Mapping the Sheep Genome: Production of Characterized Sheep X Hamster Cell Hybrids

Burkin, Dean J.; Morse, Helvise G.; Broad, T. E.; Pearce, P. D.; Ansari, H. A.; Lewis, P. E.; Jones, C. Hal

doi:10.1006/geno.1993.1212

Cited by 28 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…marker isolation strategy used for the construction of the sheep linkage map (Crawford et al 1995) and one (OarMP4) was derived from a hamster somatic cell hybrid containing part of the sheep X chromosome (Burkin et al 1993). …”

Section: Resultsmentioning

confidence: 99%

“…OarCP131 was isolated from genomic sheep DNA using the method described in Hanrahan et al (1994). OarMP4 was derived from DNA from a hamster somatic cell hybrid R612-3B10 (Burkin et al 1993), containing part of the sheep X chromosome (S.M. Galloway, unpubl.…”

Section: Genome Research ~ 673mentioning

confidence: 99%

“…TGLA primer sequences were designed from the sequence provided in the patent (Georges and Massey 1992) and may be different from the published bovine primer sequences for the same marker. Polymorphic information content (PIC, Botstein et al 1980) was calculated from the allele frequencies detected in 17 unrelated animals comprising the grandparents of the AgResearch International Mapping Flock (Crawford et al 1995) or the allele frequencies in 35 unrelated Romney and Coopworth female sheep (a).(Touchdown) touchdown PCR annealing procedure 60~ x3, 57~ x3, 54~ x3, 51~ • 48~ x20.marker isolation strategy used for the construction of the sheep linkage map (Crawford et al 1995) and one (OarMP4) was derived from a hamster somatic cell hybrid containing part of the sheep X chromosome (Burkin et al 1993). …”

mentioning

confidence: 99%

See 2 more Smart Citations

A linkage map of the ovine X chromosome.

Galloway

Hanrahan²,

Dodds³

et al. 1996

Genome Res.

View full text Add to dashboard Cite

A genetic linkage map of the ovine X chromosome containing type I and type II markers has been constructed. The map contains 7 known gene markers and 14 microsatellite markers with a recombination length of 141.9 cM. Segregation of polymorphic markers was observed in a three-generation pedigree containing 480 animals. The maximum number of informative meioses was 912. Additional information was obtained for some markers by following segregation in the AgResearch International Mapping Flock, consisting of nine three-generation full-sib pedigrees. A pseudoautosomal region containing two markers has been identified at one end of the linkage map. Comparisons with mouse and human X chromosomes confirms the observation of Ohno (1973} that the gene content of the mammalian X chromosome is retained. In particular, the conserved grouping of the genes PHKA1, ATP7A, and XIST observed in both the human and the mouse X chromosome appears to be conserved in the sheep X chromosome, and XIST has been mapped to near the center of the chromosome. This study provides the first reported genetic linkage map combining both type I and type 11 markers for any ruminant X chromosome.Until very recently, the X chromosomes of placental mammals were considered to be constrained by Ohno's rule (Ohno 1973), which postulated that genes on the X chromosome of one mammal would be on the X chromosomes of all other mammals. This rule was refined with the discovery that several genes on the short arm of the human X chromosome (Xp) are autosomal in marsupials and monotremes (McKay et al. 1992;Fitzgerald et al. 1993;Graves and Foster 1994). The apparent conservation of gene content of the X chromosomes was thought to be related to the requirement of X-inactivation in females for dosage compensation. The only known exception to this conservation in placental mammals has been a chloride channel gene (CLCN4), which has been shown recently to map to the X chromosome in humans and Mus spretus mice, but to chromosome 7 in C57BL/6 mice (Palmer et al. 1995;Rugarli et al. 1995). The X chromosomes of humans and mice are the most well-characterized and many known X-linked genes and other unique DNA sequences have been mapped in these species (Herman et al. 1994;Willard et al. 1994). Based on mapped gene orders, evolutionary relationships between mice and human X 3Corresponding author. E-MAIL galloways@agresearch.cri.nz; FAX 64-3-477-5413. chromosomes have been explained by five breakpoints and rearrangement of the resulting six blocks of genes (Amar et al. 1988). This concept has been refined recently to accommodate eight conserved segments with seven breakpoints (Blair et al. 1994(Blair et al. , 1995. It is of interest to determine whether the conserved blocks of genes identified in humans and mice remain conserved in other mammalian species, and whether the conservation of these groups has been maintained throughout evolution.The X chromosomes of mammalian species other than human and mouse are less wellcharacterized. In cattle, the genes DMD (Duchenne muscul...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Genome Research ~ 673mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

A linkage map of the ovine X chromosome.

Galloway

Hanrahan²,

Dodds³

et al. 1996

Genome Res.

View full text Add to dashboard Cite

show abstract

“…The translocations discovered by Bruere and coworkers are "marker" chromosomes that have been extremely useful in sheep gene-mapping studies (Ansari et al, 1993a;Burkin et al, 1993). When animals homozygous for ti, t2, t4 and t5 become available, this usefulness will be extended, as approximately 50% of the haploid genome will be represented in fused chromosomes (Matejka and Cribiu, 1987).…”

Section: Resultsmentioning

confidence: 99%

New Robertsonian translocation chromosomes in domestic sheep (<i>Ovis arie</i><i>s</i>)

Pearce

Ansari

Maher

et al. 1994

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lupton et al (1990) Second, through gene-mapping studies, which are the sub ject o f this report, we have extended the cytological evidence by presenting comparative mapping data demonstrating that there is conserved synteny between sheep chromosome 9 and cattle chromosome 14. The first evidence for genetic homology between sheep chromosome 9 and cattle chromosome 14 was the demonstration by Burkin et al (1993) that two loci, avian myelocytomatosis viral (v-myc) oncogene homolog (MYC) and Moloney murine sarcoma viral (x-mos) oncogene homolog (MOS), which map to cattle chromosome 14 (Threadgill et al. 1990), were on sheep chromosome 9.…”

mentioning

confidence: 99%

Assignment of five loci from human chromosome 8q onto sheep chromosome 9

Broad

Burkin

Cambridge

et al. 1995

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

Using a chromosomally characterized minipanel of sheep × hamster cell hybrids, five new loci, including carbonic anhydrase II (CA2), calbindin 1 (28 kDa) (CALB1), corticotropin releasing hormone (CRH), cytochrome P450 11B subfamily XIB (steroid-11-β-hydroxylase), polypeptide 1 (CYP11B1), and interleukin 7 (IL7), have been assigned to sheep chromosome 9. A homolog of mosome 9 as the sheep equivalent of cattle chromosome 14 and indicates that CALB1, CYP11B1, and IL7, which have not been mapped on the cattle genome, are likely to be present on cattle chromosome 14. It also shows by comparative genome analysis that a large segment of human chromosome 8q is highly conserved in sheep chromosome 9 and cattle chromosome 14. Based on these data, we propose that sheep chromosome 9 be recognised as the equivalent of cattle chromosome 14.

show abstract

Mapping the Sheep Genome: Production of Characterized Sheep X Hamster Cell Hybrids

Cited by 28 publications

References 0 publications

A linkage map of the ovine X chromosome.

A linkage map of the ovine X chromosome.

New Robertsonian translocation chromosomes in domestic sheep (<i>Ovis arie</i><i>s</i>)

Assignment of five loci from human chromosome 8q onto sheep chromosome 9

Contact Info

Product

Resources

About