Highly accurate, automated quantification of 2D/3D orientation for cerebrovasculature using window optimizing method

Abstract

. Significance Deep-imaging of cerebral vessels and accurate organizational characterization are vital to understanding the relationship between tissue structure and function. Aim We aim at large-depth imaging of the mouse brain vessels based on aggregation-induced emission luminogens (AIEgens), and we create a new algorithm to characterize the spatial orientation adaptively with superior accuracy. Approach Assisted by AIEgens with near-infrared-II excitation, three-photon fluorescence (3PF) images of large-depth cerebral blood vessels are captured. A window optimizing (WO) method is developed for highly accurate, automated 2D/3D orientation determination. The application of this system is demonstrated by establishing the orientational architecture of mouse cerebrovasculature down to the millimeter-level depth. Results The WO method is proved to have significantly higher accuracy in both 2D and 3D cases than the method with a fixed window size. Depth- and diameter-dependent orientation information is acquired based on in vivo 3PF imaging and the WO analysis of cerebral vessel images with a penetration depth of in mice. Conclusions We built an imaging and analysis system for cerebrovasculature that is conducive to applications in neuroscience and clinical fields.

Aggregation-Induced Emission (AIE), Life and Health

“…The NIR-II fluorescence microscopic monitoring through-thinned-skulls of nonhuman primates brain vessels using microscopic monitoring and the NIR-IIb fluorescence GI imaging was successfully achieved (Figure A and B) . Centimeter-deep and three-photon fluorescence NIR-II vascular imaging in nonhuman primates has also been realized. , Further, a series of quantitative approaches have been developed to assess the structural changes of deep vessels using biocompatible AIE dots . The morphological changes and organization remodeling of cortical blood vessels in marmosets during stroke have been fully and accurately analyzed. , …”

“…256,257 Further, a series of quantitative approaches have been developed to assess the structural changes of deep vessels using biocompatible AIE dots. 258 The morphological changes and organization remodeling of cortical blood vessels in marmosets during stroke have been fully and accurately analyzed. 259,260 Afterglow luminescence is an optical phenomenon where some substances keep emitting the light after stopping their optical excitation.…”