Mapping and validation of QTLs for resistance to aphids and whiteflies in melon

Boissot, Nathalie; Thomas, Sophie; Sauvion, Nicolas; Marchal, Cécile; Pavis, Claude; Dogimont, Catherine

doi:10.1007/s00122-010-1287-8

Cited by 63 publications

(42 citation statements)

References 44 publications

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“…Three dominant genes, Rag1, Rag2 and Rag3, for resistance to the soybean aphid (A. glycines) were mapped to independent soybean linkage groups (Hill et al 2009;Zhang et al 2010). Four quantitative trait loci (QTLs) and two pairs of epistatic QTLs for resistance to melon-cotton aphid were mapped, and the major gene vat gene was cloned (Boissot et al 2010;Pauquet et al 2004). Different species might have different inheritance models for aphid resistance.…”

Section: Discussionmentioning

confidence: 99%

Genetic inheritance analysis of melon aphid (Aphis gossypii Glover) resistance in cucumber (Cucumis sativus L.)

Liang

et al. 2015

Euphytica

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The melon aphid Aphis gossypii Glover is one of the most serious pests in cucumber production, often causing severe damage in commercial fields. Identifying and deploying resistant germplasm and understanding the inheritance of melon aphid resistance are required for cucumber geneticists to develop an effective breeding strategy. In this study, resistance of 30 cucumber selections to melon aphid was evaluated at the seedling stage. Six generations, P 1 , P 2 , F 1 , BC 1 , BC 2 and F 2 derived from the cross of JY30 (susceptible) 9 EP6392 (resistant), were used as genetic populations to study the inheritance of melon aphid resistance in cucumber with the mixed major gene plus polygene inheritance model with the joint analysis method of multiple generations. Eight of the 30 tested selections displayed resistance to the melon aphid. The resistance of cucumber to melon aphids was controlled by one additive and dominant major gene plus additive and dominant polygenes, and was affected by environment as well. The additive effect and the dominant effect of the major gene were greater than the additive effect and the dominant effect of the polygenes. The heritabilities of the major gene in BC 1 , BC 2 and F 2 were 63.62 %, 0 % and 70.39 %, respectively. The polygenic heritabilities were 22.62 %, 37.0 % and 9.32 %, and the ratios of the environmental variance to phenotype variance were 58.54 %, 63.16 % and 30.77 %. We conclude that selections of cucumber with high resistance to melon aphid could be screened in advanced generations.

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Section: Discussionmentioning

confidence: 99%

Genetic inheritance analysis of melon aphid (Aphis gossypii Glover) resistance in cucumber (Cucumis sativus L.)

Liang

et al. 2015

Euphytica

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“…Recently, part of this variability has been genetically dissected by QTL analysis [18,23-27]. Inter-population QTL comparisons among these maps are, however, difficult given the aforementioned technical barriers.…”

Section: Introductionmentioning

confidence: 99%

A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis meloL.)

et al. 2011

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BackgroundA number of molecular marker linkage maps have been developed for melon (Cucumis melo L.) over the last two decades. However, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (QTL) analysis, increasing breeding effectiveness and efficiency via marker-assisted selection (MAS).ResultsUnder the framework of the International Cucurbit Genomics Initiative (ICuGI, http://www.icugi.org), an integrated genetic map has been constructed by merging data from eight independent mapping experiments using a genetically diverse array of parental lines. The consensus map spans 1150 cM across the 12 melon linkage groups and is composed of 1592 markers (640 SSRs, 330 SNPs, 252 AFLPs, 239 RFLPs, 89 RAPDs, 15 IMAs, 16 indels and 11 morphological traits) with a mean marker density of 0.72 cM/marker. One hundred and ninety-six of these markers (157 SSRs, 32 SNPs, 6 indels and 1 RAPD) were newly developed, mapped or provided by industry representatives as released markers, including 27 SNPs and 5 indels from genes involved in the organic acid metabolism and transport, and 58 EST-SSRs. Additionally, 85 of 822 SSR markers contributed by Syngenta Seeds were included in the integrated map. In addition, 370 QTL controlling 62 traits from 18 previously reported mapping experiments using genetically diverse parental genotypes were also integrated into the consensus map. Some QTL associated with economically important traits detected in separate studies mapped to similar genomic positions. For example, independently identified QTL controlling fruit shape were mapped on similar genomic positions, suggesting that such QTL are possibly responsible for the phenotypic variability observed for this trait in a broad array of melon germplasm.ConclusionsEven though relatively unsaturated genetic maps in a diverse set of melon market types have been published, the integrated saturated map presented herein should be considered the initial reference map for melon. Most of the mapped markers contained in the reference map are polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation (e.g., integration of physical and genetic maps, colinearity analysis, map-based gene cloning, epistasis dissection, and marker-assisted selection).

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“…For aphid resistance, receptors including the Mi-1.2 gene in tomato and Vat genes in melon (Cucumis melo) protect against specific biotypes of Macrosiphum euphorbiae and Aphis gossypii, respectively (Rossi et al, 1998;Boissot et al, 2010). Currently, aphidassociated ligands that bind these receptors are unknown.…”

Section: Discussionmentioning

confidence: 99%

An Amino Acid Substitution Inhibits Specialist Herbivore Production of an Antagonist Effector and Recovers Insect-Induced Plant Defenses

et al. 2012

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Plants respond to insect herbivory through the production of biochemicals that function as either direct defenses or indirect defenses via the attraction of natural enemies. While attack by closely related insect pests can result in distinctive levels of induced plant defenses, precise biochemical mechanisms responsible for differing responses remain largely unknown. Cowpea (Vigna unguiculata) responds to Fall armyworm (Spodoptera frugiperda) herbivory through the detection of fragments of chloroplastic ATP synthase g-subunit proteins, termed inceptin-related peptides, present in larval oral secretions (OS). In contrast to generalists like Fall armyworm, OS of the legume-specializing velvetbean caterpillar (VBC; Anticarsia gemmatalis) do not elicit ethylene production and demonstrate significantly lower induced volatile emission in direct herbivory comparisons. Unlike all other Lepidoptera OS examined, which preferentially contain inceptin (Vu-In; + ICDINGVCVDA 2 ), VBC OS contain predominantly a C-terminal truncated peptide, Vu-In). Vu-In 2A is both inactive and functions as a potent naturally occurring antagonist of Vu-In-induced responses. To block antagonist production, amino acid substitutions at the C terminus were screened for differences in VBC gut proteolysis. A valine-substituted peptide (Vu-In+ ICDINGVCVDV 2 ) retaining full elicitor activity was found to accumulate in VBC OS. Compared with the native polypeptide, VBC that previously ingested 500 pmol of the valine-modified chloroplastic ATP synthase g-subunit precursor elicited significantly stronger plant responses in herbivory assays. We demonstrate that a specialist herbivore minimizes the activation of defenses by converting an elicitor into an antagonist effector and identify an amino acid substitution that recovers these induced plant defenses to a level observed with generalist herbivores.

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Mapping and validation of QTLs for resistance to aphids and whiteflies in melon

Cited by 63 publications

References 44 publications

Genetic inheritance analysis of melon aphid (Aphis gossypii Glover) resistance in cucumber (Cucumis sativus L.)

Genetic inheritance analysis of melon aphid (Aphis gossypii Glover) resistance in cucumber (Cucumis sativus L.)

A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis meloL.)

An Amino Acid Substitution Inhibits Specialist Herbivore Production of an Antagonist Effector and Recovers Insect-Induced Plant Defenses

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