Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

Gussakovsky, Eugene; Yang, Yanmin; Rendell, Jennifer M; Jilkina, Olga; Kupriyanov, V.V.

doi:10.1016/j.ab.2010.07.010

Cited by 11 publications

(6 citation statements)

References 44 publications

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“…On the other hand, LCTF is a solid-state instrument that utilizes electronically controlled liquid crystal cells to transmit light with a specific wavelength and the elimination of all other wavelengths. The LCTF approach also has been used in coupling with the microscope, 65,[89][90][91][92] fundus microscope, 114,[117][118][119] and camera lens [56][57][58]60,62,64,[68][69][70]77 to create spectral image cubes for biological imaging in vivo and in vitro.…”

Section: Staring Biomedical Spectral Imaging Systemsmentioning

confidence: 99%

Review of spectral imaging technology in biomedical engineering: achievements and challenges

et al. 2013

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Spectral imaging is a technology that integrates conventional imaging and spectroscopy to get both spatial and spectral information from an object. Although this technology was originally developed for remote sensing, it has been extended to the biomedical engineering field as a powerful analytical tool for biological and biomedical research. This review introduces the basics of spectral imaging, imaging methods, current equipment, and recent advances in biomedical applications. The performance and analytical capabilities of spectral imaging systems for biological and biomedical imaging are discussed. In particular, the current achievements and limitations of this technology in biomedical engineering are presented. The benefits and development trends of biomedical spectral imaging are highlighted to provide the reader with an insight into the current technological advances and its potential for biomedical research.

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Section: Staring Biomedical Spectral Imaging Systemsmentioning

confidence: 99%

Review of spectral imaging technology in biomedical engineering: achievements and challenges

et al. 2013

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“…The theoretical background has been developed in detail elsewhere [20,21,24]. Below it is described in brief.…”

Section: Theoreticalmentioning

confidence: 99%

“…Recently, the method has been reported to determine maps of the absolute concentration of Mb, its oxygenation and optical pathlength in buffer-perfused arrested pig heart with a 0.4 mm spatial resolution, rapidly, noninvasively and without mechanical contact with the object [24]. The mapping is the result of the NIR spectroscopic imaging and employing a first-derivative approach of the pseudo-optical density spectrum.…”

Section: Introductionmentioning

confidence: 99%

NIR spectroscopic imaging to map hemoglobin + myoglobin oxygenation, their concentration and optical pathlength across a beating pig heart during surgery

Gussakovsky

Yang

Rendell

et al. 2011

Journal of Biophotonics

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The purpose of this paper is to demonstrate that near‐infrared (NIR) spectroscopic imaging can provide spatial distribution (maps) of the absolute concentration of hemoglobin + myoglobin, oxygen saturation parameter and optical pathlength, reporting on the biochemico‐physiological status of a beating heart in vivo. The method is based on processing the NIR spectroscopic images employing a first‐derivative approach. Blood‐pressure‐controlled gating compensated the effect of heart motion on the imaging. All the maps are available simultaneously and noninvasively at a spatial resolution in the submillimeter range and can be obtained in a couple of minutes. The equipment has no mechanical contact with the tissue, thereby leaving the heart unaffected during the measurement. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…22 The spectroscopic images were detected with a NIR system consisting of a Hamamatsu C7042 back-thinned FFTcharge coupled device (CCD) camera (Japan), Nikon AF-60 microlens and a Cambridge Research and Instrumentation, Inc. (Woburn, Massachusetts) VariSpec SNIR/NNIR liquid crystal tunable filter (LCTF). The system was assembled and operating software was developed at the NRC Institute for Biodiagnostics.…”

Section: Diffuse Reflectance and Fluorescence Imaging Of Rhod800 In Tmentioning

confidence: 99%

Rhodamine 800 as a near-infrared fluorescent deposition flow tracer in rodent hearts

et al. 2011

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Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

Cited by 11 publications

References 44 publications

Review of spectral imaging technology in biomedical engineering: achievements and challenges

Review of spectral imaging technology in biomedical engineering: achievements and challenges

NIR spectroscopic imaging to map hemoglobin + myoglobin oxygenation, their concentration and optical pathlength across a beating pig heart during surgery

Rhodamine 800 as a near-infrared fluorescent deposition flow tracer in rodent hearts

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