Mapping Rare Erythrocyte Phenotypes in Morocco: A Tool to Overcome Transfusion Challenges

Benahadi, A.; Boulahdid, S.; Adouani, B.; Laouina, A.; Mokhtari, Abdelrhani; Soulaymani, Abdelmajid; Hajjout, K.; Benajiba, M.; Alami, Ramzi S.

doi:10.1155/2014/707152

Cited by 7 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study from Morocco, KEL1 (K) is 0.19% whereas KEL2 is 99.80% (Benahadi et al, 2014). Similar study conducted in Southern Brazil documented the frequencies as KEL*02/KEL*02: 94 (75%), KEL*01/KEL*02: 5.0%, while KEL*01/KEL*01 was 0.25% (Guelsin et al, 2011;Flôres et al, 2014).…”

Section: Discussionmentioning

confidence: 85%

Molecular organisation of Kell (KEL) red blood cell variants among voluntary blood donors at the National Blood Grouping Testing Laboratory, Kenya

Rachel¹,

Kennedy²,

Suleiman³

et al. 2022

J. Bioinform. Seq. Anal.

View full text Add to dashboard Cite

A variant is an alternative nucleotide located at a specific region of a gene. 48 genes encode for human red cell blood group systems. Variants within these genes encode for alleles, which can be highly polymorphic. The blood group gene loci jointly display all types of inherited variants to include single nucleotide variants (SNVs), insertions/deletions (indels), and structural variants. In Africa, there is limited information on the red cell variants. The aim of the study was to determine the incidence of Kell red blood cell variants among the Kenyans. Blood donor's samples that were used for routine grouping process were identified for this study. Next generation sequencing method was employed to predict the Kell red cell variants in blood donor samples. Descriptive statistics was applied and the result was presented in form of tables. The study reveals that Kell system has six variants with two major phenotypes and genotpes. The most common phenotype genotyped was KEL2 (KEL*02/KEL*02) 79% (85/108) followed by KEL2 KEL6 KEL7 (KEL*02.06/KEL*02) 16.7% (18/108). The rest were found to be of low frequency and all were associated with KEL2. The study recommends an extended study with a large sample size and introduction of extended phenotyping to aid Kell antigens identification in the donor population.

show abstract

Section: Discussionmentioning

confidence: 85%

Molecular organisation of Kell (KEL) red blood cell variants among voluntary blood donors at the National Blood Grouping Testing Laboratory, Kenya

Rachel¹,

Kennedy²,

Suleiman³

et al. 2022

J. Bioinform. Seq. Anal.

View full text Add to dashboard Cite

show abstract

“…In South Africa, black population Fy(a-b-) 39% while Fya+ Fyb+ was reported to be 2% (Govender et al, 2021). In Saudi Arabia, type Fy(a-b-) was identified in 11.11% (Benahadi et al, 2014). In the Brazilian Japanese population, FY study has shown that FY * 01/FY*01(Fya+) occurrence was 59.33%, FY*01/FY*02 (Fya+ Fyb+) was 38.27% and FY*02/FY*02 (Fyb+) was 2.39% (Flôres et al, 2014;Höher et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation of Duffy (FY) red blood cell variants among voluntary blood donors at the National Blood Grouping Testing Laboratory, Kenya

Rachel¹,

Kennedy²,

Suleiman³

et al. 2022

J. Bioinform. Seq. Anal.

View full text Add to dashboard Cite

A variant is an alternative nucleotide located at a specific region of a gene. 48 genes encode for human red cell blood group systems. Variants within these genes encode for alleles, which can be highly polymorphic. The blood group gene loci jointly display all types of inherited variants to include single nucleotide variants insertions/deletions and structural variants. In Africa, there is limited information on the red cell variants. The aim of the study was to establish the frequency Duffy red blood cell variants among the donors in Kenya. The study employed next sequencing method, descriptive statistics and results presented in form of a table. The findings show that Duffy system has three variants to include; homozygous for FY*Null GATA regulatory box variant FY*02N.01/FY*02N.01 or Fy(a-b-) found in 93.52% (101/108), while FY*01/FY*02 and FY c.-67T>C predicted Fy(a+b-) or Fy(a-b+) at 3.70% (4/108) while FY*02/FY*02N.01 predicted Fy(a-b+) at 2.78% (3/108). This study recommends an extended research involving large sample size and introduction of extended phenotyping in the identification of FY antigens population.

show abstract

“…The frequency of RhD negative varies between populations: 15% in Caucasians, 3-7% in Africans, 7-8% among Black Americans, and <1% in Asians [26][27][28]. Comparing different rhesus phenotypic frequencies, one can observe similarity between populations of Morocco, (DCcee: 38.85% dccee: 8.77 %), Maghreb (DCcee: 37.55% dccee: 6.12%) and Arab Gulf (DCcee: 30.9% dccee: 7.3%) [29][30][31]. These results confirm previous studies concerning ABO system.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of weak D phenotype among D negative C/E+ blood donors in Morocco

Wafi

Housse

Nourichafi

et al. 2016

Int J Blood Transfus Immunohematol

View full text Add to dashboard Cite

Aims: Determination of D variants is important for selection of blood products and to prevent anti-D-related hemolytic disease of the fetus and newborn. the prevalence of weak D phenotypes varies significantly among different ethnic populations. In Morocco, D variants have not been fully characterized. the purpose of this study was to determine prevalence of weak D among D negative and c/E+ blood donors and distribution of rhesus blood group antigens using serologic methods. Methods: A total of 15,865 blood samples from Moroccan blood donors were tested for D, c, c, E, e antigens by routine serologic methods. Among blood donors serologically D negative c+ and/or E+, 63 samples were tested for weak D by indirect antiglobuline test, enzymatic treated cells test and adsorption elution technique. results: Among 63 samples tested, 10 were positive by serologic methods

show abstract

Mapping Rare Erythrocyte Phenotypes in Morocco: A Tool to Overcome Transfusion Challenges

Cited by 7 publications

References 11 publications

Molecular organisation of Kell (KEL) red blood cell variants among voluntary blood donors at the National Blood Grouping Testing Laboratory, Kenya

Molecular organisation of Kell (KEL) red blood cell variants among voluntary blood donors at the National Blood Grouping Testing Laboratory, Kenya

Molecular characterisation of Duffy (FY) red blood cell variants among voluntary blood donors at the National Blood Grouping Testing Laboratory, Kenya

Prevalence of weak D phenotype among D negative C/E+ blood donors in Morocco

Contact Info

Product

Resources

About