Marine Extremophiles: A Source of Hydrolases for Biotechnological Applications

Dalmaso, G.; Ferreira, Davis Fernandes; Vermelho, Alane Beatriz

doi:10.3390/md13041925

Cited by 216 publications

(97 citation statements)

References 270 publications

(296 reference statements)

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“…Marine bacteria from this group are already known for the production of different antibiotics (Radjasa et al, 2007). Previous studies have also reported production of different hydrolases from marine bacteria belonging to genera Erwinia, Vibrio, Psychrobacter marinobacter, Chromohalobacter, Halomonas, Microbulbifer, and Alteromonas (Dalmaso et al, 2015), whereas the production of enzymes by marine Aidingimonas have not been previously reported. The second dominant phylum Firmicutes comprised of Bacillus, which has been reported as a dominant genus among all the marine enzyme-producing bacteria (Divya et al, 2010).…”

Section: Discussionmentioning

confidence: 98%

Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia

Bibi¹,

Ullah²,

Alvi

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Marine bacteria have been exceptional sources of halotolerant enzymes since decades. The aim of the present study was to isolate bacteria producing hydrolytic enzymes from seven different mangroves collected from the coastal area of Thuwal, Jeddah, Saudi Arabia, and to further screen them for other enzymatic and antifungal activities. We have isolated 46 different rhizo-and endophytic bacteria from the soil, roots, and leaves of the mangroves using different enzymatic media. These bacterial strains were capable of producing industrially important enzymes (cellulase, protease, lipase, and amylase). The bacteria were screened further for antagonistic activity against fungal pathogens. Finally, these bacterial strains were identified on the basis of the16S rDNA sequence. Taxonomic and phylogenetic analysis revealed 95.9-100% sequence identity to type strains of related species. The dominant phylum was Gammaproteobacteria (γ-Proteobacteria), which comprised 10 different genera -Erwinia, Vibrio, Psychrobacter, Aidingimonas, Marinobacter, Chromohalobacter, Halomonas, Microbulbifer, and Alteromonas. Firmicutes was the second dominant phylum, which contained only the genus Bacillus. Similarly, only Isoptericola belonged to Actinobacteria. Further these enzymeproducing bacteria were tested for the production of other enzymes. Most of the active strains showed cellulytic and lipolytic activities. Several were also active against fungal pathogens. Our results demonstrated that the mangroves represent an important source of potentially active bacteria producing enzymes and antifungal metabolites (bioactive products). These bacteria are a source of novel halophilic enzymes and antibiotics that can find industrial and medicinal use.

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Section: Discussionmentioning

confidence: 98%

Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia

Bibi¹,

Ullah²,

Alvi

et al. 2017

Genet. Mol. Res.

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“…These properties of the cold-adapted enzymes have resulted in their commercial use as for example in the biomedical and food industries[1, 2,[10][11][12][13][14][15]. The high catalytic efficiency of Atlantic cod trypsin is especially useful in the processing of biological materials.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of different cold-adapted Atlantic cod ( Gadus morhua ) trypsin X isoenzymes

Stefansson¹,

Sandholt

Guðmundsdóttir

2017

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Trypsins from Atlantic cod (Gadus morhua), consisting of several isoenzymes, are highly active cold-adapted serine proteases. These trypsins are isolated for biomedical use in an eco-friendly manner from underutilized seafood by-products. Our group has explored the biochemical properties of trypsins and their high potential in biomedicine. For broader utilization of cod trypsins, further characterization of biochemical properties of the individual cod trypsin isoenzymes is of importance. For that purpose, a benzamidine purified trypsin isolate from Atlantic cod was analyzed. Anion exchange chromatography revealed eight peaks containing proteins around 24 kDa with tryptic activity. Based on mass spectrometric analysis, one isoenzyme gave the best match to cod trypsin I and six isoenzymes gave the best match to cod trypsin X. Amino terminal sequencing of two of these six trypsin isoenzymes showed identity to cod trypsin X. Three sequence variants of trypsin X were identified by cDNA analysis demonstrating that various forms of this enzyme exist.One trypsin X isoenzyme was selected for further characterization based on abundance and stability. Stepwise increase in catalytic efficiency (k cat /K m ) of this trypsin X isoenzyme was obtained with substrates containing one to three amino acid residues. The study demonstrates that the catalytic efficiency of this trypsin X isoenzyme is comparable to that of cod trypsin I, the most abundant and highly active isoenzyme in the benzamidine cod trypsin isolate. Differences in pH stability and sensitivity to inhibitors of the trypsin X isoenzyme compared to cod trypsin I were detected that may be important for practical use.

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“…N8 was a good producer of protease and amylase, which were according to the findings of Liu et al (2007). The understanding of specific factors that confer the ability to withstand extreme habitats on such enzymes has become a priority for their biotechnological use (Dalmaso et al, 2015). It is important to study and understand these microorganisms in order to be able to use the biochemical, ecological, evolutionary and industrial potential of these endoliths and epiliths microbes.…”

Section: Discussionmentioning

confidence: 99%

Morphological, Biochemical and Molecular Characterization of Culturable Epilithic and Endolithic Bacteria From Rocks of Ayubia (Murree), Lower Himalaya, Pakistan

Khan¹,

Sajjad²,

Zada³

et al. 2018

Appl. Ecol. Env. Res.

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Abstract. Murree and Ayubia hills are parts of the lower Himalaya Mountain range in Pakistan. The rocks of these hills are younger and mostly limestone in nature. In this study, geomicrobiological investigation of the selected samples collected from Murree and Ayubia hills was carried out. The rock samples were spread on nutrient agar plates and endolithic and epilithic bacteria were isolated on the basis of colony morphology and studied further. All the isolates were screened for different industrially important hydrolytic enzymes. Study isolates were identified through 16S rRNA gene sequencing. These endoliths and epiliths were assayed for antimicrobial activities against clinical isolates S. aureus, P. aeuroginosa and E. coli. Total 31 bacterial isolates were recovered of which 15 were endolithic and 16 were epilithic. 16S rRNA gene sequencing revealed major culturable groups colonizing these rocks were clustered in four major groups, Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes which inlcude Alcaligenes, Lysinibacillus, Actinobacteria, Pseudomonas, Pusillimonas, Streptomyces, Fluviicola, Serratia, Flavobacterium, Stenotrophomonas and Brevindomonas species. The sequences were deposited in NCBI for acquisition of accession numbers. The bacterial isolates were efficient producers of oxidase, catalase, protease, amylase and gelatinase. The endolithic isolates N4 (Pseudomonas sp. KT223616) and N28 (Streptomyces sp. KT004386) exhibited good activity against all the three clinically isolated target strains. The study revealed the rocks of Murree and Ayubia hills have a rich microbial ecology that besides having an important role in weathering and mineralization processes may also be potential source of biotechnological applications.

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Marine Extremophiles: A Source of Hydrolases for Biotechnological Applications

Cited by 216 publications

References 270 publications

Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia

Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia

Elucidation of different cold-adapted Atlantic cod ( Gadus morhua ) trypsin X isoenzymes

Morphological, Biochemical and Molecular Characterization of Culturable Epilithic and Endolithic Bacteria From Rocks of Ayubia (Murree), Lower Himalaya, Pakistan

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