Mapping the Subgroup Epitopes of Rotavirus Protein VP6

López, Susana; Espinosa, Rafaela; Greenberg, Harry B.; Arias, Carlos F.

doi:10.1006/viro.1994.1519

Cited by 47 publications

(35 citation statements)

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“…The results also confirmed the findings described by Lopez et al [1994] that an Ala residue at positions 172 and 305 is specific for SG I epitope and an Asn residue at 305 is specific for SG II epitope. Although 21 amino acids have been identified as SG I-or SG II-specific residues in our study and 20 amino acids by others [Tang et al, 1997], few amino acid positions have been demonstrated to contribute to the reactivity of SG-specific MAbs [Gorziglia et al, 1988;Lopez et al, 1994;Tang et al, 1997].…”

Section: Discussionsupporting

confidence: 91%

“…Although 21 amino acids have been identified as SG I-or SG II-specific residues in our study and 20 amino acids by others [Tang et al, 1997], few amino acid positions have been demonstrated to contribute to the reactivity of SG-specific MAbs [Gorziglia et al, 1988;Lopez et al, 1994;Tang et al, 1997]. Other amino acid residues located distantly in the VP6 protein and found to be conserved for SG I or SG II may also contribute to the formation of SG-specific epitopes by the folding of VP6 or interaction between VP6 monomers [Gorziglia et al, 1988].…”

Section: Discussioncontrasting

confidence: 44%

“…A number of studies have described the SG-specific amino acid residues for SG I and SG II on the VP6 protein of rotaviruses [Gorziglia et al, 1988;Lopez et al, 1994;Tang et al, 1997]. A total of 20 SG-specific amino acids have been identified and most studies have focused mainly on the VP6 region encompassing the amino acid positions previously recognized by SG-specific MAbs.…”

Section: Discussionmentioning

confidence: 99%

“…Region A (amino acids 45, 56) and region C (amino acids 114, 120) might contribute to the SG I epitope while regions B (amino acids 83,86,89,92), D (amino acids 312 or 314, 317 or 319) and E (amino acids 341 or 343, 350 or 352) might contribute to SG II epitope [Gorziglia et al, 1988]. Lopez et al [1994] have demonstrated that a single amino acid change at position 172 (Met to Ala) or 305 (Asn to Ala) is sufficient to change the SG specificity of human rotavirus Wa from SG II to SG (I þ II). These mutations allow the protein to be recognized by the SG I MAb 255/60, while retaining its capacity to interact with the SG II MAb 631/9.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities

Thongprachum

Khamrin

Saekhow

et al. 2008

Journal of Medical Virology

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Full-length VP6 amino acid sequences of human and porcine rotaviruses with subgroup (SG) (I þ II) and SG non-(I þ II) were analyzed in comparison with those of SG I and SG II. In human rotaviruses, the strains in the same SG shared a very high degree of amino acid identity, ranging from 97.4% to 99.4% for SG I, 95.9% to 100% for SG II, and 99.4% to100% for SG non-(I þ II), while viruses in different SGs shared somewhat lower sequence identity at 90.4-93.1%. Conserved amino acids that distinguished the strains of SG I from SG II were observed at 21 positions. The viruses with SG non-(I þ II) shared sequence identity with SG II as high as 97.2-99.7%, suggesting that they belonged to genogroup II. Similarly, porcine rotaviruses in the same SG shared 96.4-99.7% for SG I, 98.2-100% for SG II, 97.4-100% for SG (I þ II), and 96.2-99.7% for SG non-(I þ II), while strains in different SGs shared sequence identity ranging from 91.9% to 94.4%. Interestingly, the strains with SG (I þ II) and SG non-(I þ II) shared a high degree of sequence identity with SG I, at 96.4-100% and 94.7-99.7% respectively, suggesting that they are related to porcine SG I strains. The conserved amino acids which distinguished SG I from SG II were observed at 13 positions. The strains with SG I, SG (I þ II), and SG non-(I þ II) showed identical amino acid residues at these positions. Phylogenetic analysis strongly supported the findings of the sequence analysis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 44%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities

