Mapping of Resistance to Vegetable Polyphenols among Aedes Taxa (Diptera, Culicidae) on a Molecular Phylogeny

Rey, Delphine; Després, Laurence; Schaffner, Francis; Meyran, Jean-Claude

doi:10.1006/mpev.2001.0950

Cited by 19 publications

(19 citation statements)

References 45 publications

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“…Multiplex PCR assays that included both universal (conserved) and species‐specific primers were performed by Phuc et al (2003). By contrast with the many studies on nuclear genes, little taxonomic work has targeted haploid mitochondrial DNA sequences in mosquitoes and less yet has examined sequence diversity in the CO1 gene (Rey et al , 2001; Fairley et al , 2000, 2002; Sallum et al, 2002), despite its established potential for the diagnosis of biological diversity (Hebert et al , 2003a, 2003b, 2004). The CO1 region is present in the hundreds of copies per cell, it generally lacks indels, and, in common with other protein‐coding genes, its third position nucleotides show a high incidence of base substitutions.…”

Section: Introductionmentioning

confidence: 99%

Identifying Canadian mosquito species through DNA barcodes

Cywinska

Hunter

2006

Medical Vet Entomology

291

231

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A short fragment of mt DNA from the cytochrome c oxidase 1 (CO1) region was used to provide the first CO1 barcodes for 37 species of Canadian mosquitoes (Diptera: Culicidae) from the provinces Ontario and New Brunswick. Sequence variation was analysed in a 617-bp fragment from the 5' end of the CO1 region. Sequences of each mosquito species formed barcode clusters with tight cohesion that were usually clearly distinct from those of allied species. CO1 sequence divergences were, on average, nearly 20 times higher for congeneric species than for members of a species; divergences between congeneric species averaged 10.4% (range 0.2-17.2%), whereas those for conspecific individuals averaged 0.5% (range 0.0-3.9%).

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Section: Introductionmentioning

confidence: 99%

Identifying Canadian mosquito species through DNA barcodes

Cywinska

Hunter

2006

Medical Vet Entomology

291

231

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“…With one exception (i.e. Rey et al ., 2001), Reinert et al . (2004, 2006) reviewed the results of molecular (DNA) studies of aedine taxa published up to the end of 2005 (i.e.…”

Section: Introductionmentioning

confidence: 99%

Phylogeny and classification of Ochlerotatus and allied taxa (Diptera: Culicidae: Aedini) based on morphological data from all life stages

2008

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The phylogenetic relationships and generic assignments of 'Ochlerotatus' and related taxa of uncertain taxonomic position in the classification of Aedini previously proposed by the authors in 2004 and 2006 are explored using 297 characters from eggs, fourth-instar larvae, pupae, adults and immature habitat coded for 158 exemplar species. The ingroup comprises 54 species and the outgroup includes four non-aedine species and 100 aedine species, 21 of which were previously classified as incertae sedis. Data are analysed in a total-evidence approach using implied weighting. The analysis produced 158 most parsimonious cladograms. The strict consensus tree (SCT) corroborates the monophyly of the 30 generic-level taxa recognized previously that are included in the analysis. Overall, the results show remarkable congruence with those obtained previously despite differences in the taxa and morphological characters analysed in this and the two previous studies. All species of Ochlerotatus s.s., subgenus 'Ochlerotatus' sensu auctorum, Geoskusea, Levua, Pseudoskusea and Rhinoskusea included in the analysis fall within a single clade that is treated as genus Ochlerotatus; thus, the last four taxa are restored to their previous subgeneric rank within this genus. Nine additional subgenera, of which four are new, are proposed for monophyletic clades of Ochlerotatus species based on the strength of character support and application of the principle of equivalent rank. Acartomyia stat. nov., Culicelsa stat. nov., Gilesia stat. nov., Protoculex stat. nov. and Chrysoconops stat. nov. are resurrected from synonymy with Ochlerotatus; and Empihals subgen. nov. (type species: Culex vigilax Skuse), Pholeomyia subgen. nov. (type species: Aedes calcariae Marks), Buvirilia subgen. nov. (type species: Aedes edgari Stone & Rosen) and Sallumia subgen. nov. (type species: Aedes hortator Dyar & Knab) are described as new. The sister group of Ochlerotatus includes a number of species that were previously regarded as incertae sedis in 'Oc. (Finlaya)' and 'Oc. (Protomacleaya)'. Based on previous observations, refined relationships and new character support, three additional genera are recognized for species previously included in 'Finlaya', i.e. Danielsia stat. nov. (type species: Danielsia albotaeniata Leicester), Luius gen. nov. (type species: Aedes fengi Edwards) and Hopkinsius gen. nov. (type species: Aedes ingrami Edwards). Additionally, Alloeomyia subgen. nov. (type species: Culex pseudotaeniatus Giles) and Yamada subgen. nov. (type species: Aedes seoulensis Yamada) are introduced as subgenera of Collessius and Hopkinsius, respectively. As is usual with generic-level groups of Aedini, the newly recognized genera and subgenera are polythetic taxa that are diagnosed by unique combinations of characters. The analysis corroborates the previous observation that 'Oc. (Protomacleaya)' is a polyphyletic assemblage of species.

