Marine Biosurfactants, II. Production and Characterization of an Anionic Trehalose Tetraester from the Marine Bacterium Arthrobacter sp. EK 1

Passeri, A.; Lang, Siegmund; Wagner, Fritz; Wray, Victor

doi:10.1515/znc-1991-3-408

Cited by 29 publications

(13 citation statements)

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“…strain EK1. In this tetraester, trehalose was found to be esterified at C-2 position with succinate (Passeri et al, 1991). R. erythropolis strain SD74 grown on n-hexadecane excreted two other trehalose tetraesters, 2,3,4,29-di-O-succinoyl-di-O-alkanoyl-a,atrehalose and 2,3,4-mono-O-succinoyl-di-O-alkanoyl-a,atrehalose.…”

Section: Discussionmentioning

confidence: 99%

Degradation of alkanes and highly chlorinated benzenes, and production of biosurfactants, by a psychrophilic Rhodococcus sp. and genetic characterization of its chlorobenzene dioxygenase

Rapp

Gabriel-Jürgens

2003

Microbiology

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Rhodococcus sp. strain MS11 was isolated from a mixed culture. It displays a diverse range of metabolic capabilities. During growth on 1,2,4-trichlorobenzene, 1,2,4,5-tetrachlorobenzene (1,2,4,5-TeCB) and 3-chlorobenzoate stoichiometric amounts of chloride were released. It also utilized all three isomeric dichlorobenzenes and 1,2,3-trichlorobenzene as the sole carbon and energy source. Furthermore, the bacterium grew well on a great number of n-alkanes ranging from n-heptane to n-triacontane and on the branched alkane 2,6,10,14-tetramethylpentadecane (pristane) and slowly on n-hexane and n-pentatriacontane. It was able to grow at temperatures from 5 to 30 degrees C, with optimal growth at 20 degrees C, and could tolerate 6 % NaCl in mineral salts medium. Genes encoding the initial chlorobenzene dioxygenase were detected by using a primer pair that was designed against the alpha-subunit (TecA1) of the chlorobenzene dioxygenase of Ralstonia (formerly Burkholderia) sp. strain PS12. The amino acid sequence of the amplified part of the alpha-subunit of the chlorobenzene dioxygenase of Rhodococcus sp. strain MS11 showed >99 % identity to the alpha-subunit of the chlorobenzene dioxygenase from Ralstonia sp. strain PS12 and the parts of both alpha-subunits responsible for substrate specificity were identical. The subsequent enzymes dihydrodiol dehydrogenase and chlorocatechol 1,2-dioxygenase were induced in cells grown on 1,2,4,5-TeCB. During cultivation on medium-chain-length n-alkanes ranging from n-decane to n-heptadecane, including 1-hexadecene, and on the branched alkane pristane, strain MS11 produced biosurfactants lowering the surface tension of the cultures from 72 to

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Section: Discussionmentioning

confidence: 99%

Degradation of alkanes and highly chlorinated benzenes, and production of biosurfactants, by a psychrophilic Rhodococcus sp. and genetic characterization of its chlorobenzene dioxygenase

Rapp

Gabriel-Jürgens

2003

Microbiology

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“…Thus, it has been found that indeed, RLs promote the uptake and biodegradation of poorly soluble substrates. Although most of the literature is concerned with the conversion of hydrocarbons and vegetable oils as substrates for production (Syldatk et al 1985;Passeri et al 1991;Lang and Wullbrandt 1999) water-miscible substrates, such as glucose or other carbohydrates have also been used (GuerraSantos et al 1984;Spoecker et al 1999). The production of extracellular RLs by P. aeruginosa is strictly regulated and maximal RLs synthesis is found under conditions of nitrogen limitation during the stationary phase of growth, using glucose, glycerol, or n-paraffins as carbon sources (Mulligan and Gibbs 1989).…”

Section: Introductionmentioning

confidence: 99%

On the Thermus thermophilus HB8 potential pathogenicity triggered from rhamnolipids secretion: morphological alterations and cytotoxicity induced on fibroblastic cell line

