Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers

Abstract

Growing resistant wheat (Triticum aestivum L) varieties is an important strategy for the control of leaf rust, caused by Puccinia triticina Eriks. This study sought to identify the chromosomal location and effects of leaf rust resistance loci in five Canadian spring wheat cultivars. The parents and doubled haploid lines of crosses Carberry/AC Cadillac, Carberry/Vesper, Vesper/Lillian, Vesper/Stettler and Stettler/Red Fife were assessed for leaf rust severity and infection response in field nurseries in Canada near Swift Current, SK from 2013 to 2015, Morden, MB from 2015 to 2017 and Brandon, MB in 2016, and in New Zealand near Lincoln in 2014. The populations were genotyped with the 90K Infinium iSelect assay and quantitative trait loci (QTL) analysis was performed. A high density consensus map generated based on 14 doubled haploid populations and integrating SNP and SSR markers was used to compare QTL identified in different populations. AC Cadillac contributed QTL on chromosomes 2A, 3B and 7B (2 loci), Carberry on 1A, 2B (2 loci), 2D, 4B (2 loci), 5A, 6A, 7A and 7D, Lillian on 4A and 7D, Stettler on 2D and 6B, Vesper on 1B, 1D, 2A, 6B and 7B (2 loci), and Red Fife on 7A and 7B. Lillian contributed to a novel locus QLr.spa-4A, and similarly Carberry at QLr.spa-5A. The discovery of novel leaf rust resistance QTL QLr.spa-4A and QLr.spa-5A, and several others in contemporary Canada Western Red Spring wheat varieties is a tremendous addition to our present knowledge of resistance gene deployment in breeding. Carberry demonstrated substantial stacking of genes which could be supplemented with the genes identified in other cultivars with the PLOS ONE PLOS ONE | https://doi.org/10.1371/journal.pone.0230855 April 8, 2020 1 / 22 OPEN ACCESS Citation: Bokore FE, Knox RE, Cuthbert RD, Pozniak CJ, McCallum BD, N'Diaye A, et al. (2020) Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers. PLoS ONE 15(4): e0230855. https://doi.org/ 10.

QTL Mapping for Seedling and Adult Plant Resistance to Leaf and Stem Rusts in Pamyati Azieva × Paragon Mapping Population of Bread Wheat

“…Even when parents demonstrated the same level of resistance, such as APR to SR, RILs still showed transgressive phenotypes in the direction of either resistance (RIBSP) or susceptibility (KRIAPI) (Table 3). This phenomenon is not rare; it was previously described for many other quantitatively inherited wheat traits; for example, in studies of grain quality traits [22], grain Zn and Fe concentrations [59], grain yield and plant height [60], and rust diseases [61,62].…”

Meta-QTLs and candidate genes for stripe rust resistance in wheat

“…A consensus genetic map involving SSR, DArT and SNP markers was constructed using LPmerge software 41 . For this purpose, five individual genetic linkage maps each from different studies were used as reference maps 42 – 46 . Markers flanking individual QTL regions were also included for construction of a consensus genetic map.…”

High density genetic mapping of stripe rust resistance in a ‘Strongfield’ / ‘Blackbird’ durum wheat population

“…Hou et al (2015) reported an all stage stripe rust HTAP resistance QTL on 5BL associated with wsnp_JD_rep_c50403_34392266 in a soft white winter wheat cultivar 'Druchamp' (PI 174622) introduced to the US Pacific Northwest for production in 1949 from France. This QTL may be controlled by the same factor as QYr.spa-5B mapping within 49 cM of QYr.spa-5B on the consensus map by Bokore et al (2020). Using the same consensus map, a peak marker BS00062762_51 for the QYr.spa-5B in 'Blackbird' was located 43.21 cM from wsnp_Ku_c4427_8029592, a marker associated with QYr.usw-5B in durum wheat line W9262-260D3 (Lin et al 2018).…”

“…Singh et al (2014) reported a stripe rust QTL on chromosome 5B associated with Xbarc59 in spring wheat cultivar 'AC Cadillac'. Xbarc59 is located 76.7 cM from the peak marker associated with QYr.spa-5B from 'Blackbird' on the hexaploid wheat SNP consensus map of Bokore et al (2020) suggesting the Singh et al (2014) QTL is different from QYr.spa-5B. Hou et al (2015) reported an all stage stripe rust HTAP resistance QTL on 5BL associated with wsnp_JD_rep_c50403_34392266 in a soft white winter wheat cultivar 'Druchamp' (PI 174622) introduced to the US Pacific Northwest for production in 1949 from France.…”