Mapping linear B-cell epitopes of the Tryparedoxin Peroxidase and its implications in the serological diagnosis of tegumentary leishmaniasis

Medeiros, Rutyanne Maria Tonelli Elisei; Carvalho, Ana Maria Ravena Severino; Ferraz, Isabela de Andrade; Medeiros, Fernanda Alvarenga Cardoso; Cruz, Luiza dos Reis; Silva, Manoel Otávio da Rocha; Coelho, Eduardo Antônio Ferraz; Gonçalves, Denise Utsch; Mendes, Tiago; Duarte, Mariana C.; Menezes‐Souza, Daniel

doi:10.1016/j.actatropica.2022.106521

Cited by 4 publications

(11 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 82 ], in which the cases were selected from a health service or hospital. To assess the performance of peptide-based tests, three different formats were reported: one study used an ICT format [ 7 ], two studies used phage-ELISA [ 15 , 62 ] and 19 studies used an ELISA technique [ 10 , 11 , 16 , 17 , 26 , 27 , 29 , 35 , 37 , 40 – 43 , 47 , 48 , 58 , 63 , 69 , 70 ]. In these studies using an ELISA method, a marked difference was observed in the amount of peptides used, which ranged from 0.25 μg to 20 μg per well.…”

Section: Resultsmentioning

confidence: 99%

“…et al ., 2017 [ 35 ] P1 Parasitology ELISA 0.25 μg/well I CL Brazil L. braziliensis P2 P3 Mix P1+P2+P3 Machado, A.S. et al ., 2020 [ 37 ] PeptC Molecular technique (VL patients); serology (control group) ELISA 10 μg/well II VL Brazil L. infantum Medeiros et al . 2022 [ 40 ] peptide Parasitology, molecular technique ELISA 1 μg/well II TL Brazil L. braziliensis Menezes-Souza, D. et al ., 2014 [ 42 ] Peptide 1 Parasitology, molecular technique ELISA 10 μg/well II TL Brazil L. braziliensis (TL); L. infantum (VL) VL Peptide 2 TL …”

Section: Resultsmentioning

confidence: 99%

“…A total of 77 different peptide sequences were tested as potential candidates for diagnostic tests ( Table 2 ), ranging from seven to 32 amino acids ( Supplementary data 2 ) in immunoassays, and performance data were collected. In all, 57 peptides were derived from 24 different proteins with identified accessions ( Table 2 ) such as leishmanolysin (gp63) [ 35 ], amastin [ 69 ], HSP83.1 [ 42 ], A2, LACK, NH [ 17 ], Histone H1 [ 10 ], β-tubulin [ 16 ] or tryparedoxin [ 40 , 70 ]. For the remaining 20 peptides, the protein was not known or was not identified (identification number (ID UniProt ( https://www.uniprot.org/ ) or NCBI accession number ( https://www.ncbi.nlm.nih.gov/protein/ ) are not specified) [ 15 , 17 , 58 , 62 ].…”

Section: Resultsmentioning

confidence: 99%

“…et al ., 2017 [ 35 ] GHRMPPTSVSALARP P1 GP63 XP_001562922.1 TMVPKEPNPLSGLRK P2 SKPQPNNFKLNSLGS P3 Mix P1+P2+P3 Mix P1+P2+P3 Machado, A.S. et al ., 2020 [ 37 ] KWKTGAALDGAPQPLNTL PeptC Hypothetical protein LiHyC XP_001470432.1 Medeiros et al . 2022 [ 40 ] MNEPAPP peptide Tryparedoxin peroxidase LBRM.15.1100 Menezes-Souza, D. et al ., 2014 [ 42 ] EEDESKKKSCGDEGEPKVE Peptide 1 HSP83.1 LBRM_33_0340 VTEGGEDKKK Peptide 2 EVAEAPPAEAAPA Peptide 3 Menezes-Souza, D. et al ., 2015a [ 43 ] VGGGNSKNG Peptide-1 MAPK3 LBRM_10_0620 DPAEEADAP Peptide-2 …”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

