Erlenmeyer flask containing 20 ml sterile artificial seawater (ASW; prepared with Sigma sea salts, 3%) and shaken at 180 r.p.m., 25…”

“…The Mariana Trench is located in the western Pacific Ocean, about 2550 km long and 70 km wide with a greatest depth of more than 11 km, representing the deepest part of the world's oceans. In spite of its harsh environments, mainly featuring high pressure and low temperature, the Mariana Trench contains huge and diverse microbes [21][22][23][24][25]. Isolating and identifying novel microbial strains from the Mariana Trench is the beginning of further exploration of environmental adaptation strategies and exploiting the application potential of these particular microbial resources.…”

Marinomonas lutimaris sp. nov., isolated from a tidal flat sediment of the East China Sea

“…Most species have been isolated from seawater [4][5][6][7][8][9][10][11][12] and marine sediments [13][14][15][16], as well as corals [17], seagrass [18], macroalgae [19,20] and reared clams (Ruditapes decussatus) [21]. The genus Marinomonas is widespread in diverse marine environments, including Antarctica [6,10], the Mariana and Yap Trenches [9,15], the Pacific Ocean [15], the Black Sea [22], the Arctic Ocean [6,10] and mangrove sediment [16]. In this study, we report the characterization of a novel bacterium of the genus Marinomonas isolated from a tidal flat sediment of the East China Sea.…”

“…To date, the genus Marinomonas comprises 36 species with validly published names (https://lpsn.dsmz.de/genus/marinomonas). Most species have been isolated from seawater [4–12] and marine sediments [13–16], as well as corals [17], seagrass [18], macroalgae [19, 20] and reared clams ( Ruditapes decussatus ) [21]. The genus Marinomonas is widespread in diverse marine environments, including Antarctica [6, 10], the Mariana and Yap Trenches [9, 15], the Pacific Ocean [15], the Black Sea [22], the Arctic Ocean [6, 10] and mangrove sediment [16].…”