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“…Ae. communis is the most common and widely distributed mosquito species in northern Sweden [11], and is frequently found in other parts of Sweden as well [12, 36, 37]. Genetic barcoding of mosquitoes by sequencing the COI gene is useful for determining the species of potential viral vectors, and their future implication in the transmission and dissemination of arboviruses [12].…”

Section: Discussionmentioning

confidence: 99%

Mosquito-borne Inkoo virus in northern Sweden - isolation and whole genome sequencing

Lwande

Bucht

Ahlm

et al. 2017

Virol J

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BackgroundInkoo virus (INKV) is a less known mosquito-borne virus belonging to Bunyaviridae, genus Orthobunyavirus, California serogroup. Studies indicate that INKV infection is mainly asymptomatic, but can cause mild encephalitis in humans. In northern Europe, the sero-prevalence against INKV is high, 41% in Sweden and 51% in Finland. Previously, INKV RNA has been detected in adult Aedes (Ae.) communis, Ae. hexodontus and Ae. punctor mosquitoes and Ae. communis larvae, but there are still gaps of knowledge regarding mosquito vectors and genetic diversity. Therefore, we aimed to determine the occurrence of INKV in its mosquito vector and characterize the isolates.MethodsAbout 125,000 mosquitoes were collected during a mosquito-borne virus surveillance in northern Sweden during the summer period of 2015. Of these, 10,000 mosquitoes were processed for virus isolation and detection using cell culture and RT-PCR. Virus isolates were further characterized by whole genome sequencing. Genetic typing of mosquito species was conducted by cytochrome oxidase subunit I (COI) gene amplification and sequencing (genetic barcoding).ResultsSeveral Ae. communis mosquitoes were found positive for INKV RNA and two isolates were obtained. The first complete sequences of the small (S), medium (M), and large (L) segments of INKV in Sweden were obtained. Phylogenetic analysis showed that the INKV genome was most closely related to other INKV isolates from Sweden and Finland. Of the three INKV genome segments, the INKV M segment had the highest frequency of non-synonymous mutations. The overall G/C-content of INKV genes was low for the N/NSs genes (43.8–45.5%), polyprotein (Gn/Gc/NSm) gene (35.6%) and the RNA polymerase gene (33.8%) This may be due to the fact that INKV in most instances utilized A or T in the third codon position.ConclusionsINKV is frequently circulating in northern Sweden and Ae. communis is the key vector. The high mutation rate of the INKV M segment may have consequences on virulenceElectronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0725-5) contains supplementary material, which is available to authorized users.

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Mapping of Resistance to Vegetable Polyphenols among Aedes Taxa (Diptera, Culicidae) on a Molecular Phylogeny

Cited by 19 publications

References 45 publications

Identifying Canadian mosquito species through DNA barcodes

Identifying Canadian mosquito species through DNA barcodes

Phylogeny and classification of Ochlerotatus and allied taxa (Diptera: Culicidae: Aedini) based on morphological data from all life stages

Mosquito-borne Inkoo virus in northern Sweden - isolation and whole genome sequencing

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