A systematic review of peptide-based serological tests for the diagnosis of leishmaniasis

et al. 2023

View full text Add to dashboard Cite

Serological methods should meet the needs of leishmaniasis diagnosis due to their high sensitivity and specificity, economical and adaptable rapid diagnostic test format, and ease of use. Currently, the performances of serological diagnostic tests, despite improvements with recombinant proteins, vary greatly depending on the clinical form of leishmaniasis and the endemic area. Peptide-based serological tests are promising as they could compensate for antigenic variability and improve performance, independently of Leishmania species and subspecies circulating in the endemic areas. The objective of this systematic review was to inventory all studies published from 2002 to 2022 that evaluate synthetic peptides for serological diagnosis of human leishmaniases and also to highlight the performance (e.g., sensitivity and specificity) of each peptide reported in these studies. All clinical forms of leishmaniasis, visceral and tegumentary, and all Leishmania species responsible for these diseases were considered. Following PRISMA statement recommendations, 1,405 studies were identified but only 22 articles met the selection criteria and were included in this systematic review. These original research articles described 77 different peptides, of which several have promising performance for visceral or tegumentary leishmaniasis diagnosis. This review highlights the importance of and growing interest in synthetic peptides used for serological diagnosis of leishmaniases, and their performances compared to some widely used tests with recombinant proteins.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

A systematic review of peptide-based serological tests for the diagnosis of leishmaniasis

et al. 2023

View full text Add to dashboard Cite

show abstract

“…An ELISA evaluation of the tryparedoxin peroxidase recombinant protein, which was identified in this study, showed 100% sensitivity and specificity for both CL and ML ( 51 ). Proteins found in this immunoproteomic study, such as enolase protein, eukaryotic elongation factor-1 beta protein, eukaryotic Initiation factor 5a, tryparedoxin peroxidase, and hypothetical protein LbHyM, were later evaluated and showed high potential for the serodiagnosis of the disease or immunogenic efficacy against TL and/or even VL in a murine model ( 18 , 68 – 72 ).…”

Section: Immunoproteomics For Tegumentary Leishmaniasismentioning

confidence: 99%

Immunoproteomics and phage display in the context of leishmaniasis complexity

2023

View full text Add to dashboard Cite

Leishmaniasis is defined as a complex of diseases caused by protozoan parasites of the genus Leishmania, which comprises 20 parasite species pathogenic to mammalians, such as humans and dogs. From a clinical point of view, and considering the diversity and biological complexity of the parasites, vectors, and vertebrate hosts, leishmaniasis is classified according to the distinct clinical manifestations, such as tegumentary (involving the cutaneous, mucosal, and cutaneous-diffuse forms) and visceral leishmaniasis. Many issues and challenges remain unaddressed, which could be attributed to the complexity and diversity of the disease. The current demand for the identification of new Leishmania antigenic targets for the development of multicomponent-based vaccines, as well as for the production of specific diagnostic tests, is evident. In recent years, biotechnological tools have allowed the identification of several Leishmania biomarkers that might potentially be used for diagnosis and have an application in vaccine development. In this Mini Review, we discuss the different aspects of this complex disease that have been addressed by technologies such as immunoproteomics and phage display. It is extremely important to be aware of the potential applications of antigens selected in different screening context, so that they can be used appropriately, so understanding their performance, characteristics, and self-limitations.

show abstract

IgE Epitope Analysis and Hypo-Immunoreactivity Derivative of Arginine Kinase in Mantis Shrimp (Oratosquilla oratoria)

Chen

Yang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

As the main allergenic food, shrimp can trigger allergic reactions in various degrees. In this study, arginine kinase (AK) was identified as an allergen in Oratosquilla oratoria by LC–MS/MS. The open reading frame of AK was obtained, which included 356 amino acids, and recombinant AK (rAK) was expressed in Escherichia coli. The results of immunological analysis and circular dichroism showed that rAK displayed similar IgG-/IgE-binding activity and structure as native AK. Besides, five IgE linear epitopes of AK were verified by serological analysis, on the basis of which an epitope-deleted derivative was obtained and named as mAK-L. It has been shown that mAK-L displayed hypo-immunoreactivity compared to rAK, and the contents of secondary structures were different. In conclusion, these discoveries enrich the overall understanding of crustacean allergens and epitopes and set the foundations for food allergy diagnosis and immunotherapy.

show abstract

Mapping linear B-cell epitopes of the Tryparedoxin Peroxidase and its implications in the serological diagnosis of tegumentary leishmaniasis

Cited by 4 publications

References 64 publications

A systematic review of peptide-based serological tests for the diagnosis of leishmaniasis

A systematic review of peptide-based serological tests for the diagnosis of leishmaniasis

Immunoproteomics and phage display in the context of leishmaniasis complexity

IgE Epitope Analysis and Hypo-Immunoreactivity Derivative of Arginine Kinase in Mantis Shrimp (Oratosquilla oratoria)

Contact Info

Product

Resources

About