Mapping of functional domains and characterization of the transcription factor Cph1 that mediate morphogenesis in Candida albicans

Maiti, Protiti; Ghorai, Priyanka; Ghosh, Sumit; Kamthan, Mohan; Tyagi, Rakesh K.; Datta, Asis

doi:10.1016/j.fgb.2015.08.004

Cited by 21 publications

(16 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The capacity to form biofilms has been associated with the presence of transcriptional regulatory genes in C. albicans and these include the transcriptional regulators EFG1, BCR1, TEC1, NDT80, and ROB1 (Maiti et al 2015;Gulati and Nobile 2016). Hyphal formation contributes to the overall architectural stability of the biofilm and acts as a support for yeast cells, pseudohyphae and other the control group was observed for both groups, with a reduction of 57.5% for biofilms associated with probiotic cells and 45.1% for the corresponding supernatant.…”

Section: Discussionmentioning

confidence: 99%

“…The ALS3 gene encoding a protein similar to alphaagglutinin plays an essential role in the adhesion of C. albicans and serves as an upstream regulator of both the Ras1-cAMP-Efg1 and MAPK pathways (Maiti et al 2015). CPH1 can act in concert with another regulator of morphogenesis, EFG1, the transcription factor of the cAMP-PKA pathway.…”

Section: Disclosure Statementmentioning

confidence: 99%

“…The cph1efg1Δ double mutant exhibited loss of function of almost the entire transcription machinery that regulates filamentation, and the mutant was avirulent in a candidiasis mouse model (Lo et al 1997). Recently, CPH1 has been implicated in the maintenance of cell wall organization, pseudohyphal formation in response to oxidative stress, biofilm formation and in regulation of the pheromone response in both white and opaque phase cells (Morschhauser 2011;Srinivasa et al 2012;Maiti et al 2015). The YWP1 gene plays a key role in adhesion and dispersal of the yeast form in biofilms.…”

Section: Disclosure Statementmentioning

confidence: 99%

See 2 more Smart Citations

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

et al. 2018

View full text Add to dashboard Cite

This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Disclosure Statementmentioning

confidence: 99%

Section: Disclosure Statementmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The genes EFG1 and TEC1 are transcription factors and are related to morphogenesis, which form a regulating circuit of hyphal differentiation [30]. CPH1 is a transcription factor and regulates the morphogenesis of C. albicans [32]. The BCR1 gene is among the major biofilm regulators [37].…”

Section: Discussionmentioning

confidence: 99%

Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms

Freire

Barros²,

Pereira³

et al. 2018

Lasers Med Sci

View full text Add to dashboard Cite

The objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time. The photosensitizer methylene blue at 300 uM and erythrosine at 400 uM, sensitized with low-power laser (visible red, 660 nm) and green LED (532 nm), respectively, were used for PDI. Four groups of each sample and PDI protocol were evaluated: (a) P+L+: sensitization with the photosensitizer and irradiation with light, (b) P+L-: only treatment with the photosensitizer, (c) P-L+: only irradiation with light, and (d) P-L-: without sensitization with the dye and absence of light. The results were analyzed by t test, with a significance level of 5%. The photodynamic inactivation was able to reduce the expression of all genes for both treatments, laser and LED. The fold-decrease for the genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 were 0.73, 0.39, 0.77, 0.71, 0.67, and 0.60 for laser, respectively, and 0.66, 0.61, .050, 0.43, 0.54, and 0.66 for LED, respectively. It could be concluded that PDI showed a reduction in the expression of C. albicans genes, suggesting its virulence decrease.

show abstract

“…Cph1 is a transcription factor acting downstream of Cek1 during hyphal morphogenesis under some conditions (Liu et al, 1994;Desai et al, 2015), which has been described to downregulate CEK1, CST20, HST7 and STE11 genes under oxidative stress (Maiti et al, 2015). To explore the role of Cph1 in glycostress signaling, transcript levels of relevant genes were examined in a cph1 mutant strain.…”

Section: Ace2 Regulates Cek1 Pathway Genes Under Glycostressmentioning

confidence: 99%

Candida albicans responds to glycostructure damage by Ace2‐mediated feedback regulation of Cek1 signaling

Wijlick

Swidergall

Brandt

et al. 2016

Molecular Microbiology

View full text Add to dashboard Cite

Candida albicans uses the Cek1 MAPK pathway to restore cells from damage of its cell wall glycostructures. Defective protein N- or O-glycosylation activates Cek1 and the transcription factor Ace2 as its downstream target, to upregulate genes encoding protein O-mannosyltransferases (Pmt proteins). In unstressed cells, Cek1-Ace2 activity blocks expression of PMT1, which is de-repressed by tunicamycin. Genomic binding targets of Ace2 included ZCF21, which was upregulated by Ace2 and found to repress PMT1 transcription in unstressed cells. Surprisingly, genes encoding components of the Cek1 pathway including MSB2, CST20, HST7, CEK1 and ACE2 were also identified as Ace2 targets indicating Ace2-mediated transcriptional amplification of pathway genes under N-glycosylation stress. In this condition, physical interaction of the Ace2 protein with the upstream MAPKKK Cst20 was detected. Cst20-GFP showed stress-induced import from the cytoplasm into the nucleus and phosphorylation of Ace2. Interestingly, forced nuclear localization of Cst20 inhibited Cek1-Ace2 signaling, while forced cytoplasmic localization of Cst20 retained full signaling activity, suggesting that nuclear Cst20 downregulates the Cek1 pathway. Collectively, the results indicate that Ace2 is a versatile multifunctional transcriptional regulator, which activates glycostress responses of C. albicans by both positive forward and negative feedback regulation of Cek1 signaling.

show abstract

Mapping of functional domains and characterization of the transcription factor Cph1 that mediate morphogenesis in Candida albicans

Cited by 21 publications

References 50 publications

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms

Candida albicans responds to glycostructure damage by Ace2‐mediated feedback regulation of Cek1 signaling

Contact Info

Product

Resources

About