Real-time imaging of brain activity in freely moving rats using functional ultrasound

Urban, Alan; Dussaux, Clara; Martel, Guillaume; Brunner, Clément; Macé, Émilie; Montaldo, Gabriel

doi:10.1038/nmeth.3482

Cited by 132 publications

(124 citation statements)

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“…Very recently, real-time imaging of brain activity under visual stimulation in freely moving rats using functional ultrasound has been performed [49]. Long (7 s) or short (2 s) flashes of blue light were randomly presented to the rats in their cages.…”

Section: Discussionmentioning

confidence: 99%

Gender differences in cerebral metabolism for color processing in mice: A PET/MRI Study

et al. 2017

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IntroductionColor processing is a central component of mammalian vision. Gender-related differences of color processing revealed by non-invasive functional transcranial Doppler ultrasound suggested right hemisphere pattern for blue/yellow chromatic opponency by men, and a left hemisphere pattern by women.Materials and MethodsThe present study measured the accumulation of [18F]fluorodeoxyglucose ([18F]FDG) in mouse brain using small animal positron emission tomography and magnetic resonance imaging (PET/MRI) with statistical parametric mapping (SPM) during light stimulation with blue and yellow filters compared to darkness condition.ResultsPET revealed a reverse pattern relative to dark condition compared to previous human studies: Male mice presented with left visual cortex dominance for blue through the right eye, while female mice presented with right visual cortex dominance for blue through the left eye. We applied statistical parametric mapping (SPM) to examine gender differences in activated architectonic areas within the orbital and medial prefrontal cortex and related cortical and sub-cortical areas that lead to the striatum, medial thalamus and other brain areas. The metabolic connectivity of the orbital and medial prefrontal cortex evoked by blue stimulation spread through a wide range of brain structures implicated in viscerosensory and visceromotor systems in the left intra-hemispheric regions in male, but in the right-to-left inter-hemispheric regions in female mice. Color functional ocular dominance plasticity was noted in the right eye in male mice but in the left eye in female mice.ConclusionsThis study of color processing in an animal model could be applied in the study of the role of gender differences in brain disease.

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Section: Discussionmentioning

confidence: 99%

Gender differences in cerebral metabolism for color processing in mice: A PET/MRI Study

et al. 2017

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“…Indeed, the ultrasonic probe can be miniaturized and implanted on the rodent's head. 13 The chronic fUS recordings presented in this paper are an important first step toward this with the possibility to induce ischemia in awake rodents. Finally, it might be possible in the future to image the full 3D brain in a single acquisition by using 2D matrix transducers, and to image both CBV and CBF.…”

Section: Fus Imagingmentioning

confidence: 94%

Mapping the dynamics of brain perfusion using functional ultrasound in a rat model of transient middle cerebral artery occlusion

Brunner

Isabel

Martín

et al. 2016

J Cereb Blood Flow Metab

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Following middle cerebral artery occlusion, tissue outcome ranges from normal to infarcted depending on depth and duration of hypoperfusion as well as occurrence and efficiency of reperfusion. However, the precise time course of these changes in relation to tissue and behavioral outcome remains unsettled. To address these issues, a three-dimensional wide field-of-view and real-time quantitative functional imaging technique able to map perfusion in the rodent brain would be desirable. Here, we applied functional ultrasound imaging, a novel approach to map relative cerebral blood volume without contrast agent, in a rat model of brief proximal transient middle cerebral artery occlusion to assess perfusion in penetrating arterioles and venules acutely and over six days thanks to a thinned-skull preparation. Functional ultrasound imaging efficiently mapped the acute changes in relative cerebral blood volume during occlusion and following reperfusion with high spatial resolution (100 mm), notably documenting marked focal decreases during occlusion, and was able to chart the fine dynamics of tissue reperfusion (rate: one frame/5 s) in the individual rat. No behavioral and only mild post-mortem immunofluorescence changes were observed. Our study suggests functional ultrasound is a particularly well-adapted imaging technique to study cerebral perfusion in acute experimental stroke longitudinally from the hyper-acute up to the chronic stage in the same subject. KeywordsCerebral blood volume, functional ultrasound imaging, transient ischemic attack, transient middle cerebral artery occlusion, selective neuronal loss

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“…Recently, this technique has been proposed in a number of applications. For example, it has been used in "ultrafast" imaging 4 applications and transient elastography, 1 in color-encoded speckle imaging, 5 to characterize blood flow, 4 to analyze speckle coherence in anisotropic media using backscatter tensor imaging, 6 to obtain functional brain imaging on rodents, 7,8 and more recently in speed-of-sound estimation. 9 Through these applications, PWT-SA has been popularized as a method to achieve high frame rate synthetic transmit aperture imaging 1,10 to improve image resolution everywhere, and to improve sensitivity in Doppler imaging.…”

Section: Introductionmentioning

confidence: 99%

Angular coherence in ultrasound imaging: Theory and applications

Dahl

2017

The Journal of the Acoustical Society of America

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The popularity of plane-wave transmits at multiple transmit angles for synthetic transmit aperture (or coherent compounding) has spawned a number of adaptations and new developments of ultrasonic imaging. However, the coherence properties of backscattered signals with plane-wave transmits at different angles are unknown and may impact a subset of these techniques. To provide a framework for the analysis of the coherence properties of such signals, this article introduces the angular coherence theory in medical ultrasound imaging. The theory indicates that the correlation function of such signals forms a Fourier transform pair with autocorrelation function of the receive aperture function. This conclusion can be considered as an extended form of the van Cittert Zernike theorem. The theory is validated with simulation and experimental results obtained on speckle targets. On the basis of the angular coherence of the backscattered wave, a new short-lag angular coherence beamformer is proposed and compared with an existing spatial-coherence-based beamformer. An application of the theory in phase shift estimation and speed of sound estimation is also presented.

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Real-time imaging of brain activity in freely moving rats using functional ultrasound

Cited by 132 publications

References 27 publications

Gender differences in cerebral metabolism for color processing in mice: A PET/MRI Study

Gender differences in cerebral metabolism for color processing in mice: A PET/MRI Study

Mapping the dynamics of brain perfusion using functional ultrasound in a rat model of transient middle cerebral artery occlusion

Angular coherence in ultrasound imaging: Theory and applications

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