Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment

Handley, Kim M.; Héry, Marina; Lloyd, Jonathan R.

doi:10.1099/ijs.0.003145-0

Cited by 70 publications

(58 citation statements)

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“…In addition, the reports of an arsenate-respiring and arsenite-oxidizing marine species, Marinobacter santoriniensis sp. isolated from hydrothermal sediments (Handley et al 2009), allow us to infer that Marinobacter-related sequences could be also associated to arsenic-metabolizing microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Enrichment of arsenic transforming and resistant heterotrophic bacteria from sediments of two salt lakes in Northern Chile

et al. 2012

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Microbial populations are involved in the arsenic biogeochemical cycle in catalyzing arsenic transformations and playing indirect roles. To investigate which ecotypes among the diverse microbial communities could have a role in cycling arsenic in salt lakes in Northern Chile and to obtain clues to facilitate their isolation in pure culture, sediment samples from Salar de Ascotán and Salar de Atacama were cultured in diluted LB medium amended with NaCl and arsenic, at different incubation conditions. The samples and the cultures were analyzed by nucleic acid extraction, fingerprinting analysis, and sequencing. Microbial reduction of As was evidenced in all the enrichments carried out in anaerobiosis. The results revealed that the incubation factors were more important for determining the microbial community structure than arsenic species and concentrations. The predominant microorganisms in enrichments from both sediments belonged to the Firmicutes and Proteobacteria phyla, but most of the bacterial ecotypes were confined to only one system. The occurrence of an active arsenic biogeochemical cycle was suggested in the system with the highest arsenic content that included populations compatible with microorganisms able to transform arsenic for energy conservation, accumulate arsenic, produce H(2), H(2)S and acetic acid (potential sources of electrons for arsenic reduction) and tolerate high arsenic levels.

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Section: Discussionmentioning

confidence: 99%

Enrichment of arsenic transforming and resistant heterotrophic bacteria from sediments of two salt lakes in Northern Chile

et al. 2012

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“…Indeed, certain members have been shown to harbor features favoring them for such environments: M. manganoxydans MnI7-9 was actually isolated from a metal-associated environment and was demonstrated to oxidize manganese (II) (Wang et al 2012). M. santoriniensis NKSG1 originated from metal-containing sediments of the Adriatic Sea and showed a nitrate dependent oxidation of arsenate (Handley et al 2009). A number of fully sequenced Marinobacter genomes is already available in public databases (e.g.…”

Section: Discussionmentioning

confidence: 97%

“…The potential to oxidize iron II was shown in Marinobacter hydrocarbonoclasticus VT8 (former Marinobacter aquaeolei) at the genomic level and experimentally (Singer et al 2011). A nitrate-dependent oxidation of arsenate was observed in Marinobacter santoriniensis NKSG1 (Handley et al 2009). Wang et al (2012) isolated Marinobacter manganoxydans MnI7-9 from a deep sea vent habitat enriched in heavy metals.…”

Section: Introductionmentioning

confidence: 95%

“…The genus Marinobacter comprises a heterogeneous group of more than 40 isolated c-proteobacterial species that are ubiquitously found in a wide range of marine habitats (Aguilera et al 2009;Gauthier et al 1992;Gaerdes et al 2010;Handley et al 2009;Handley and Lloyd 2013;Zhang et al 2008). Handley and Lloyd (2013) postulated a specification in habitats occupied by Marinobacter species.…”

Section: Introductionmentioning

confidence: 99%

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Marinobacter adhaerens HP15 harbors two CzcCBA efflux pumps involved in zinc detoxification

Stahl

Pletzer

Mehmood

et al. 2015

Antonie van Leeuwenhoek

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Several members of the ubiquitously found γ-proteobacterial genus Marinobacter were described or assumed to inhabit marine environments naturally enriched in heavy metals. However, direct studies that describe the ability of this genus to occupy such environments have not been conducted. To cope with heavy metal stress, bacteria possess specific efflux pumps as tools for detoxification, among which the CzcCBA type efflux system is one representative. Previous studies showed that this system plays an important role in resistance towards cadmium, zinc, and cobalt. Up to now, no study had focused on characterization of Czc pumps in Marinobacter sp. or other marine prokaryotes. Herein, we elucidated the function of two CzcCBA pumps encoded by Marinobacter adhaerens HP15's genome during exposure to cadmium, zinc, and cobalt. Single and double knock-out mutants lacking the corresponding two czcCBA operons were generated and analyzed in terms of their resistance profiles. Both operons appeared to be important for zinc resistance but had no role in tolerance towards cadmium or cobalt. One of the mutations was genetically complemented thereby restoring the wild type phenotype. In accordance with the resistance pattern, expression of the genes coding for both CzcCBA pumps was induced by zinc but neither by cadmium nor cobalt.

