Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses

Wang, Yong; Xie, Jingzhong; Zhang, Huaizhi; Guo, Bingmin; Ning, Shunzong; Chen, Yongxing; Lü, Ping; Wu, Qiuhong; Li, Miaomiao; Zhang, Deyun; Guo, Guanghao; Zhang, Yan; Liu, Dengcai; Zou, Shaokui; Tang, Jianwei; Zhao, Hong; Wang, Xicheng; Li, Jun; Yang, Wuyun; Cao, Tingjie; Guo, Yin; Li, Yong

doi:10.1007/s00122-017-2950-0

Cited by 72 publications

(33 citation statements)

References 38 publications

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“…Contrast with the traditional methods, BSR-Seq could provide adequate genes information, such as SNPs, gene expression data, which greatly accelerate the process of gene mapping. And now the BSR-Seq mapping strategy was widely used to map the key genes in Brassica, maize, wheat and other species [23][24][25][26]. This study BSR-Seq approach was used to locate the yellow seed color gene to A09 chromosome and the information of gene expression on A09 chromosome was also provided, which is helpful for screening candidate genes, therefore BSR-Seq is a powerful method for gene mapping and cloning.…”

Section: Discussionmentioning

confidence: 99%

Mapping and identification of yellow seed coat color genes in Brassica juncea L.

Huang

Wang

Lü³

et al. 2020

Preprint

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BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

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Section: Discussionmentioning

confidence: 99%

Mapping and identification of yellow seed coat color genes in Brassica juncea L.

Huang

Wang

Lü³

et al. 2020

Preprint

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“…Previously deployed resistance genes lose effectiveness and yield losses caused by the emergence of new Pst races (Schwessinger 2017). A new Pst race (V26/CYR34) was virulent to resistance gene Yr26 and spread in several major wheat growing regions in the wheat breeding programs (Liu et al 2010, Wang et al 2017. Recently, some study reported that about 76% of current wheat cultivars and breeding lines are susceptible to CYR34 (Han et al 2015, Bai et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveal the mechanism of susceptible wheat seedlings response to Puccinia striiformis f. sp.

Liu¹,

Chen²,

Zhou³

et al. 2020

Preprint

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Background Wheat (Triticum aestivum L.) is most widely cultivated and a major staple food crops in the world. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), which significantly reduce yield and quality of wheat. Although some resistant genes have been successfully used in wheat breeding, large of the regulating networks and the underlying molecular mechanisms of Pst response remains unknown. Therefore, to identify differentially expressed genes (DEGs) and regulate network involved in Pst resistance, we sequenced 15 cDNA libraries constructed from wheat seedlings with CYR34 infection.Results In this study, a highly susceptible cv. Chuanyu12 (CY12) were used to study the transcriptome profiles after inoculated with Pst physiological race CYR34. A total of 13892, 10195, 12268 and 14044 DEGs were investigated at 24h, 48h, 72h and 7days Pst infection, respectively. Certain key genes and pathways responsible for Pst-CYR34 in CY12 were identified. The results revealed that Pst-CYR34 inhibited the DEGs related to energy metabolism, biosynthesis, carbon fixation, phenylalanine metabolism, and plant hormone signaling pathway after Pst inoculation at 24h, 48h, 72h and 7d. These down-regulated DEGs including light-harvesting chlorophyll protein complex in photosystem I and photosystem II; cytochrome b6/f/ complex, F-type ATPase and photosynthetic electron transport; ethylene, jasmonic acid (JA) and salicylic acid (SA); lignin and flavonoids biosynthesis in CY12. Quantitative Real-time PCR analysis verified the expression patterns of these DEGs.Conclusions Our results give insights into the foundation for further exploring the molecular mechanism regulating networks of Pst response and pave the way for durable resistant breeding in bread wheat.

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“…Yr26 was widely used in the wheat breeding programs [10][11][12]. Recently, some study reported that most of current wheat cultivars and breeding lines (76%) are susceptible to CYR34 [13].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis reveals the molecular mechanisms of wheat seedling resistance to Puccinia striiformis f. sp. tritici

Liu¹,

Lu²,

Du³

et al. 2020

Preprint

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Background: Stripe rust or yellow rust (Yr), caused by Puccinia striiformis f. sp. Tritici (Pst), is one of the most globally devastating fungal disease that significantly reduces yield and quality in wheat (Triticum aestivum). Although some Yr genes have been successfully used in wheat breeding and a little number of them have been cloned, large of the regulating networks and the molecular mechanisms of Pst resistance remains unknown. In this study, a pair of Yr-gene pyramiding line L58 and its background parent cv. Chuanyu12 (CY12) were used to study the transcriptome profiles after inoculated with Pst physiological race CYR34. Results: The results revealed that the different expression genes (DEGs) were significantly enriched in phenylpropanoid biosynthesis, phenylalanine metabolism, plant-pathogen interaction and MAPK signaling pathways after Pst-CYR34 inoculation. Compared with CY12, L58 showed greater up-regulated DEGs in those pathways by Pst infection at 24hpi. However, these DEGs became lower expression in L58 and opposite expression in CY12 at 7dpi. Besides, the activities of enzymes (PAL, POD) and products of phenylpropanoid pathway (lignin content) were significantly increased in both CY12 and L58, and the increase was greater and faster in the resistant line L58. Some candidate genes and transcription factors (TFs) associated with Pst resistance were identified, including LRR receptor-like serine/threonine protein kinase, disease resistance protein, MYB, NAC and WRKY transcription factors involved in the fine-tuning of Pst infection responses. Conclusions: Our results give insights into the regulating networks of Pst resistance and pave the way for durable resistant breeding in bread wheat.

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Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses

Cited by 72 publications

References 38 publications

Mapping and identification of yellow seed coat color genes in Brassica juncea L.

Mapping and identification of yellow seed coat color genes in Brassica juncea L.

Transcriptome analysis reveal the mechanism of susceptible wheat seedlings response to Puccinia striiformis f. sp.

Transcriptomic analysis reveals the molecular mechanisms of wheat seedling resistance to Puccinia striiformis f. sp. tritici

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