Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database

Brink, Daniel P.; Ravi, Krithika; Lidén, Gunnar; Gorwa-Grauslund, Marie-Françoise

doi:10.1007/s00253-019-09692-4

Cited by 93 publications

(74 citation statements)

References 291 publications

(217 reference statements)

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“…In the present work, we were able to isolate several bacterial strains from organosolv lignin cultures, belonging mainly to the gamma-and secondarily to the beta-proteobacteria. Bacteria belonging to the Proteobacteria phylum are commonly isolated from lignin and lignin model compounds enrichment studies (Brink et al 2019). Among them, many strains fall into Pseudomonas genus, as reported by several studies (Bandounas et al 2011;Ravi et al 2017;Hirose et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Microbial bioprospecting for lignocellulose degradation at a unique Greek environment

Georgiadou

Avramidis

Iοannou

et al. 2020

Preprint

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Bacterial systems have gained wide attention for depolymerization of lignocellulosic biomass, due to their high functional diversity and adaptability. To achieve the full microbial exploitation of lignocellulosic residues and the cost-effective production of bioproducts within a biorefinery, multiple metabolic pathways and enzymes of various specificities are required. In this work, highly diverse aerobic, mesophilic bacteria enriched from Keri Lake, a pristine marsh of increased biomass degradation and natural underground oil leaks, were explored for their metabolic versatility and enzymatic potential towards lignocellulosic substrates. A wide diversity of Pseudomonas species were obtained from enrichment cultures where organosolv lignin served as the sole carbon and energy source and were able to assimilate a range of lignin-associated aromatic compounds. Highly complex bacterial consortia were also enriched in cultures with xylan or carboxymethyl cellulose as sole carbon sources, belonging to Actinobacteria, Proteobacteria, Bacilli, Sphingobacteriia, and Flavobacteria. Numerous individual isolates could target diverse structural lignocellulose polysaccharides by expressing hydrolytic activities on crystalline or amorphous cellulose and xylan. Specific isolates showed increased potential for growth in lignin hydrolysates prepared from alkali pretreated agricultural wastes. The results suggest that Keri isolates represent a pool of effective lignocellulose degraders with significant potential for industrial applications in a lignocellulose biorefinery.

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Section: Discussionmentioning

confidence: 99%

Microbial bioprospecting for lignocellulose degradation at a unique Greek environment

Georgiadou

Avramidis

Iοannou

et al. 2020

Preprint

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“…In (E), plant roots gain access to inaccessible forms of N and P by stimulating the growth and induce priming activity of phenolic acid-degrading bacteria by exuding phenolic / aromatic acids. Table S1. A curated list of all isolates with characterized PHB degrading activity sourced from the eLignin database (http://www.elignindatabase.com/) [122]. Table S2.…”

Section: Competing Interestsmentioning

confidence: 99%

Phenolic acid-degradingParaburkholderiaprime decomposition in forest soil

Wilhelm

DeRito

Shapleigh

et al. 2020

Preprint

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Plant-derived phenolic acids are metabolized by soil microorganisms whose activity may enhance the decomposition of soil organic carbon (SOC). We characterized whether phenolic acid-degrading bacteria would enhance SOC mineralization in forest soils when primed with 13C-labeled p-hydroxybenzoic acid (PHB). We further investigated whether PHB-induced priming could explain differences in SOC content among mono-specific tree plantations in a 70-year-old common garden experiment. The activity of Paraburkholderia and Caballeronia dominated PHB degradation in all soils regardless of tree species or soil type. We isolated the principal PHB-degrading phylotype (Paraburkholderia madseniana RP11T), which encoded numerous oxidative enzymes, including secretion signal-bearing laccase, aryl-alcohol oxidase and DyP-type peroxidase, and confirmed its ability to degrade phenolics. The addition of PHB to soil led to significant enrichment (23-fold) of the RP11T phylotype (RP11ASV), as well as enrichment of other phylotypes of Paraburkholderia and Caballeronia. Metabolism of PHB primed significant loss of SOC (3 to 13 µmols C g-1 dry wt soil over 7 days). In contrast, glucose addition reduced SOC mineralization (−3 to -8 µmols C g-1 dry wt soil over 7 days). RP11ASV abundance and the expression of PHB monooxygenase (pobA) correlated with PHB respiration and were inversely proportional to SOC content in the field. We propose that plant-derived phenolics stimulate the activity of phenolic acid-degrading bacteria thereby causing soil priming and SOC loss. We show that Burkholderiaceae dominate soil priming in diverse forest soils and this observation counters the prevailing view that priming phenomena are a generalized non-specific response of community metabolism.

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“…Hence, the LccA enzyme could serve as valuable biotechnological tool for the removal of phenolic contaminants in phenolladen saline wastewater derived from various industrial settings (Moussavi et al 2010). Despite the potential of LccA in biofuel industry, its direct in vivo lignolytic activity on lignin has still to be experimentally verified (Brink et al 2019).…”

Section: Biotechnological Potential Of H Volcaniimentioning

confidence: 99%

Haloferax volcanii for biotechnology applications: challenges, current state and perspectives

Haque

Paradisi

Allers

2019

Appl Microbiol Biotechnol

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Haloferax volcanii is an obligate halophilic archaeon with its origin in the Dead Sea. Simple laboratory culture conditions and a wide range of genetic tools have made it a model organism for studying haloarchaeal cell biology. Halophilic enzymes of potential interest to biotechnology have opened up the application of this organism in biocatalysis, bioremediation, nanobiotechnology, bioplastics and the biofuel industry. Functionally active halophilic proteins can be easily expressed in a halophilic environment, and an extensive genetic toolkit with options for regulated protein overexpression has allowed the purification of biotechnologically important enzymes from different halophiles in H. volcanii. However, corrosion mediated damage caused to stainless-steel bioreactors by high salt concentrations and a tendency to form biofilms when cultured in high volume are some of the challenges of applying H. volcanii in biotechnology. The ability to employ expressed active proteins in immobilized cells within a porous biocompatible matrix offers new avenues for exploiting H. volcanii in biotechnology. This review critically evaluates the various application potentials, challenges and toolkits available for using this extreme halophilic organism in biotechnology.

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Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database

Cited by 93 publications

References 291 publications

Microbial bioprospecting for lignocellulose degradation at a unique Greek environment

Microbial bioprospecting for lignocellulose degradation at a unique Greek environment

Phenolic acid-degradingParaburkholderiaprime decomposition in forest soil

Haloferax volcanii for biotechnology applications: challenges, current state and perspectives

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