Haloferax volcanii for biotechnology applications: challenges, current state and perspectives

Haque, Risat Ul; Paradisi, Francesca; Allers, Thorsten

doi:10.1007/s00253-019-10314-2

Cited by 40 publications

(24 citation statements)

References 126 publications

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“…Halophilic organisms constitute a promising and relatively under-explored target for biotechnological applications due to the particular conditions their physiology requires (Singh and Singh 2017;Jia et al 2017;Haque et al 2019;Haque et al 2020). The halophilic archaeon Haloferax volcanii has become an important archaeal model system, as it is an organism that can be easily cultivated in the laboratory and for which many biochemical, molecular and genetic tools have been developed (Pohlschr€ oder and Schulze 2019).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and kinetic study of silver and gold nanoparticles produced by the archaeon Haloferax volcanii

Costa

Álvarez-Cerimedo

Urquiza

et al. 2020

J. Appl. Microbiol.

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Aims: To evaluate the ability of the haloarchaeon Haloferax volcanii to produce Ag and Au nanoparticles (NPs) and to characterize the obtained material in order to find relevant properties for future potential applications. Methods and Results: Nanoparticles were produced by incubating H. volcanii cells with the corresponding metal salt. In the presence of precursor salts, cultures evidenced a colour change associated to the formation of metallic nanostructures with plasmonic bands located in the visible range of the spectrum. X-ray fluorescence analysis confirmed the presence of Ag and Au in the NPs which were spherical, with average sizes of 25 nmol l À1 (Ag) and 10 nmol l À1 (Au), as determined by electronic microscopy. Fourier transformed infrared spectroscopy indicated that both types of NPs showed a stable protein capping. Ag NPs evidenced antibacterial activity and Au NPs improved the specificity of polymerase chain reaction reactions. Au and Ag NPs were able to reduce 4-nitrophenol when incubated with NaBH 4. Conclusions: Haloferax volcanii is able to synthesize metallic NPs with interesting properties for technological applications. Significance and Impact of the Study: Our data demonstrate the ability of H. volcanii to synthesize metal NPs and constitutes a solid starting point to deepen the study and explore novel applications.

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Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and kinetic study of silver and gold nanoparticles produced by the archaeon Haloferax volcanii

Costa

Álvarez-Cerimedo

Urquiza

et al. 2020

J. Appl. Microbiol.

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“…Diffraction limited snapshots of DNA-Hoechst 33342 were taken after SMLM imaging. b) The H119 strain synthesizes lycopene and its derivatives (Haque et al, 2020) which can be easily seen by bright red colonies on agar-plates. These cause autofluorescence background that largely hinders single-molecule readout.…”

Section: Discussionmentioning

confidence: 99%

Establishing live-cell single-molecule localization microscopy imaging and single-particle tracking in the archaeonHaloferax volcanii

Turkowyd

Schreiber

Wörtz

et al. 2020

Preprint

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In recent years, fluorescence microscopy techniques for the localization and tracking of single molecules in living cells have become well-established and indispensable tools for the investigation of cellular biology and in vivo biochemistry of many bacterial and eukaryotic organisms. Nevertheless, these techniques are still not established for imaging archaea. Their establishment as a standard tool for the study of archaea will be a decisive milestone for the exploration of this branch of life and its unique biology.Here we have developed a reliable protocol for the study of the archaeon Haloferax volcanii. We have generated an autofluorescence-free H. volcanii strain, evaluated several fluorescent proteins for their suitability to serve as single-molecule fluorescence markers and codon-optimized them to work under optimal H. volcanii cultivation conditions. We found that two of them, Dendra2Hfx and PAmCherry1Hfx, provide state-of-the-art single-molecule imaging. Our strategy is quantitative and allows dual-color imaging of two targets in the same field of view as well as DNA co-staining. We present the first single-molecule localization microscopy (SMLM) images of the subcellular organization and dynamics of two crucial intracellular proteins in living H. volcanii cells, FtsZ1, which shows complex structures in the cell division ring, and RNA polymerase, which localizes around the periphery of the cellular DNA. This work should provide incentive to develop SMLM strategies for other archaeal organisms in the near future.

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“…For FOVs containing both strains, first bright light images as well as images of green preconverted Dendra2Hfx fluorescence were taken. Dendra2Hfx was read-out using primed photoconversion The H119 strain synthesizes lycopene and its derivatives (Haque et al, 2020), which can be easily seen by bright red colonies on agar-plates. These cause autofluorescence background that largely hinders single-molecule read-out.…”

Section: Dual-color Imaging Of Cells Producing Ftsz1-dendra2hfx and Fmentioning

confidence: 99%

Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii

et al. 2020

View full text Add to dashboard Cite

In recent years, fluorescence microscopy techniques for the localization and tracking of single molecules in living cells have become well-established and are indispensable tools for the investigation of cellular biology and in vivo biochemistry of many bacterial and eukaryotic organisms. Nevertheless, these techniques are still not established for imaging archaea. Their establishment as a standard tool for the study of archaea will be a decisive milestone for the exploration of this branch of life and its unique biology. Here, we have developed a reliable protocol for the study of the archaeon Haloferax volcanii. We have generated an autofluorescence-free H. volcanii strain, evaluated several fluorescent proteins for their suitability to serve as single-molecule fluorescence markers and codon-optimized them to work under optimal H. volcanii cultivation conditions. We found that two of them, Dendra2Hfx and PAmCherry1Hfx, provide state-of-the-art single-molecule imaging. Our strategy is quantitative and allows dual-color imaging of two targets in the same field of view (FOV) as well as DNA co-staining. We present the first single-molecule localization microscopy (SMLM) images of the subcellular organization and dynamics of two crucial intracellular proteins in living H. volcanii cells, FtsZ1, which shows complex structures in the cell division ring, and RNA polymerase, which localizes around the periphery of the cellular DNA.This work should provide incentive to develop SMLM strategies for other archaeal organisms in the near future.

show abstract

Haloferax volcanii for biotechnology applications: challenges, current state and perspectives

Cited by 40 publications

References 126 publications

Synthesis, characterization and kinetic study of silver and gold nanoparticles produced by the archaeon Haloferax volcanii

Synthesis, characterization and kinetic study of silver and gold nanoparticles produced by the archaeon Haloferax volcanii

Establishing live-cell single-molecule localization microscopy imaging and single-particle tracking in the archaeonHaloferax volcanii

Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii

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