Antifungal activity of clinical <i>Lactobacillus</i> strains against <i>Candida albicans</i> biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Rossoni, Rodnei Dennis; Barros, Patrícia Pimentel de; Alvarenga, Janaína Araújo de; Ribeiro, Felipe de Camargo; Velloso, Marisol dos Santos; Fuchs, Beth Burgwyn; Mylonakis, Eleftherios; Jorge, Antônio Olavo Cardoso

doi:10.1080/08927014.2018.1425402

Cited by 75 publications

(62 citation statements)

References 61 publications

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“…The use of this biosurfactants could be developed further as a potential new coating material for medical devices to minimize Candida infections [134]. In general, supernatants of different Lactobacillus species were found to reduce adhesion of C. albicans to HeLa cells (L. crispatus, L. gasseri) [125], to plastic surface (L. paracasei) [135], as well as to TR146 cells (L. rhamnosus) [136].…”

Section: Other Antifungal Factorsmentioning

confidence: 99%

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The role of Lactobacillus species in the control of Candida via biotrophic interactions

et al. 2020

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Microbial communities have an important role in health and disease. Candida spp. are ubiquitous commensals and sometimes opportunistic fungal pathogens of humans, colonizing mucosal surfaces of the genital, urinary, respiratory and gastrointestinal tracts and the oral cavity. They mainly cause local mucosal infections in immune competent individuals. However, in the case of an ineffective immune defense, Candida infections may become a serious threat. Lactobacillus spp. are part of the human microbiome and are natural competitors of Candida in the vaginal environment. Lactic acid, low pH and other secreted metabolites are environmental signals sensed by fungal species present in the microbiome. This review briefly discusses the ternary interaction between host, Lactobacillus species and Candida with regard to fungal infections and the potential antifungal and fungistatic effect of Lactobacillus species. Our understanding of these interactions is incomplete due to the variability of the involved species and isolates and the complexity of the human host.

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Section: Other Antifungal Factorsmentioning

confidence: 99%

“…influence C. albicans morphology. Hyphae formation was impaired in co-culture with L. paracasei [120,135]. Interaction of Lactobacillus and C. albicans alters the gene expression pattern towards the yeast form.…”

Section: Other Antifungal Factorsmentioning

confidence: 99%

The role of Lactobacillus species in the control of Candida via biotrophic interactions

et al. 2020

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“…In this study, we used Streptococcus mutans UA159 and 22 clinical strains of Lactobacillus that were previously isolated from the oral cavities of caries-free subjects, which were identified by an API 50 CHL system (BioMérieux, France) and confirmed by PCR. These Lactobacillus strains showed great potential to inhibit C. albicans strains from the oral cavity in vitro and to negatively modulate virulence gene expression [16]. This study was approved by the Human Research Ethics Committee of the Institute of Science and Technology, Univ Estadual Paulista/UNESP (protocol number 754.634).…”

Section: Microorganisms and Growth Conditionsmentioning

confidence: 99%

“…After biofilm formation, the biofilm biomass was quantified utilizing an assay previously described by Rossoni et al [16] and Peeters et al [17], with modifications. For fixation of the biofilms, 100 μl of 99% methanol was added to the wells (Sigma-Aldrich, São Paulo, Brazil).…”

Section: Antibacterial Activity Of Lactobacillus Supernatants On S Mmentioning

confidence: 99%

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Rossoni

Velloso

Barros

et al. 2018

Microbial Pathogenesis

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Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.

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“…например, получены данные, что изоляты L. paracasei, L. rhamnosus и L. fermentum проявляют выраженные ингибирующие свойства в отношении C. albicans. совместная инкубация приводила к задержке формирования биопленки и замедлению образования псевдомицелия [14].…”

Section: материалы и методыunclassified

In vitro antimycotic sensitivity of the genus candida yeast and lactobacillus rhamnosus probiotic strain

Spasibova¹,

Владимировна²,

Savicheva³

et al. 2019

Z.akus.zen.bolezn

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Hypothesis/aims of study. Vulvovaginal candidiasis (VVC) is one of the most common inflammatory diseases of the vagina. Herewith, a synergistic effect of lactobacilli and antifungal drugs in VVC treatment is suggested. In this regard, it seems relevant to study the effect of antimycotics used to treat VVC on the enhanced L. casei rhamnosus 35 (LCR35) probiotic strain. This study was aimed to determine the sensitivity to antimycotic drugs of the probiotic LCR35 strain included in the Lactoginal vaginal capsules, as well as the sensibility of the genus Candida yeast under separate and joint cultivation with lactobacilli. Study design, materials and methods. LCR35 was cultured on a MRS agar. Identification of bacteria grown on the medium, as well as clinical isolates of C. albicans and C. parapsilosis, isolated from the vagina, was performed by MALDI-TOF mass spectrometry. The sensitivity of yeast was determined on a modified Mller-Hinton agar with 40% glucose and methylene blue. Discs with fluconazole, voriconazole, itraconazole, ketoconazole, clotrimazole, and nystatin were used. Interpretation of growth inhibition zones was carried out according to EUCAST-2018 or according to the recommendations of the disc manufacturer. Results. The probiotic LCR35 strain is resistant to antimycotics that are effective against C. albicans and C. parapsilosis. When determining the sensibility of C. albicans and C. parapsilosis to antimycotics in separate and joint cultivation with LCR35, no differences were found in the degree of yeast-like fungi growth suppression. Conclusion. The probiotic LCR35 strain, which is part of the Lactoginal vaginal capsules, is resistant to antimycotic drugs and does not affect the sensitivity of the tested yeast-like fungi to antimycotics.

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Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Cited by 75 publications

References 61 publications

The role of Lactobacillus species in the control of Candida via biotrophic interactions

The role of Lactobacillus species in the control of Candida via biotrophic interactions

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

In vitro antimycotic sensitivity of the genus candida yeast and lactobacillus rhamnosus probiotic strain

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