Marine actinomycetes related to the ‘<i>Salinospora</i>’ group from the Great Barrier Reef sponge <i>Pseudoceratina clavata</i>

Kim, Tae Kyung; Garson, Mary J.; Fuerst, John A.

doi:10.1111/j.1462-2920.2005.00716.x

Cited by 123 publications

(99 citation statements)

References 25 publications

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“…Improvements in this process should greatly facilitate passage of these compounds through the various stages of the drug-testing process. Actinomycete-selective media were also used successfully with the sponges Pseudoceratina clavata (188), Xestospongia spp. (235), Hymeniacidon perlevis (498), and Craniella australiensis (209).…”

Section: Methods For Accessing the Hidden Chemistry Of Marine Spongesmentioning

confidence: 99%

“…A PKS consistent with the biosynthesis of discodermolide was also not found (342). Direct amplification of KS domains was also combined with the construction of fosmid libraries to study PKSs in another sponge, the Great Barrier Reef species Pseudoceratina clavata (187,188). Each approach led to the retrieval of five KS domains, all of which fell into an apparently sponge-specific KS cluster (together with sequences obtained from Discodermia dissoluta, Theonella swinhoei, and an unidentified sponge) following phylogenetic analysis (187).…”

Section: Methods For Accessing the Hidden Chemistry Of Marine Spongesmentioning

confidence: 99%

“…The most frequently recovered sequences in general 16S rRNA gene surveys of sponges include those from the Acidobacteria, Actinobacteria, and Chloroflexi (148). Members of several bacterial phyla, namely, the Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Planctomycetes, Proteobacteria, and Verrucomicrobia, have also been isolated in pure culture from marine sponges (46,47,56,81,95,147,187,188,198,202,214,235,263,264,292,334,341,365,453,458). Sequences from the Chlorobi (green sulfur bacteria) have not been obtained from sponges, although positive fluorescence in situ hybridization (FISH) signals were obtained from Rhopaloeides odorabile with a specific probe for this phylum (458).…”

Section: Known Diversity Of Microorganisms From Spongesmentioning

confidence: 99%

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Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential

Taylor

Radax

Steger

et al. 2007

Microbiol Mol Biol Rev

1,273

1,512

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SUMMARY Marine sponges often contain diverse and abundant microbial communities, including bacteria, archaea, microalgae, and fungi. In some cases, these microbial associates comprise as much as 40% of the sponge volume and can contribute significantly to host metabolism (e.g., via photosynthesis or nitrogen fixation). We review in detail the diversity of microbes associated with sponges, including extensive 16S rRNA-based phylogenetic analyses which support the previously suggested existence of a sponge-specific microbiota. These analyses provide a suitable vantage point from which to consider the potential evolutionary and ecological ramifications of these widespread, sponge-specific microorganisms. Subsequently, we examine the ecology of sponge-microbe associations, including the establishment and maintenance of these sometimes intimate partnerships, the varied nature of the interactions (ranging from mutualism to host-pathogen relationships), and the broad-scale patterns of symbiont distribution. The ecological and evolutionary importance of sponge-microbe associations is mirrored by their enormous biotechnological potential: marine sponges are among the animal kingdom's most prolific producers of bioactive metabolites, and in at least some cases, the compounds are of microbial rather than sponge origin. We review the status of this important field, outlining the various approaches (e.g., cultivation, cell separation, and metagenomics) which have been employed to access the chemical wealth of sponge-microbe associations.

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Section: Methods For Accessing the Hidden Chemistry Of Marine Spongesmentioning

confidence: 99%

Section: Methods For Accessing the Hidden Chemistry Of Marine Spongesmentioning

confidence: 99%

Section: Known Diversity Of Microorganisms From Spongesmentioning

confidence: 99%

See 1 more Smart Citation

Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential

Taylor

Radax

Steger

et al. 2007

Microbiol Mol Biol Rev

1,273

1,512

View full text Add to dashboard Cite

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“…New actinobacteria with biopharmaceutical potential have been increasingly isolated from marine habitats (6,7,9,12).…”

mentioning

confidence: 99%

Discovery of a New Source of Rifamycin Antibiotics in Marine Sponge Actinobacteria by Phylogenetic Prediction

