Marinobacter antarcticus sp. nov., a halotolerant bacterium isolated from Antarctic intertidal sandy sediment

Abstract

A Gram-staining-negative, aerobic, motile, oxidase-and catalase-positive, rod-shaped strain, designated ZS2-30 T , was isolated from Antarctic intertidal sandy sediment. The strain grew at 4-35 6C (optimum, 25 6C) and in 0-25 % (w/v) NaCl (optimum, 3.0-4.0 %). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30 T were summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c), C 16 : 0 , C 18 : 1 v9c, C 16 : 1 v9c, C 12 : 0 3-OH and C 12 : 0 . The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30 T was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30 T was affiliated with the genus Marinobacter. It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus Marinobacter, namely Marinobacter maritimus (98.3 %), Marinobacter psychrophilus (98.1 %) and Marinobacter goseongensis (97.1 %), but the DNA-DNA relatedness values between strain ZS2-30 T and the above three species were all lower than 45 %. Moreover, strain ZS2-30 T could be distinguished from closely related species of the genus Marinobacter by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30 T is considered to represent a novel species in the genus Marinobacter, for which the name Marinobacter antarcticus sp. nov. is proposed. The type strain of Marinobacter antarcticus is ZS2-30 T (5CGMCC 1.10835 T 5KCTC 23684 T ).The genus Marinobacter in the class Gammaproteobacteria was originally proposed by Gauthier et al. (1992) for a Gram-negative, extremely halotolerant, hydrocarbondegrading, rod-shaped bacterium isolated from Mediterranean seawater near a petroleum refinery. At the time of writing, the genus comprised 29 species (Gauthier et al.,

Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake

“…Enzyme activities detected with the API ZYM system and utilization of carbon sources by strain XCD-X12 T and the reference type strains are detailed in . Almost all the results obtained in this study for the reference type strains were consistent with the original descriptions (Gauthier et al , 1992; Liebgott et al , 2006; Liu et al , 2012), except for the activities of valine arylamidase and acid phosphatase for M. vinifirmus CGMCC 1.7265 T , suggesting that these properties may be variable in M. vinifirmus . Morphological, physiological and biochemical characteristics of strain XCD-X12 T are given in and the species description.…”

“…In addition, members of this genus have been reported to be marine polycyclic aromatic hydrocarbon degraders (Cui et al , 2008; Dastgheib et al , 2012; Melcher et al , 2002). At the time of writing, the genus Marinobacter consists of 34 species (http://www.bacterio.net/marinobacter.html) from various environments including salt lakes (Aguilera et al , 2009; Bagheri et al , 2013), saline soil (Gu et al , 2007; Martín et al , 2003), salterns (Qu et al , 2011; Wang et al , 2009; Yoon et al , 2007), brine samples of a salt concentrator (Kharroub et al , 2011), seawater (Antunes et al , 2007; Gauthier et al , 1992; Kaeppel et al , 2012; Roh et al , 2008; Shivaji et al , 2005; Xu et al , 2008; Yoon et al , 2003, 2004; Zhuang et al , 2009), sea sediment (Gao et al , 2013; Gorshkova et al , 2003; Guo et al , 2007; Huo et al , 2008; Montes et al , 2008; Romanenko et al , 2005), sea sand (Kim et al , 2006), marine animals (Green et al , 2006; Lee et al , 2012), coastal hydrothermal sediment (Handley et al , 2009), coastal hot springs (Shieh et al , 2003), wastewater (Liebgott et al , 2006), an oil-producing well (Huu et al , 1999), Arctic sea-ice (Zhang et al , 2008) and sandy sediment of the Antarctic (Liu et al , 2012). The diverse origins of members of the genus suggest that they are widely distributed and may play important roles in the environment, such as the degradation of aromatic hydrocarbons.…”

Marinobacter aromaticivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment

“…T and the type strains of the four closely related species were similar, consistent with a previous report (Chang Liu et al 2012). The main fatty acids of strain D15-8P T were C 12 : 0 , C 14 : 0 3-methyl, C 16 : 0 , C 16 : 1 v9c and C 18 : 1 v9c, and these fatty acids constitute 82.32 % of the entire fatty acids of this strain (Table S1).…”

Isolation, characterization and optimization of EPSs produced by a cold-adaptedMarinobacterisolate from Antarctic seawater

“…Cold-adapted Marinobacter from Antarctica have been poorly reported, although two novel halotolerant species from Antarctic sediments, i.e. Marinobacter antarcticus Liu et al (Liu et al 2012) and Marinobacter guineae Montes et al (Montes et al 2008), and brines (Chua et al 2018) have been described. Further, at the time of writing, no report exists on EPS-producing cold-adapted Marinobacter isolates.…”