Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro

Zhu, Shaihong; Aspera-Werz, Romina H.; Chen, Tao; Weng, Weidong; Braun, Bianca; Histing, Tina; Nüssler, Andreas K.

doi:10.17179/excli2020-3244

Cited by 8 publications

(13 citation statements)

References 52 publications

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“…As for NAC, cell viability showed a remarkable reduction when the concentration exceeded 7 mM ( Supplementary Figure S1e,f ). Previous studies also reported that 1.5 μg/mL MBE [ 17 ] and 50 μg/mL GE [ 48 ] did not have cytotoxic effects in vitro. Therefore, based on our results and the literature, 1.5 μg/mL MBE and 50 μg/mL GE were used for subsequent experiments.…”

Section: Resultsmentioning

confidence: 89%

“…Numerous studies have revealed that antioxidants can exert protective effects on bone homeostasis by inhibiting oxidative stress and, therefore, could be a beneficial supplement to support bone regeneration in smokers [ 17 , 18 , 19 ]. Maqui berry, a fruit native to South America, has been reported to have high antioxidant activity due to its rich content of anthocyanins and tea polyphenols [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…As a standard extract product, Delphinol ® maqui berry extract (MBE) contains 25% delphinidin (one of the anthocyanins) and is the richest natural source of delphinidins known to date [ 18 ]. Our previous study showed that MBE enhanced primary human osteoblast functions in CSE-induced oxidative stress damage via activation of the antioxidant defense system [ 17 ]. However, the effects of MBE on the interaction between osteoblasts and osteoclasts and their influence on osteoclast functions have not been investigated until now.…”

Section: Introductionmentioning

confidence: 99%

“…Under stress conditions, Nrf2 is liberated from the Keap1-Nrf2 complex and translocated to the nucleus, where it binds the antioxidant response element (ARE) sequences in the promoter regions on antioxidant genes [ 34 ]. Antioxidants, such as N-acetyl cysteine (NAC) and MBE, have been shown to protect bone-like cells against ROS and related cell injury by activating Nrf2 signaling [ 13 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model

et al. 2022

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Cigarette smoking-induced oxidative stress has harmful effects on bone metabolism. Maqui berry extract (MBE) and ginseng extract (GE) are two naturally occurring antioxidants that have been shown to reduce oxidative stress. By using an osteoblast and osteoclast three-dimensional co-culture system, we investigated the effects of MBE and GE on bone cells exposed to cigarette smoke extract (CSE). The cell viability and function of the co-culture system were measured on day 14. Markers of bone cell differentiation and oxidative stress were evaluated at gene and protein levels on day 7. The results showed that exposure to CSE induced osteoporotic-like alterations in the co-culture system, while 1.5 µg/mL MBE and 50 µg/mL GE improved CSE-impaired osteoblast function and decreased CSE-induced osteoclast function. The molecular mechanism of MBE and GE in preventing CSE-induced bone cell damage is linked with the inhibition of the NF-κB signaling pathway and the activation of the Nrf2 signaling pathway. Therefore, MBE and GE can reduce CSE-induced detrimental effects on bone cells and, thus, prevent smoking-induced alterations in bone cell homeostasis. These two antioxidants are thus suitable supplements to support bone regeneration in smokers.

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Section: Resultsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model

et al. 2022

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“…For this reason, most of the studies carried out on maqui have been focused on using fruit extracts because they exhibit pharmacological activities of high relevance, mainly associated with the content of anthocyanins [ 11 , 12 ]. The anthocyanin profile in maqui fruit, which is abundant in delphinidin derivatives, has been characterized by different researchers [ 10 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Metabolomic Profile and Antioxidant Content of Adult and In Vitro Leaves of Aristotelia chilensis

CRISÓSTOMO-AYALA

Sabater-Jara

Pérez

et al. 2021

Plants

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This work aimed to identify the bioactive compounds present in adult maqui (Aristotelia chilensis) leaves from different stages of development and seasons of the year and compare them with leaves obtained from maqui plants grown in vitro. The qualitative and quantitative analysis of maqui leaf extracts by HPLC-DAD-ESI-MSn showed the presence of different polyphenolic compounds classified into galloyl and caffeoyl quinic acids, ellagitannins and ellagic acid- and flavonoid-derivatives. In general, the total phenolic content of the in vitro samples was higher than that of ex vitro samples, whereas the total flavonoid content was higher in winter basal leaves. Additionally, the analysis by HPLC-MS showed that the extract from spring basal leaves was enriched in quercetin, catechin, kaempferol and 3-caffeoyl quinic acids, while in the in vitro leaves extract, quercetin was not present. As regards lipophilic compounds identified by GC/MS, the samples of in vitro leaves showed a high presence of α-tocopherol and β-sitosterol. In contrast, the samples of adult leaves presented a hight level of linolenic and linoleic acids. These results suggest that maqui leaves could be an excellent source of antioxidants and lipophilic compounds for many industries, such as the nutraceutical and pharmaceutical industries.

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In vitro modeling of liver fibrosis in 3D microtissues using scalable micropatterning system

et al. 2022

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Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro

Cited by 8 publications

References 52 publications

Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model

Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model

Comparative Study of Metabolomic Profile and Antioxidant Content of Adult and In Vitro Leaves of Aristotelia chilensis

In vitro modeling of liver fibrosis in 3D microtissues using scalable micropatterning system

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