Mapping of theLyb-4 gene to different chromosomes in DBA/2J and C3H/HeJ mice

Chromosomal locations of members of the xenotropic-related env gene family in the mouse genome have been determined. Endonuclease restriction site polymorphisms detected by molecular hybridization were used to.study the inheritance of mink cell-focus inducing and xenotropic env gene-related sequences in recombinant inbred strains of mice. Some of the endogenous env sequences, appear to be eloselylinked to genes determining leukemia virus induction and to genes involved in the immune response, such as the heavy and light chains of the immunoglobulin molecules or allotypic determinants on B and T lymphocytes. The use of probes that detect restriction fragment length polymorphisms in a small family of dispersed sequences promises to yield a large number of markers that can be used together with recombinant inbred strains for efficient mapping of the mouse genome.

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Chromosomal mapping of the mink cell focus-inducing and xenotropic env gene family in the mouse.

Blatt¹,

Mileham²,

Haas³

et al. 1983

“…Several other clusters of genes specifying leukocyte cell surface molecules have been identified by use of alloantisera or monoclonal antibodies: (i) H-2--Qa--Tla on chromosome 17 (21); (ii) Mls--Lgp 100 (Ly-m9)--LyM on chromosome 1 (22); (iii) Lyb-2--Lyb-4--Lyb-6 on chromosome 4 (23)(24)(25); (iv) Lyt-1--Ly-mlO on chromosome 19 (26); and (v) Lyt-2--Lyt-3 on chromosome 6 (27).…”

Section: Discussionmentioning

confidence: 99%

Gene for a major cell surface glycoprotein of mouse macrophages and other phagocytic cells is on chromosome 2.

Colombatti

Hughes

Taylor

et al. 1982

A gene controlling the expression of a polymorphic 92,000-dalton glycoprotein of mouse macrophages and granulocytes has been identified. This glycoprotein was previously shown to be the major iodinated, trypsin-sensitive component of the murine phagocyte cell surface. The gene has been provisionally designated Pgp-1 for phagocyte glycoprotein 1. Expression of the glycoprotein was measured by monoclonal antibody binding to a polymorphic antigenic determinant. Antibody binding to cells of positive strains of mice was proportional to cell number, whereas binding to cells of negative strains was insignificant. The concentration of the antigen in cells of heterozygous mice was approximately 50% of that in homozygous mice. Thirteen of 33 recombinant inbred strains of mice were positive, with binding values 100-fold over background, suggesting that a single gene controlled expression of the antigen. Segregation of the antigen correlated with markers on chromosome 2. The segregation of Pgp-i, with nonagouti coat color (a) and hemolytic complement (Hc) activity among progeny of(C3H/HeJ X DBA/2J)FI X DBA/

“…This histocompatibility antigen is defined by its ability to stimulate skin graft rejection and generation of CTL by B6 hosts. Because Mov-14 was generated by B6 pronucleus microinjection with cloned Mov-3 provirus plus 8 kilobases of ICR flanking sequences, the cross-reaction between Mov-14 and Mov-3 could have been due to the same histocompatibility antigen encoded by ICR strain flanking sequences (26). Accordingly, the typing of different inbred strains for alleles at single non-H-2 histocompatibility loci may be partially incorrect; the original typing by complementation testing was based on the assumption that genes encoding histocompatibility antigens defined by a specific congenic strain combination were alleles mapping to a single histocompatibility locus (27).…”

Section: Discussionmentioning

confidence: 99%

Endogenous retroviruses lead to the expression of a histocompatibility antigen detectable by skin graft rejection.

Colombo

Jaenisch

Wettstein³

1987