Mapping physiological and pathological functions of cortical vasculature through aggregation-induced emission nanoprobes assisted quantitative, in vivo NIR-II imaging

Meng, Jia; Feng, Zhe; Qian, Shuhao; Wang, Chuncheng; Li, Xinjian; Gao, Lixia; Ding, Zhihua; Qian, Jun; Liu, Zhiyi

doi:10.1016/j.bioadv.2022.212760

Cited by 12 publications

(9 citation statements)

References 54 publications

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“…Another possibility to overcome the mentioned challenge is to use labels with increased SWIR brightness, such as aggregation-induced emission (AIE) dots [38]. The high brightness of AIE dots was demonstrated to be vital for enabling biomedical applications such as mapping physiological and pathological functions of cortical vasculature and its response to stroke and thrombosis in animals [39]. Organic dyes like ICG, while having lower brightness per emitter, have the significant advantage of being clinically approved, significantly reducing the regulatory burden for potential medical applications in humans.…”

Section: Discussionmentioning

confidence: 99%

Shortwave-Infrared Line-Scan Confocal Microscope for Deep Tissue Imaging in Intact Organs

Lingg

Bischof

Rowlands

et al. 2022

Preprint

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The recent developments of fluorophores having photoemission beyond 1000nm provide the opportunity to develop novel fluorescent microscopes sensitive to those wavelengths. The imaging at wavelengths beyond the visible spectrum allows to achieve imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications. Here, a novel shortwave-infrared line-scan confocal microscope is presented that is capable of deep imaging of biological specimens, as demonstrated by visualization of labelled glomeruli in a fixed uncleared kidney at depths of 300 um to 400 um. This development improves the acquisition speed of deep tissue fluorescence imaging.

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Section: Discussionmentioning

confidence: 99%

Shortwave-Infrared Line-Scan Confocal Microscope for Deep Tissue Imaging in Intact Organs

Lingg

Bischof

Rowlands

et al. 2022

Preprint

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“…258 The morphological changes and organization remodeling of cortical blood vessels in marmosets during stroke have been fully and accurately analyzed. 259,260 Afterglow luminescence is an optical phenomenon where some substances keep emitting the light after stopping their optical excitation. 261 By means of this property, afterglow imaging exhibits many advantages when compared with traditional fluorescence imaging such as no background signal interference, ultrahigh sensitivity, no real-time excitation, etc.…”

Section: Near-infrared Imagingmentioning

confidence: 99%

“…Centimeter-deep and three-photon fluorescence NIR-II vascular imaging in nonhuman primates has also been realized. , Further, a series of quantitative approaches have been developed to assess the structural changes of deep vessels using biocompatible AIE dots . The morphological changes and organization remodeling of cortical blood vessels in marmosets during stroke have been fully and accurately analyzed. , …”

Section: Light Up Life: See the Unseenmentioning

confidence: 99%

Aggregation-Induced Emission (AIE), Life and Health

Wang

Alam

et al. 2023

ACS Nano

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Light has profoundly impacted modern medicine and healthcare, with numerous luminescent agents and imaging techniques currently being used to assess health and treat diseases. As an emerging concept in luminescence, aggregation-induced emission (AIE) has shown great potential in biological applications due to its advantages in terms of brightness, biocompatibility, photostability, and positive correlation with concentration. This review provides a comprehensive summary of AIE luminogens applied in imaging of biological structure and dynamic physiological processes, disease diagnosis and treatment, and detection and monitoring of specific analytes, followed by representative works. Discussions on critical issues and perspectives on future directions are also included. This review aims to stimulate the interest of researchers from different fields, including chemistry, biology, materials science, medicine, etc., thus promoting the development of AIE in the fields of life and health.

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“…Recently, we developed an automated, voxel-wise measure of thickness within fiber-like structures and applied it to the analysis of cerebrovascular diseases. 19 Based on the thickness information, in this study we propose a window optimizing (WO) method that is able to significantly enhance the determination accuracy of spatial orientation, for both 2D and 3D cases. As a fusion of thickness determination and a weighted orientation vector summation algorithm, the WO method adaptively optimizes calculating parameters at a pixel-wise basis according to fiber thickness information.…”

Section: Introductionmentioning

confidence: 99%

“…Specially designed AIE nanoparticles (NPs) were used to obtain large-depth 3D cerebrovascular image information. Recently, we developed an automated, voxel-wise measure of thickness within fiber-like structures and applied it to the analysis of cerebrovascular diseases 19 . Based on the thickness information, in this study we propose a window optimizing (WO) method that is able to significantly enhance the determination accuracy of spatial orientation, for both 2D and 3D cases.…”

Section: Introductionmentioning

confidence: 99%

Highly accurate, automated quantification of 2D/3D orientation for cerebrovasculature using window optimizing method

et al. 2022

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. Significance Deep-imaging of cerebral vessels and accurate organizational characterization are vital to understanding the relationship between tissue structure and function. Aim We aim at large-depth imaging of the mouse brain vessels based on aggregation-induced emission luminogens (AIEgens), and we create a new algorithm to characterize the spatial orientation adaptively with superior accuracy. Approach Assisted by AIEgens with near-infrared-II excitation, three-photon fluorescence (3PF) images of large-depth cerebral blood vessels are captured. A window optimizing (WO) method is developed for highly accurate, automated 2D/3D orientation determination. The application of this system is demonstrated by establishing the orientational architecture of mouse cerebrovasculature down to the millimeter-level depth. Results The WO method is proved to have significantly higher accuracy in both 2D and 3D cases than the method with a fixed window size. Depth- and diameter-dependent orientation information is acquired based on in vivo 3PF imaging and the WO analysis of cerebral vessel images with a penetration depth of in mice. Conclusions We built an imaging and analysis system for cerebrovasculature that is conducive to applications in neuroscience and clinical fields.

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Mapping physiological and pathological functions of cortical vasculature through aggregation-induced emission nanoprobes assisted quantitative, in vivo NIR-II imaging

Cited by 12 publications

References 54 publications

Shortwave-Infrared Line-Scan Confocal Microscope for Deep Tissue Imaging in Intact Organs

Shortwave-Infrared Line-Scan Confocal Microscope for Deep Tissue Imaging in Intact Organs

Aggregation-Induced Emission (AIE), Life and Health

Highly accurate, automated quantification of 2D/3D orientation for cerebrovasculature using window optimizing method

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