Mapping of microsatellite loci and association of aorta atherosclerosis with LG VI markers in the rabbit

Korstanje, Ron; Gillissen, Gert; Kodde, Linda; Bieman, M. Den; Lankhorst, A.; Zutphen, L.F.M. van; Lith, H.A. van

doi:10.1152/physiolgenomics.2001.6.1.11

Cited by 11 publications

(11 citation statements)

References 25 publications

(18 reference statements)

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“…These microsatellites were previously shown to be genetically linked to the C-locus and Est-5, which are assigned to linkage group 1 on OCU1 (Korstanje et al, 2001a). Our results confirm the localization of LGI on OCU1.…”

Section: Resultssupporting

confidence: 88%

“…The mapping of NPT2 to OCU3p and of PMP2 to OCU3q is in agreement with the comparative painting data (see Table 3). The microsatellite located within PMP2, Sat3, together with the anonymous microsatellite Sol33 and the ileal lipid binding protein gene (FABP6) form linkage group XI (Korstanje et al 2001a). In the present study we mapped Sol33 just proximal to PMP2 on OCU3q11 (Fig.…”

Section: Resultsmentioning

confidence: 75%

“…This suggests that MT1 is only syntenic with CASR-rs1 and CASR-rs2 in the rabbit. The microsatellite contained within MT1 has been assigned to linkage group VI (Korstanje et al, 2001a). This linkage group further consists of several esterase genes and haptoglobin (Korstanje et al, 2001a(Korstanje et al, , 2001c.…”

Section: Resultsmentioning

confidence: 99%

“…In order to assess the coverage of individual chromosomes by linkage groups, and to orient linkage groups along chromosomes, it is necessary to map genetic markers belonging to specific linkage groups on metaphase chromosomes. In the present study we describe the chromosomal localization of microsatellite markers that have recently been used for linkage studies (Korstanje et al, 2001a(Korstanje et al, , 2001b, thus providing links between the genetic and the physical maps of the rabbit genome. For the localization of these markers we used the DNA of clones from a rabbit Bacterial Artificial Chromosome (BAC) library as probe for FISH.…”

confidence: 99%

“…Positive clones were cultured and DNA was isolated according to standard procedures. In order to verify the presence of the microsatellite sequences of interest in the selected clones, PCR was performed on the isolated BAC DNA as previously described (Korstanje et al, 2001a(Korstanje et al, , 2001b, and the amplification products were separated on a 3 % MS-8 pronarose (Sphearo Q) gel. Table 2 lists the names of the genes containing the respective microsatellites, the sequences of the primers used, the mean size of the amplification products, the primer references and the EMBL accession numbers for the complete sequences.…”

Section: Fish Probesmentioning

confidence: 99%

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Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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In order to improve the informativeness of the cytogenetic map of the rabbit genome, fourteen markers were regionally mapped to individual chromosomes. The localizations comprise eleven gene loci (PRLR, GHR, HK1, ACE, TF, 18S+28S rDNA, CYP2C4, PMP2, TCRB, ALOX15 and MT1) and three microsatellite loci (Sat13, Sol33 and D1Utr6). Five of the genes contain known microsatellite sequences. To achieve these localizations, homologous and heterologous small insert clones, and clones from a rabbit Bacterial Artificial Chromosome (BAC) library were used as probes for fluorescence in situ hybridization experiments. Results indicate that especially BAC clones are a valuable tool for cytogenetic mapping. Some of the genes were selected for mapping on the basis of human- rabbit comparative painting data, to achieve localizations on gene-poor rabbit chromosomes. Our data are, in general, in agreement with the human-rabbit comparative painting data. By mapping microsatellite sequences that have also been used in linkage studies, links are provided between the genetic and physical maps of the rabbit genome. Linkage groups I, VI and XI could be assigned to chromosomes 1, 5 and 3 respectively. Moreover, in this paper we give an overview of the current status of the rabbit cytogenetic map. This map now comprises 62 physically mapped genes, which are scattered over all autosomes, except chromosome 2, and the X chromosome.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Fish Probesmentioning

confidence: 99%

See 3 more Smart Citations

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus)

Beugin

Letty

Kaerle³

et al. 2017

Ecology and Evolution

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The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Nongenetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with noninvasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totaling 126 alleles, and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares.

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Genome Mapping and Genomics in Domestic Animals

Cockett¹,

Kole²

2009

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Mapping of microsatellite loci and association of aorta atherosclerosis with LG VI markers in the rabbit

Cited by 11 publications

References 25 publications

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus)

Genome Mapping and Genomics in Domestic Animals

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