MARCADORES DE PATOGENICIDADE EM Yersinia enterocolitica O: 3 ISOLADAS DE SUÍNOS DO RIO DE JANEIRO

Leal, Tereza Cristina Arcanjo; Leal, Nilma Cintra; Almeida, Alzira Maria Paiva de

doi:10.1590/s0100-736x1997000100004

Cited by 5 publications

(7 citation statements)

References 19 publications

(13 reference statements)

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“…This strain also displayed different behavior in relation to the 15 other strains. Surprisingly, this strain harbored several pathogenetic markers that were not found in the other 15 (Leal et al, 1997). On the other hand, one serotype O:6 human strain also fit the genotypic profile 1, confirming previous observations that human strains are also found in pigs.…”

Section: Discussionsupporting

confidence: 85%

RAPD-PCR typing of Yersinia enterocolitica (Enterobacteriaceae) O:3 serotype strains isolated from pigs and humans

Leal

Almeida

1999

Genet. Mol. Biol.

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Sixteen strains of Yersinia enterocolitica serotype O:3, isolated from apparently healthy pigs collected in Rio de Janeiro, and four human strains of serotypes O:4, O:5, O:6 and O:13 were analyzed by RAPD-PCR. The strains were grouped into five genotypic profiles according to the amplification patterns obtained with three random primers. Fifteen of the 16 pig strains had identical amplification patterns, which was named genotypic profile 1. The one different profile was named genotypic profile 2. Genotypic profile 1 was also exhibited by the O:6 human serotype strain. The O:4 and O:13 human serotype strains showed similar amplification profiles with two primers. However, the third primer induced a distinct profile in each strain. Therefore, these two strains were placed into genotypic profile 3 and 4, respectively. Each primer produced a completely different amplification profile in the O:5 human serotype strain; therefore, it was named genotypic profile 5. The presence or absence of plasmids in the strains studied did not affect the amplification results. These results show that genetic variations can exist within a serotype, and strains of different serotypes can exhibit the same amplification profile when compared using other primers.

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Section: Discussionsupporting

confidence: 85%

RAPD-PCR typing of Yersinia enterocolitica (Enterobacteriaceae) O:3 serotype strains isolated from pigs and humans

Leal

Almeida

1999

Genet. Mol. Biol.

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“…Another hypothesis for the healthy condition of these animals is that they possess innate resistance to infection with Y. pseudotuberculosis (Hodges & Carman 1985). Leal et al (1997) phenotypically and genotypically characterizes some pathogenicity factors in Y. enterocolitica strains and observed the instability of some chromosomal segments in this bacterium. The authors attributed this instability to prolonged storage and manipulation after isolation, a fact indicating the importance for phenotypic and genotypic charcaterization of strains immediately after isolation.…”

Section: Discussionmentioning

confidence: 99%

Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Martins

Bauab

Leite

et al. 2007

Mem. Inst. Oswaldo Cruz

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“…The gene irp2 was included in this study as a chromosomal pathogenicity marker (Buchrieser et al 1998). Amplification of the gene ymt located on pFra was performed according to Leal et al (1997b) with primers drawn by the program PrimerSelect (DNAstar, Inc.).…”

Section: Methodsmentioning

confidence: 99%

Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence

Leal-Balbino¹,

Leal²,

Lopes³

et al. 2004

Mem. Inst. Oswaldo Cruz

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Plasmid and chromosomal genes encode determinants of virulence for (Perry & Fetherston 1997, Parkhill et al. 2001, Deng et al. 2002. A 102-kb, unstable chromosomal area (locus pgm) is essential for Y. pestis virulence (Fetherston et al. 1992, Hinnebusch et al. 1996, Buchrieser et al. 1998.Typical strains of Y. pestis harbour three plasmids: pPst (9.5kb), encoding a plasminogen activator protease (Pla) (Sodeinde & Goguen, 1988; pFra (90kb), encoding a capsular protein Fraction 1 (F1) with antiphagocytic activities (Du et al. 2002) and murine toxin (Ymt), required for survival in the flea (Hinnebusch et al. 2002); pYV (70kb), encoding the Yop virulon which comprises both the Yop effectors proteins and the proteins necessary for injecting them into host cells. The Yop virulon enables the bacteria to survive and multiply in the lymphoid tissues of the host (Cornelis 2002). Several insertion sequences (IS100, IS200, IS285) present in the three plasmids favour recombination events and genetic plasticity (Filippov et al. 1995, Parkhill et al. 2001, Deng et al. 2002.However, atypical strains lacking some plasmids have been found in several foci around the world. On the other hand, strains containing extra DNA bands or additional cryptic plasmids have also been found (Filippov et al. 1990, Chu et al. 1998. In vitro, Y. pestis genome is very plastic and several changes have been described: emergence of additional DNA-bands, increasing of plasmid molecular mass, and integration of plasmids into the bacterial chromosome with or without loss of functions (Zsigray et al. 1985, Protsenko et al. 1991. Furthermore, the typical plasmids may be eliminated spontaneously at a high frequency during storage in the laboratory or through successive subcultures (Protsenko et al. 1991).The study of the plasmid content of Y. pestis isolates from the plague foci of Northeast Brazil, stored in the laboratory for several decades, showed that some of them displayed an atypical plasmid profile characterized by the absence of some plasmids or by the presence of extra DNA bands (Leal et al. 1997a, Leal & Almeida 1999, Cavalcanti et al. 2002. The absence of plasmids and the emergence of extra-DNA bands in the Brazilian isolates could also have been produced during storage or handling in the laboratory.To observe possible alterations in plasmids of the cultures in vitro and the impact of the alterations to their pathogenicity, three low subcultured and highly pathogenic Y. pestis isolates were submitted to serial subcultures. Colonies selected after subcultures were analyzed for their plasmid content and the presence of some characteristic genes of each plasmid. LD 50 in mice of the parental strains and derived cultures displaying different plasmid contents were compared. MATERIALS AND METHODSBacteria and culture conditions -The study involved two Brazilian isolates: one old (P. Exu 340, originating from a finger bone marrow in a fatal human case in 1969), and the most recent Brazilian isolate (P. CE 882, originating from a hemoculture from a plagu...

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MARCADORES DE PATOGENICIDADE EM Yersinia enterocolitica O: 3 ISOLADAS DE SUÍNOS DO RIO DE JANEIRO

Cited by 5 publications

References 19 publications

RAPD-PCR typing of Yersinia enterocolitica (Enterobacteriaceae) O:3 serotype strains isolated from pigs and humans

RAPD-PCR typing of Yersinia enterocolitica (Enterobacteriaceae) O:3 serotype strains isolated from pigs and humans

Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence

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