Thongprachum

Khamrin

Saekhow

et al. 2008

Journal of Medical Virology

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“…The SG specificities are determined by amino acids located at positions 305, the region between amino acids positions 296 and 299 (SGI) and amino acid position 315 (SGII) (López et al 1994, Tang et al 1997. Many epidemiological studies have used subgrouping enzyme immunoassays (EIAs).…”

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confidence: 99%

Group A rotavirus genotypes and the ongoing Brazilian experience: a review

Leite

Carvalho-Costa

Linhares

2008

Mem. Inst. Oswaldo Cruz

102

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Brazil was the first Latin American country to introduce universal group A rotavirus (RV-A) vaccination in March 2006, resulting in a unique epidemiological scenario. Since RV-A first identification in Brazil, 2,691 RV-A-positive stool samples, collected between 1982- 2007, were typed by independent research groups throughout the country. In the pre-vaccination era, 2,492 RV-A-positive samples collected from 1982-2005 were successfully typed, while 199 samples were analyzed from 2006-2007. According to the reviewed studies, there were two important times in the pre-vaccination era: (i) the period from 1982-1995, during which the detection of G5P[8] RV-A, in addition to the classical genotypes G1-4, challenged vaccine development programs; and (ii) the period from 1996-2005, during which genotype G9P[8] emerged, following a global trend. The rate of G2P[4] RV-A detection decreased from 26% (173/653) during 1982-1995 to 2% (43/1,839) during 1996-2005. The overall detection rate of RV-A genotypes from 1982-2005 was as follows: 43% (n = 1,079) G1P[8]/G1P[not typed (NT)]; 20% (n = 488) G9P[8]/G9P[NT]; 9% (n = 216) G2P[4]/G2P[NT]; 6% (n = 151) G3P[8]/G3P[NT]; 4% (n = 103) G4P[8]/G4P[NT]; and 4% (n = 94) G5P[8]/G5P[NT]. Mixed infections accounted for 189 (7%) of the positive samples, while atypical G/P combinations or other genotypes, including G6, G8, G10 and G12, were identified in 172 (7%) samples. The initial surveillance studies carried out in several Brazilian states with RV-A-positive samples collected in 2006 and 2007 show a predominance of G2P[4] strains (148/199 or 74%). Herein, we review RV-A typing studies carried out since the 1980s in Brazil, highlighting the dynamics of RV-A strain circulation profiles before and early after universal use of RV-A vaccine in Brazil

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Molecular characterization of a rare G3P[3] human rotavirus reassortant strain reveals evidence for multiple human-animal interspecies transmissions

et al. 2006

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An unusual strain of human rotavirus G3P[3] (CMH222), bearing simian-like VP7 and caprine-like VP4 genes, was isolated from a 2-year-old child patient during the epidemiological survey of rotavirus in Chiang Mai, Thailand in 2000-2001. The rotavirus strain was characterized by molecular analysis of its VP4, VP6, VP7, and NSP4 gene segments. The VP4 sequence of CMH222 shared the greatest homology with those of caprine P[3] (GRV strain) at 90.6% nucleotide and 96.4% amino acid sequence identities. Interestingly, the VP7 sequence revealed highest identity with those of simian G3 rotavirus (RRV strain) at 88% nucleotide and 98.1% amino acid sequence identities. In contrast, percent sequence identities of both the VP4 and VP7 genes were lower when compared with those of human rotavirus G3P[3] reference strains (Ro1845 and HCR3). Analyses of VP6 and NSP4 sequences showed a close relationship with simian VP6 SG I and caprine NSP4 genotype C, respectively. Phylogenetic analysis of VP4, VP6, VP7, and NSP4 genes of CMH222 revealed a common evolutionary lineage with simian and caprine rotavirus strains. These findings strongly suggest multiple interspecies transmission events of rotavirus strains among caprine, simian, and human in nature and provide convincing evidence that evolution of human rotaviruses is tightly intermingled with the evolution of animal rotaviruses.

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Mapping the Subgroup Epitopes of Rotavirus Protein VP6

Cited by 47 publications

References 0 publications

Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities

Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities

Group A rotavirus genotypes and the ongoing Brazilian experience: a review

Molecular characterization of a rare G3P[3] human rotavirus reassortant strain reveals evidence for multiple human-animal interspecies transmissions

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