Abstract:As the main allergenic food, shrimp can trigger allergic
reactions
in various degrees. In this study, arginine kinase (AK) was identified
as an allergen in Oratosquilla oratoria by LC–MS/MS. The open reading frame of AK was obtained, which
included 356 amino acids, and recombinant AK (rAK) was expressed in Escherichia coli. The results of immunological analysis
and circular dichroism showed that rAK displayed similar IgG-/IgE-binding
activity and structure as native AK. Besides, five IgE linear epitopes
of AK … Show more
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