Marinomonas pontica sp. nov., isolated from the Black Sea

Abstract

A Gram-negative, polarly flagellated bacterium was isolated from a sea-water sample collected from the Karadag Natural Reserve of the Eastern Crimea and characterized to clarify its taxonomic position. 16S rRNA gene sequence-based phylogenetic analysis of this novel organism revealed Marinomonas vaga, Marinomonas communis, Marinomonas mediterranea, Marinomonas primoryensis and 'Marinomonas protea' as its closest relatives (similarity 95-97 %). The G+C content of the DNA was 46?5 mol%. The organism grew between 4 and 33 6C, tolerated 10 % NaCl, was slightly alkaliphilic and was not able to degrade starch, gelatin, agar or Tween 80. Phosphatidylethanolamine (53?4 %) and phosphatidylglycerol (46?6 %) were the predominant phospholipids. The major fatty acids were 16 : 0 (15?5 %), 16 : 1v7 (26?7 %) and 18 : 1v7 (47?1 %). The phylogenetic, genetic and physiological properties of the organism placed it within a novel species, proposed as Marinomonas pontica sp. nov., the type strain of which is 46-16 T (=LMG 22531 T =KMM 3492 T =UCM 11075 T ).

Marinomonas mangrovi sp. nov., isolated from mangrove sediment

“…S3). The predominant phospholipids of strain B20-1 T (PE and PG) are in accordance with the data reported for Marinomonas pontica (Ivanova et al , 2005) and M. hwangdonensis (Jung et al , 2012).…”

Marinomonas profundi sp. nov., isolated from deep seawater of the Mariana Trench

“…The genus Marinomonas currently comprises 33 species with validly published names (https://lpsn.dsmz.de/genus/marinomonas) and represents a group of Gram-negative, strictly aerobic, polarly flagellated, straight or curved rod-shaped, heterotrophic bacteria having C 18 : 1 ω 7 c , C 16 : 1 ω 7 c , C 16 : 0 and C 10 : 0 3-OH as the characteristic fatty acids and DNA G+C content of 41–50 mol% [4, 5]. Almost all the species in this genus are of marine origin with habitats such as seawater [6–9], sea-ice [10], sediments [11, 12], seagrass [13, 14] and marine animals [15]. During an investigation of the microbial diversity in seawater samples collected from the Mariana Trench, a Marinomonas -like strain, designated M1K-6 T , was isolated from a 1045 m deep seawater sample.…”

“…ushuaiensis MCCC 1A00526 T and the type strains of other known Marinomonas species (Fig. S6) [5, 7, 9, 10, 14]. In addition, L1 was found commonly among strain M1K-6 T , M.…”

Marinomonas gallaica sp. nov. and Marinomonas atlantica sp. nov., isolated from reared clams (Ruditapes decussatus)

“…The genus Marinomonas, within the family Oceanospirillaceae, is composed of Gram-negative bacteria that are present in different marine environments such as the Mediterranean sea (Solano & Sanchez-Amat, 1999;Maci an et al, 2005), Black sea (Ivanova et al, 2005) and Arctic Ocean (Zhang et al, 2008). The genus was proposed by Van Landschoot & De Ley (1983) to accommodate two unusual species of the genus Alteromonas, Alteromonas communis and Alteromonas vaga.…”