Kim

Hewavitharana

Shaw

et al. 2006

Appl Environ Microbiol

Self Cite

134

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Phylogenetic analysis of the ketosynthase (KS) gene sequences of marine sponge-derived Salinispora strains of actinobacteria indicated that the polyketide synthase (PKS) gene sequence most closely related to that of Salinispora was the rifamycin B synthase of Amycolatopsis mediterranei. This result was not expected from taxonomic species tree phylogenetics using 16S rRNA sequences. From the PKS sequence data generated from our sponge-derived Salinispora strains, we predicted that such strains might synthesize rifamycin-like compounds. Liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis was applied to one spongederived Salinispora strain to test the hypothesis of rifamycin synthesis. The analysis reported here demonstrates that this Salinispora isolate does produce compounds of the rifamycin class, including rifamycin B and rifamycin SV. A rifamycin-specific KS primer set was designed, and that primer set increased the number of rifamycin-positive strains detected by PCR screening relative to the number detectable using a conserved KS-specific set. Thus, the Salinispora group of actinobacteria represents a potential new source of rifamycins outside the genus Amycolatopsis and the first recorded source of rifamycins from marine bacteria.

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“…occur at abundances of up to 10 4 CFU/ml of sediment and can be isolated from worldwide locations, including the Caribbean Sea, the Sea of Cortez, the Red Sea, the tropical Atlantic Ocean off The Bahamas, the tropical Pacific Ocean off Guam (13,19), and from a sponge collected from the Great Barrier reef in Australia (15). Despite significant effort, we have yet to cultivate Salinispora strains from temperate Pacific or Arctic sediments, suggesting that their distribution may be restricted to tropical and subtropical latitudes.…”

mentioning

confidence: 99%

Culture-Dependent and Culture-Independent Diversity within the Obligate Marine Actinomycete Genus Salinispora

Mincer

Fenical

Jensen

2005

Appl Environ Microbiol

121

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Salinispora is the first obligate marine genus within the order Actinomycetales and a productive source of biologically active secondary metabolites. Despite a worldwide, tropical or subtropical distribution in marine sediments, only two Salinispora species have thus far been cultivated, suggesting limited species-level diversity. To further explore Salinispora diversity and distributions, the phylogenetic diversity of more than 350 strains isolated from sediments collected around the Bahamas was examined, including strains cultured using new enrichment methods. A culture-independent method, using a Salinispora-specific seminested PCR technique, was used to detect Salinispora from environmental DNA and estimate diversity. Overall, the 16S rRNA gene sequence diversity of cultured strains agreed well with that detected in the environmental clone libraries. Despite extensive effort, no new species level diversity was detected, and 97% of the 105 strains examined by restriction fragment length polymorphism belonged to one phylotype (S. arenicola). New intraspecific diversity was detected in the libraries, including an abundant new phylotype that has yet to be cultured, and a new depth record of 1,100 m was established for the genus. PCR-introduced error, primarily from Taq polymerase, significantly increased clone library sequence diversity and, if not masked from the analyses, would have led to an overestimation of total diversity. An environmental DNA extraction method specific for vegetative cells provided evidence for active actinomycete growth in marine sediments while indicating that a majority of sediment samples contained predominantly Salinispora spores at concentrations that could not be detected in environmental clone libraries. Challenges involved with the direct sequence-based detection of spore-forming microorganisms in environmental samples are discussed.Bacteria belonging to the order Actinomycetales, commonly referred to as actinomycetes, perform significant biogeochemical roles in terrestrial soils and are highly valued for their unparalleled ability to produce biologically active secondary metabolites. These bacteria account for ca. 45% of the bioactive microbial metabolites discovered (3) and have played a central role in the development of the modern pharmaceutical industry. The immense biotechnological utility of actinomycetes has led to exhaustive surveys of cultivars from terrestrial habitats and an associated increase in the numbers of known compounds being rediscovered due to a high rate of redundancy in the strains isolated. Given recent advances in our understanding of marine actinomycetes (7, 13, 24; see also a recent issue of Antonie van Leeuwenhoek, vol. 87, 2005, de-voted to the subject), strains isolated from the marine environment represent a relatively unexplored frontier for the discovery of new actinomycete biodiversity and a resource for novel secondary metabolites.Recently, we reported the discovery of the first obligate marine actinomycete genus for which the name "Salinospora...

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Marine actinomycetes related to the ‘Salinospora’ group from the Great Barrier Reef sponge Pseudoceratina clavata

Cited by 123 publications

References 25 publications

Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential

Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential

Discovery of a New Source of Rifamycin Antibiotics in Marine Sponge Actinobacteria by Phylogenetic Prediction

Culture-Dependent and Culture-Independent Diversity within the Obligate Marine Actinomycete Genus Salinispora

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