Mapping Functional Connectivity Using Cerebral Blood Flow in the Mouse Brain

Bergonzi, Karla M.; Bauer, Adam Q.; Wright, Patrick W.; Culver, Joseph P.

doi:10.1038/jcbfm.2014.211

Cited by 35 publications

(37 citation statements)

References 14 publications

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“…fm-fUS is currently limited to a single imaging plane at a time. Optical imaging technologies such as intrinsic optical imaging 22 or laser speckle imaging 23 have advantages for applications requiring large FOVs, such as resting-state functional connectivity studies. However, fm-fUS has not yet reached full maturity, and miniature two-dimensional (2D) matrix array transducers or fastscanning strategies currently under development will enable 3D imaging of the whole brain.…”

Section: Real-time Decoding Of Brain Activitymentioning

confidence: 99%

Real-time imaging of brain activity in freely moving rats using functional ultrasound

et al. 2015

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Innovative imaging methods help to investigate the complex relationship between brain activity and behavior in freely moving animals. Functional ultrasound (fUS) is an imaging modality suitable for recording cerebral blood volume (CBV) dynamics in the whole brain but has so far been used only in head-fixed and anesthetized rodents. We designed a fUS device for tethered brain imaging in freely moving rats based on a miniaturized ultrasound probe and a custom-made ultrasound scanner. We monitored CBV changes in rats during various behavioral states such as quiet rest, after whisker or visual stimulations, and in a food-reinforced operant task. We show that fUS imaging in freely moving rats could efficiently decode brain activity in real time.

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Section: Real-time Decoding Of Brain Activitymentioning

confidence: 99%

Real-time imaging of brain activity in freely moving rats using functional ultrasound

et al. 2015

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“…However, a number of previous studies using the same fcOIS system described here have established baseline FC topography in both wild-type B6 (Bero, et al, 2012) and Swiss-Webster mice (e.g. (Bauer, et al, 2014, Bergonzi, et al, 2015)). Because the scope of this study was to examine the ability of the 2D2 mouse model and fcOIS imaging system to recapitulate previously-reported ON-specific effects on FC, especially in the visual cortex (Wu et al 2015), the immunized pooled WT and 2D2 group with negligible optic nerve inflammation used here serves as an appropriate direct control.…”

Section: Discussionmentioning

confidence: 99%

“…Transcranial FC OIS (fcOIS) imaging has already been used to reveal resting state FC networks in a murine system (White, et al, 2011). Mouse fcOIS has been shown to be a sensitive assay for several neurological diseases, including ischemic stroke and Alzheimer’s disease (Bauer, et al, 2014, Bergonzi, et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Functional connectivity alterations in a murine model of optic neuritis

Wright

Archambault

Peek

et al. 2017

Experimental Neurology

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The basis for neuronal dysfunction following inflammatory demyelination of the central nervous system (CNS) remains poorly understood. We characterized the network response to white matter injury in the anterior visual pathway using an experimental model of optic neuritis (ON), as ON is often an early manifestation of immune-mediated CNS demyelination in multiple sclerosis (MS). Optical intrinsic signal imaging was performed before and after the induction of ON in mice to measure changes in cortical network functional connectivity. We observed a greater loss of connectivity between homotopic visual cortices in ON mice compared to controls. Further, decreases in homotopic visual cortex connectivity were associated with visual acuity loss in ON mice. These results demonstrate that network connectivity changes resulting from ON can be modeled in an experimental murine system. Future studies will identify the mechanisms that cause neuronal dysfunction due to white matter injury seen in MS.

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“…[22][23][24] The reconstructed images of HbO 2 , HbR, and HbT have magnitude, temporal response, and axial location that are consistent with previous functional imaging studies. 25,26 As a function of depth, the SI-DOT images show an increase in HbO 2 and decrease in HbR corresponding to somatosensory layers 2 to 4. This depth-dependent hemodynamic activity is reasonably consistent with observations from invasive thinned-skull preparations in rats using multispectral imaging, 27 laminar optical tomography, 28 and noninvasive functional MRI studies in mice.…”

Section: In Vivo Noninvasive Activationsmentioning

confidence: 99%

Structured illumination diffuse optical tomography for noninvasive functional neuroimaging in mice

et al. 2017

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Abstract. Optical intrinsic signal (OIS) imaging has been a powerful tool for capturing functional brain hemodynamics in rodents. Recent wide field-of-view implementations of OIS have provided efficient maps of functional connectivity from spontaneous brain activity in mice. However, OIS requires scalp retraction and is limited to superficial cortical tissues. Diffuse optical tomography (DOT) techniques provide noninvasive imaging, but previous DOT systems for rodent neuroimaging have been limited either by sparse spatial sampling or by slow speed. Here, we develop a DOT system with asymmetric source-detector sampling that combines the high-density spatial sampling (0.4 mm) detection of a scientific complementary metal-oxide-semiconductor camera with the rapid (2 Hz) imaging of a few (<50) structured illumination (SI) patterns. Analysis techniques are developed to take advantage of the system's flexibility and optimize trade-offs among spatial sampling, imaging speed, and signal-to-noise ratio. An effective source-detector separation for the SI patterns was developed and compared with light intensity for a quantitative assessment of data quality. The light fall-off versus effective distance was also used for in situ empirical optimization of our light model. We demonstrated the feasibility of this technique by noninvasively mapping the functional response in the somatosensory cortex of the mouse following electrical stimulation of the forepaw. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Mapping Functional Connectivity Using Cerebral Blood Flow in the Mouse Brain

Cited by 35 publications

References 14 publications

Real-time imaging of brain activity in freely moving rats using functional ultrasound

Real-time imaging of brain activity in freely moving rats using functional ultrasound

Functional connectivity alterations in a murine model of optic neuritis

Structured illumination diffuse optical tomography for noninvasive functional neuroimaging in mice

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