Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Abstract

In order to improve the informativeness of the cytogenetic map of the rabbit genome, fourteen markers were regionally mapped to individual chromosomes. The localizations comprise eleven gene loci (PRLR, GHR, HK1, ACE, TF, 18S+28S rDNA, CYP2C4, PMP2, TCRB, ALOX15 and MT1) and three microsatellite loci (Sat13, Sol33 and D1Utr6). Five of the genes contain known microsatellite sequences. To achieve these localizations, homologous and heterologous small insert clones, and clones from a rabbit Bacterial Artificial Chromosome (BAC) library were used as probes for fluorescence in situ hybridization experiments. Results indicate that especially BAC clones are a valuable tool for cytogenetic mapping. Some of the genes were selected for mapping on the basis of human- rabbit comparative painting data, to achieve localizations on gene-poor rabbit chromosomes. Our data are, in general, in agreement with the human-rabbit comparative painting data. By mapping microsatellite sequences that have also been used in linkage studies, links are provided between the genetic and physical maps of the rabbit genome. Linkage groups I, VI and XI could be assigned to chromosomes 1, 5 and 3 respectively. Moreover, in this paper we give an overview of the current status of the rabbit cytogenetic map. This map now comprises 62 physically mapped genes, which are scattered over all autosomes, except chromosome 2, and the X chromosome.

Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes

“…However, we localized CALR on OCU10 and GHR on OCU11. As previously discussed by Zijlstra et al (2002), we confirm that OCU10 and OCU11 are interchanged in the study by Korstanje et al (1999). Concerning DNASE1, located on HSA16p13.3, we have localized it on the p arm of OCU6 and not on the q arm as expected from Korstanje and co-workers (1999).…”

“…IGF2, TGFA, IGF1, DNASE1, F7, CYB5, CALR, SLC2A1, AHSG, PIGR, LIPC, TGFB3, NOS1 and SRY. Eight genes, TNF (Rogel-Gaillard et al, 2001), CSN1 (Pauloin et al, 2002), PMP2, MT1A, TRB GHR, CYP2C4 and ALOX15 (Zijlstra et al, 2002), have been previously mapped and their localization is confirmed here on either G-or Rbanded chromosomes. Finally the HPRT1 gene, which had been previously assigned to OCUX by somatic cell hybrid analysis (Echard et al, 1981), is now regionally mapped to Xq23.…”

“…After capturing, slides were washed twice in 4× SSC, 0.05 % Tween 20, dehydrated and air-dried. Fluorescent in situ hybridization was performed with biotin-16-dUTP nick-translated probes as described by Zijlstra et al (2002). Previously captured metaphases were reexamined after FISH, recaptured, and analyzed using the GENUS software.…”

“…To date, the number of genes cytogenetically localized in the rabbit is small, and in addition, chromosome 2 lacks any mapped gene and chromosomes 10 and 21 have only one mapped gene with no known human ortholog (Zijlstra et al, 2002). Therefore, we defined a Fig.…”

Genome Mapping and Genomics in Domestic Animals

“…Later, chromosomal analyses using cultured cells (Nichols et al 1965;Ray and Williams 1966;Issa et al 1968) confirmed the pioneering observations made by Painter. Finally, it has been shown that chromosomes 13, 16, and 20 (telomeric p arm) and 21 (telomeric q arm) bear the nucleolar organizer regions (Martin-DeLeon 1980;Zijlstra et al 2002). The banding patterns of rabbit chromosomes have been described in detail by several groups: G-banded metaphase chromosomes (Echard 1973;Chan et al 1977;Ford et al 1980; Committee for Standardized Karyotype of Oryctolagus cuniculus 1981), G-, Q-, and R-banded metaphase chromosomes (Hageltorn and Gustavsson 1979), G-banded late prophase chromosomes (Yerle et al 1987), R-banded prometaphase chromosomes (Poulsen et al 1988;Rønne et al 1993, Rønne 1995, and G-and R-banded midmetaphase chromosomes .…”