Assignment of five loci from human chromosome 8q onto sheep chromosome 9

Abstract

Using a chromosomally characterized minipanel of sheep × hamster cell hybrids, five new loci, including carbonic anhydrase II (CA2), calbindin 1 (28 kDa) (CALB1), corticotropin releasing hormone (CRH), cytochrome P450 11B subfamily XIB (steroid-11-β-hydroxylase), polypeptide 1 (CYP11B1), and interleukin 7 (IL7), have been assigned to sheep chromosome 9. A homolog of mosome 9 as the sheep equivalent of cattle chromosome 14 and indicates that CALB1, CYP11B1, and IL7, which have not been mapped on the cattle genome, are likely to be present on cattle chromosome 14. It also shows by comparative genome analysis that a large segment of human chromosome 8q is highly conserved in sheep chromosome 9 and cattle chromosome 14. Based on these data, we propose that sheep chromosome 9 be recognised as the equivalent of cattle chromosome 14.

Regional Assignment of Elastin (ELN) to Sheep Chromosome 24q16-qter

“…Isotopic in situ hybridization was performed essentially as described by BROAD et al (1995) on early metaphase chromosomes from a ram with three Robertsonian translocations, karyotype 48XY, t,t,t,t,t,t,, where t, = rob 6;24, t, = rob 9;10, and t, = rob 7;25. The chromosomes were Q-banded before hybridization and identified according to the Reading standard karyotype (FORD et al 1980;ANSARI et al ,1994.…”

Genomic organization and assignment of the interleukin 7 gene (IL7) to porcine chromosome 4q11→q13 by FISH and by analysis of radiation hybrid panels

“…The IL7 gene has been also mapped to bovine chromosome 14 (BTA 14) (Barendse et al, 1997;Band et al, 2000) and ovine chromosome 9 (OVR 9) (Broad et al, 1995). It has been already shown that BTA 14 and OVR 9 have chromosome-wide synteny with the long arm of human chromosome 8 (Band et al, 2000;Burkin et al, 1997).…”

Physical mapping confirms that sheep chromosome 10 has extensive conserved synteny with cattle chromosome 12 and human chromosome 13

“…Isotopic in situ hybridization was performed as previously described (Broad et al 1995b) on early metaphase chromosomes from a ewe with three Robertsonian translocations (karyotype 49XX, t,t,t2t,t,, where t, =rob 6;24, t, = rob 9;10, and t,=rob 7;25). The chromosomes were Qbanded before hybridization and identified as in Ansari et al (1993a) according to the Reading standard karyotype (Ford et al 1980).…”