Mapping of the Human Testicular Proteome and its Relationship With That of the Epididymis and Spermatozoa

Li, Jianyuan; Liu, Fujun; Liu, Xin; Liu, Juan; Zhu, Ping; Wan, Feng-chun; Jin, Shao-Hua; Wang, Wenting; Li, Ning; Liu, Jie; Wang, Haiyan

doi:10.1074/mcp.m110.004630

Cited by 55 publications

(35 citation statements)

References 34 publications

(34 reference statements)

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“…36 Complementing this non-oxidative environment are anti-oxidant enzymes and components removing superoxide radicals and hydrogen peroxide, 37 some of which are reported in the human epididymal proteome. 38,39 These anti-oxidant strategies make excessive oxidative damage to epididymal cell DNA unlikely.…”

Section: Endogenous Protection Against Mutagenic Microenvironmentsmentioning

confidence: 99%

Why are epididymal tumours so rare?

Yeung¹,

Wang²,

Cooper³

2012

Asian J Androl

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Epididymal tumour incidence is at most 0.03% of all male cancers. It is an enigma why the human epididymis does not often succumb to cancer, when it expresses markers of stem and cancer cells, and constitutively expresses oncogenes, pro-proliferative and pro-angiogenic factors that allow tumour cells to escape immunosurveillance in cancer-prone tissues. The privileged position of the human epididymis in evading tumourigenicity is reflected in transgenic mouse models in which induction of tumours in other organs is not accompanied by epididymal neoplasia. The epididymis appears to: (i) prevent tumour initiation (it probably lacks stem cells and has strong anti-oxidative mechanisms, active tumour suppressors and inactive oncogene products); (ii) foster tumour monitoring and destruction (by strong immuno-surveillance and -eradication, and cellular senescence); (iii) avert proliferation and angiogenesis (with persistent tight junctions, the presence of anti-angiogenic factors and misplaced pro-angiogenic factors), which together (iv) promote dormancy and restrict dividing cells to hyperplasia. Epididymal cells may be rendered non-responsive to oncogenic stimuli by the constitutive expression of factors generally inducible in tumours, and resistant to the normal epididymal environment, which mimics that of a tumour niche promoting tumour growth. The threshold for tumour initiation may thus be higher in the epididymis than in other organs. Several anti-tumour mechanisms are those that maintain spermatozoa quiescent and immunologically silent, so the low incidence of cancer in the epididymis may be a consequence of its role in sperm maturation and storage. Understanding these mechanisms may throw light on cancer prevention and therapy in general.

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Section: Endogenous Protection Against Mutagenic Microenvironmentsmentioning

confidence: 99%

Why are epididymal tumours so rare?

Yeung¹,

Wang²,

Cooper³

2012

Asian J Androl

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“…Several studies have been done on luminal fluids released after mincing the tissue and by applying gentle pressure to the epididymal tubule [Girouard et al 2009;Li et al 2011;Zhang and Martin-Deleon 2003].…”

Section: Introductionmentioning

confidence: 99%

The contribution of proteomics to understanding epididymal maturation of mammalian spermatozoa

Dacheux

Belleannée

Guyonnet

et al. 2012

Systems Biology in Reproductive Medicine

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The acquisition of the ability of the male gamete to fertilize an ovum is the result of numerous and sequential steps of differentiation of spermatozoa that occur as they transit from the testis to the end of the epididymal tubule. The post gonadal sperm modifications are mostly related to motility, egg binding, and penetration processes. As the activity of the epididymis and its luminal fluid composition are believed to be directly related to 'sperm maturation', a review on epididymal proteins is presented. Comparative studies have shown that the epididymal activities are species specific. Nevertheless, for all mammalian species studied, similarities exist in the sequential proteomic changes of the luminal composition of the epididymal tubule and proteins on the sperm surface. The potential roles of these modifications are discussed.

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“…Peptide mixtures were analyzed using the 4800 Plus MALDI TOF/TOF TM Analyzer (ABI), which is a matrix-assisted laser desorption ionization time of flight (MALDI-TOF/TOF) mass spectrometer. Mass spectrometry was completed using an established method (Li et al, 2011). Proteins were identified using peak lists for searches against the NCBInr database with the Mascot search engine (http://www.matrixscience.com/).…”

Section: Protein Identification and Analysis Of Functionmentioning

confidence: 99%

Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress

Long¹,

Li²,

Zhang³

et al. 2019

The Model Legume Medicago Truncatula

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Salt stress is an important abiotic stress that causes decreased crop yields. Root growth and plant activities are affected by salt stress through the actions of specific genes that help roots adapt to adverse environmental conditions. For a more comprehensive understanding of proteins affected by salinity, we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. Our physiological and phenotypic observations indicated that Zhongmu-1 was more salt tolerant than Jemalong A17. We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively. Function analyses indicated molecule binding and catalytic activity were the two primary functional categories. These proteins have known functions in various molecular processes, including defense against oxidative stress, metabolism, photosynthesis, protein synthesis and processing, and signal transduction. The transcript levels of four identified proteins were determined by quantitative reverse transcription polymerase chain reaction. Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

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Mapping of the Human Testicular Proteome and its Relationship With That of the Epididymis and Spermatozoa

Cited by 55 publications

References 34 publications

Why are epididymal tumours so rare?

Why are epididymal tumours so rare?

The contribution of proteomics to understanding epididymal maturation of mammalian spermatozoa

Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress

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