Pantazaki

Choli‐Papadopoulou

2011

Amino Acids

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A limited number of bacterial strains usually grown under nutrient limitation secrete rhamnolipids (RLs), which are recorded as virulence factors that are implicated in the pathogenicity of a microorganism. The non-pathogenic T. thermophilus HB8 produces extracellular rhamnolipids (TthRLs) under defined cultivation conditions using sunflower seed oil and sodium gluconate as carbon sources. In particular, the secreted TthRLs have been isolated, purified and identified with ATR-FTIR. Their effects on the cells' viability were examined when they were supplemented in a culture of human skin fibroblasts. Purified TthRLs triggered a sequence of rapid and pronounced morphological alterations characterized by transformation of fibroblast shape from polygonal to fusiform; retraction with cytoplasm condensation, rounding up, distortion of nuclei and loss of lamellar processes, and finally disruption of membrane. The addition of TthRLs in the cultured fibroblasts caused cytotoxicity, in contrast to that of rhamnose that stimulated viability, as it was assessed by MTT test. These results revealed that among the constituents of RLs that are implicated in the cytotoxicity, it has to be attributed to the lipidic chain variation and not to the carbohydrate part. TthRLs cytotoxicity on fibroblasts is comparable, and provoked similar effects, to that caused by saponin white, a known surfactant. TthRLs secretion might be a crucial point for the transformation of a non-pathogenic bacterium to a pathogenic one under certain environmental conditions favoring their secretion. RLs secretion in the microorganism's world might be a general route for the passage in the pathogenicity to ensure their survival under nutrient limitation conditions.

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“…However, biosurfactants are not only produced by micro‐organisms growing in water‐immiscible substrates, although most of the literature is concerned with the conversion of hydrocarbons and vegetable oils as substrates for production ( Syldatk et al . 1984 ; Passeri et al . 1991 ; Lang & Wullbrandt 1999).…”

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confidence: 99%

Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2 NCIB 40044 from waste frying oils

et al. 2000

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World production of oils and fats is about 2·5 million tonnes, 75% of which are derived from plants. Most of them are used in the food industry for the manufacture of different products, or directly as salad oil. Great quantities of waste are generated by the oil and fat industries: residual oils, tallow, marine oils, soap stock, frying oils. It is well known that the disposal of wastes is a growing problem and new alternatives for the use of fatty wastes should be studied. Used frying oils, due to their composition, have great potential for microbial growth and transformation. The use of economic substrates such as hydrophobic wastes meets one of the requirements for a competitive process for biosurfactant production. In the Mediterranean countries, the most used vegetable oils are sunflower and olive oil. Here we present a screening process is described for the selection of micro‐organism strains with the capacity to grow on these frying oils and accumulate surface‐active compounds in the culture media. From the 36 strains screened, nine Pseudomonas strains decreased the surface tension of the medium to 34–36 mN/M; the emulsions with kerosene remained stable for three months. Two Bacillus strains accumulated lipopeptide and decreased the surface tension to 32–34 mN/m. Strain Ps. aeruginosa 47T2 was selected for further studies. The effect of nitrogen and a C/N of 8·0 gave a final production of rhamnolipid of 2·7 g l−1 as rhamnose, and a production yield of 0·34 g g−1.

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Marine Biosurfactants, II. Production and Characterization of an Anionic Trehalose Tetraester from the Marine Bacterium Arthrobacter sp. EK 1

Cited by 29 publications

References 0 publications

Degradation of alkanes and highly chlorinated benzenes, and production of biosurfactants, by a psychrophilic Rhodococcus sp. and genetic characterization of its chlorobenzene dioxygenase

Degradation of alkanes and highly chlorinated benzenes, and production of biosurfactants, by a psychrophilic Rhodococcus sp. and genetic characterization of its chlorobenzene dioxygenase

On the Thermus thermophilus HB8 potential pathogenicity triggered from rhamnolipids secretion: morphological alterations and cytotoxicity induced on fibroblastic cell line

Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2 NCIB 40044 from waste frying oils

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