Mapping of QTL and identification of candidate genes conferring spontaneous haploid genome doubling in maize (Zea mays L.)

Ren, Jiaojiao; Boerman, Nicholas A.; Liu, Ruixiang; Wu, Penghao; Trampe, Benjamin; Vanous, Kimberly; Frei, Ursula; Chen, Shaojiang; Lübberstedt, Thomas

doi:10.1016/j.plantsci.2019.110337

Cited by 25 publications

(38 citation statements)

References 51 publications

(72 reference statements)

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“…Moreover, in Chinese germplasm, Yang et al [38] mapped nine QTL in total ( Figure 2), one of which was located in bin 3.05 on chromosome 3 near qhmf2 described by Ren et al [23], and three QTL in bins 7.01 and 7.02 flanked a significant SNP on chromosome 7 ( Figure 2) that was identified by Chaikam et al [34]. Using a similar approach to [23], Ren et al [21] mapped a large-effect QTL (qshgd1) to chromosome 5 in US-derived germplasm, and a small-effect QTL (qshgd2) to chromosome 6, which mapped to a different location than qhmf4 ( Figure 2).…”

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 90%

“…Recently, five inbred lines exhibiting haploid male fertility (HMF) greater than 65% without the use of colchicine were identified by their pollen-shedding ability and proportion of anther emergence [20]. Furthermore, a single large-effect QTL has been identified on chromosome 5 in the inbred that had the highest level of HMF, at 94% [21,22]. Interestingly, this QTL is different from that identified on chromosome 6 within an inbred developed in China that also possesses a high degree of HMF [23].…”

Section: History and Uses Of Dh Technologymentioning

confidence: 99%

“…[23], Ren et al [21] mapped a large-effect QTL (qshgd1) to chromosome 5 in US-derived germplasm, and a small-effect QTL (qshgd2) to chromosome 6, which mapped to a different location than qhmf4 ( Figure 2). Furthermore, linkage mapping within a bi-parental mapping population using the same high SHGD parent as was used by Ren et al [21] identified a large-effect QTL possessing additive gene action in the same region as qshgd1 ( Figure 2) [22].…”

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 99%

“…Possible candidate genes for large-effect SHGD QTL have been suggested [21,23]. The gene absence of first division (afd1) is characterized by having an absence of first division during meiosis Furthermore, linkage mapping within a bi-parental mapping population using the same high SHGD parent as was used by Ren et al [21] identified a large-effect QTL possessing additive gene action in the same region as qshgd1 ( Figure 2) [22]. Similarly, two small-effect QTL in the same regions as qshgd2 and qshgd3 identified by Ren et al [21] were also detected ( Figure 2) [22].…”

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 99%

“…The gene absence of first division (afd1) is characterized by having an absence of first division during meiosis Furthermore, linkage mapping within a bi-parental mapping population using the same high SHGD parent as was used by Ren et al [21] identified a large-effect QTL possessing additive gene action in the same region as qshgd1 ( Figure 2) [22]. Similarly, two small-effect QTL in the same regions as qshgd2 and qshgd3 identified by Ren et al [21] were also detected ( Figure 2) [22]. These differing QTL between the Chinese and US germplasms indicate that there are likely multiple mechanisms underlying similar phenotypes, possibly depending on the origin of germplasm.…”

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 99%

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Impact of Spontaneous Haploid Genome Doubling in Maize Breeding

Boerman

Frei

Lübberstedt

2020

Plants

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Doubled haploid (DH) technology has changed the maize-breeding landscape in recent years. Traditionally, DH production requires the use of chemical doubling agents to induce haploid genome doubling and, subsequently, male fertility. These chemicals can be harmful to humans and the plants themselves, and typically result in a doubling rate of 10%-30%. Spontaneous genome doubling and male fertility of maize haploids, without using chemical doubling agents, have been observed to a limited extent, for nearly 70 years. Rates of spontaneous haploid genome doubling (SHGD) have ranged from less than 5% to greater than 50%. Recently, there has been increased interest to forgo chemical treatment and instead utilize this natural method of doubling. Genetic-mapping studies comprising worldwide germplasm have been conducted. Of particular interest has been the detection of large-effect quantitative trait loci (QTL) affecting SHGD. Having a single large-effect QTL with an additive nature provides flexibility for the method of introgression, such as marker-assisted backcrossing, marker-assisted gene pyramiding, and systematic design. Moreover, it allows implementation of new methodologies, such as haploid-inducer mediated genome editing (HI-edit) and promotion of alleles by genome editing. We believe the use of SHGD can further enhance the impact of DH technology in maize.

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Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 90%

Section: History and Uses Of Dh Technologymentioning

confidence: 99%

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 99%

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 99%

Section: Genetic Architecture and Candidate Genes Of Spontaneous Haplmentioning

confidence: 99%

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Impact of Spontaneous Haploid Genome Doubling in Maize Breeding

Boerman

Frei

Lübberstedt

2020

Plants

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A diallel analysis of a maize donor population response to in vivo maternal haploid induction: II. Haploid male fertility

et al. 2020

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Doubled haploid (DH) lines are used in maize (Zea mays L.) breeding to accelerate the breeding cycle and create homogenous inbred lines in as little as two seasons. These pure inbred lines allow breeders to quickly evaluate new cross combinations. There are two important steps in creating DH lines: (a) generation and selection of haploid progeny, and (b) genome doubling to create fertile, diploid inbreds. Colchicine is widely used to artificially double genomes in haploid plants, which is hazardous, expensive, and time consuming. In this study, three public inbred lines-A427, A637, and NK778-were found to have substantial haploid male fertility (HMF). A sixparent full diallel between these three HMF lines and three non-HMF lines was created and HMF was scored. Diallel analysis revealed significant general combining ability (GCA) estimates of up to 17% for HMF, as well as significant specific combining ability (SCA) effects of up to 25%. No significant reciprocal effects were found. Haploid male fertility is promising to be incorporated into elite maize breeding programs to potentially overcome the need of using colchicine treatments for genome doubling. Colchicine aided doubling success rates varying from almost 0 to 30%. Haploid male fertility has an advantage over artificial genome doubling, in terms of both increased success rates and decreased costs for DH line production.

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