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“…At the time of writing, 34 species have been validly named (http://www. bacterio.net/marinobacter.html), which were isolated from diverse environments, including seawater (Gauthier et al 1992;Gorshkova et al 2003;Yoon et al 2003;2004;Romanenko et al 2005;Shivaji et al 2005;Antunes et al 2007;Roh et al 2008;Huo et al 2008;Zhang et al 2008;Xu et al 2008;Zhuang et al 2009;Kharroub et al 2011;Qu et al 2011;Lee et al 2012), marine sediment (Gorshkova et al 2003;Romanenko et al 2005;Kim et al 2006;Guo et al 2007; Montes et al 2008;Liu et al 2012;Gao et al 2013), saline lake (Aguilera et al 2009;Bagheri et al 2013), saltern (Yoon et al 2007;Wang et al 2009), saline soil (Martín et al 2003;), hot spring (Shieh et al 2003), hydrothermal sediment (Handley et al 2009), wastewater (Liebgott et al 2006) and others organisms (Romanenko et al 2005;Green et al 2006;Kaeppel et al 2012). The DNA G?C content of this genus ranges from 52.7 to 59.6 mol %.The predominant fatty acids are C 18:1 x9c, C 16:0 ,C 16:1 x9c and C 12:0 3-OH.…”

Section: Introductionmentioning

confidence: 99%

Marinobacter shengliensis sp. nov., a moderately halophilic bacterium isolated from oil-contaminated saline soil

Luo

Xie

et al. 2015

Antonie van Leeuwenhoek

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Two moderately halophilic strains, designated SL013A34A2(T) and SL013A24A, were isolated from oil-contaminated saline soil from Shengli Oilfield, eastern China. Cells were found to be Gram-staining negative, aerobic, rod-shaped with a single polar flagellum. The isolates were found to grow at 10-40 °C (optimum 35 °C), pH 6.0-9.0 (optimum pH 8.0), and NaCl concentrations of 0.5-18.0 % (w/v) (optimum 3.0-6.0 NaCl). The 16S rRNA gene sequence analysis indicated that the isolates belong to the genus Marinobacter. Strain SL013A34A2(T) shares the highest 16S rRNA gene sequence similarities with strain SL013A24A (99.3 %), followed by M. hydrocarbonoclasticus CGMCC 1.7683(T) (97.8 %), M. vinifirmus CGMCC 1.7265(T) (97.8 %), and M. excellens KMM 3809(T) (97.4 %), respectively, but low similarities (93.8-96.4 %) with type strains of the other numbers of genus Marinobacter. DNA-DNA relatedness values of strain SL013A34A2(T) with strains SL013A24A, M. hydrocarbonoclasticus CGMCC 1.7683(T), M. vinifirmus CGMCC 1.7265(T) and M. excellens KMM 3809(T) were 88.7, 29.2, 33.4 and 29.4 %, respectively. The major fatty acids of strain SL013A34A2(T) were identified as C18:1 ω9c, C16:0, C12:03-OH, C12:0, C16:1 ω9c and 10-methyl C18:0. The major respiratory quinone of strain SL013A34A2(T) was found to be ubiquinone-9, and its predominant polar lipids were identified as diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and unidentified glycolipid. The genomic DNA G + C content was found to be 56.1 mol %. Based on the phenotypic, genetic and chemotaxonomic characteristics, these two isolates are representatives of a novel species of the genus Marinobacter, for which the name Marinobacter shengliensis sp. nov. is proposed. The type strain is SL013A34A2(T)(=LMG 27740(T) = CGMCC 1.12758(T)).

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Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment

Cited by 70 publications

References 50 publications

Enrichment of arsenic transforming and resistant heterotrophic bacteria from sediments of two salt lakes in Northern Chile

Enrichment of arsenic transforming and resistant heterotrophic bacteria from sediments of two salt lakes in Northern Chile

Marinobacter adhaerens HP15 harbors two CzcCBA efflux pumps involved in zinc detoxification

Marinobacter shengliensis sp. nov., a moderately halophilic bacterium isolated from oil-contaminated saline soil

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