Mapping of the Growth Hormone Gene by in situ Hybridization to Chicken Chromosome 1

Shaw, Emma; Shoffner, Robert N.; Foster, Douglas N.; Guise, K. S.

doi:10.1093/oxfordjournals.jhered.a111139

Cited by 14 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chicken genome sequence draft could be obtained from http://genome.ucsc.edu/cgi-bin/hgBlat and http://genome.wustl.edu/projects/ chicken/. By BLAST analysis, the locations of all 12 genes in the chromosomes were made clear, which was consistent with the original mapping results of some genes [10,16,32,34,42].…”

Section: Locating Genes On Chromosomessupporting

confidence: 67%

Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography

Nie¹,

Lei²,

Ouyang³

et al. 2005

Genet Sel Evol

View full text Add to dashboard Cite

Abstract

The genes that are part of the somatotropic axis play a crucial role in the regulation of growth and development of chickens. The identification of genetic polymorphisms in these genes will enable the scientist to evaluate the biological relevance of such polymorphisms and to gain a better understanding of quantitative traits like growth. In the present study, 75 pairs of primers were designed and four chicken breeds, significantly differing in growth and reproduction characteristics, were used to identify single nucleotide polymorphisms (SNP) using the denaturing high performance liquid chromatography (DHPLC) technology. A total of 283 SNP were discovered in 31 897 base pairs (bp) from 12 genes of the growth hormone (GH), growth hormone receptor (GHR), ghrelin, growth hormone secretagogue receptor (GHSR), insulin-like growth factor I and II (IGF-I and -II), insulin-like growth factor binding protein 2 (IGFBP-2), insulin, leptin receptor (LEPR), pituitary-specific transcription factor-1 (PIT-1), somatostatin (SS), thyroid-stimulating hormone beta subunit (TSH-β). The observed average distances in bp between the SNP in the 5'UTR, coding regions (non- and synonymous), introns and 3'UTR were 172, 151 (473 and 222), 89 and 141 respectively. Fifteen non-synonymous SNP altered the translated precursors or mature proteins of GH, GHR, ghrelin, IGFBP-2, PIT-1 and SS. Fifteen indels of no less than 2 bps and 2 poly (A) polymorphisms were also observed in 9 genes. Fifty-nine PCR-RFLP markers were found in 11 genes. The SNP discovered in this study provided suitable markers for association studies of candidate genes for growth related traits in chickens.

show abstract

Section: Locating Genes On Chromosomessupporting

confidence: 67%

Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography

Nie¹,

Lei²,

Ouyang³

et al. 2005

Genet Sel Evol

View full text Add to dashboard Cite

Abstract

show abstract

“…By BLAST analysis, the locations of all 12 genes in the chromosomes were made clear, which was consistent with the original mapping results of some genes [10,16,32,34,42].…”

Section: Locating Genes On Chromosomessupporting

confidence: 84%

Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography

Nie¹,

Lei²,

Ouyang³

et al. 2005

Genet. Sel. Evol.

View full text Add to dashboard Cite

-The genes that are part of the somatotropic axis play a crucial role in the regulation of growth and development of chickens. The identification of genetic polymorphisms in these genes will enable the scientist to evaluate the biological relevance of such polymorphisms and to gain a better understanding of quantitative traits like growth. In the present study, 75 pairs of primers were designed and four chicken breeds, significantly differing in growth and reproduction characteristics, were used to identify single nucleotide polymorphisms (SNP) using the denaturing high performance liquid chromatography (DHPLC) technology. A total of 283 SNP were discovered in 31 897 base pairs (bp) from 12 genes of the growth hormone (GH), growth hormone receptor (GHR), ghrelin, growth hormone secretagogue receptor (GHSR), insulin-like growth factor I and II (IGF-I and -II), insulin-like growth factor binding protein 2 (IGFBP-2), insulin, leptin receptor (LEPR), pituitary-specific transcription factor-1 (PIT-1), somatostatin (SS), thyroid-stimulating hormone beta subunit (TSH-β). The observed average distances in bp between the SNP in the 5'UTR, coding regions (non-and synonymous), introns and 3'UTR were 172, 151 (473 and 222), 89 and 141 respectively. Fifteen non-synonymous SNP altered the translated precursors or mature proteins of GH, GHR, ghrelin, IGFBP-2, PIT-1 and SS. Fifteen indels of no less than 2 bps and 2 poly (A) polymorphisms were also observed in 9 genes. Fifty-nine PCR-RFLP markers were found in 11 genes. The SNP discovered in this study provided suitable markers for association studies of candidate genes for growth related traits in chickens.chickens / genes / SNP / DHPLC * Corresponding author: xqzhang@scau.edu.cn 340 Q. Nie et al.

show abstract

“…Early in situ hybridization approaches located endogenous viral genes (ev) and several oncogenes to chromosomes (Tereba, 1983(Tereba, , 1985Bitgood and Somes, 1990). Shaw et al (1991) and Tereba et al (1991) have located the genes for growth hormone and aromatase, respectively, to the q arm of Chromosome 1. The newer fluorescent probe methods are now being used in chickens for the location of cloned genes by several groups (Soret et al, 1990;Dominguez-Steglisch et al, 1990, 1991, 1992aHutchison and LeCeil, 1992;Li and Ponce de Leon, 1992; N. J.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

et al. 1993

View full text Add to dashboard Cite

A reference population designed for molecular genetic mapping of the chicken genome was produced by backcrossing a partially inbred Red Jungle Fowl (JF) line to a highly inbred White Leghorn (WL) line. The parental lines were chosen to maximize the expected genetic polymorphisms between them. Two full-sib F^ males, produced by crossing a JF male with a WL female, were each individually mated to about 10 WL females to produce 400 progeny. All the progeny were classified for segregation of three loci controlling color phenotype and six blood group loci, some of which have been mapped by classical methods. Segregation of these nine loci did not differ significantly from the expected 1:1 ratio with one exception. At least 20 mL of whole blood was stored from all the parents and progeny to provide DNA for molecular analysis. Screening of the parental lines and Fi crosses by Southern blot with cloned genes and by the random amplified polymorphic DNA (RAPD) procedure revealed a large number of molecular markers mat were parental line-specific. A preliminary analysis of 16 backcross progeny classified for polymorphisms at 2 color loci, 6 blood group loci, 16 loci detected by cloned chicken genes, and 4 loci detected by the RAPD method has been completed. Segregation at 27 out of 28 loci did not differ significantly from the expected 1:1 ratio, showing that two alternative alleles were detected at each locus. Five pairs of linked loci were detected (P < .01). Thus, this population is polymorphic and gives simple segregation for two types of molecular probes, providing a good resource for collaborative mapping of the chicken genome. (

show abstract

Mapping of the Growth Hormone Gene by in situ Hybridization to Chicken Chromosome 1

Cited by 14 publications

References 0 publications

Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography

Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography

Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography

Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

Contact Info

Product

Resources

About