Mapping and Determination of the cDNA Sequence of the Erc Gene Preferentially Expressed in Renal Cell Carcinoma in the Tsc2 Gene Mutant (Eker) Rat Model

Yamashita, Yokihiko; Yokoyama, Masayoshi; Kobayashi, Etsuko; Takai, Setsuo; Hino, Okio

doi:10.1006/bbrc.2000.3280

Cited by 49 publications

(51 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mesothelin-KO mice have been generated and are viable without obvious abnormalities [34]. An analogue of mesothelin has also been described in rats and termed ERC (expressed in renal carcinoma) [35]. Mesothelin is believed to be involved in the intercellular adhesion process and interacts with the CA 125 antigen (which is part of the MUC16 glycoprotein), an interaction that may be involved in the metastatic spread of ovarian tumour cells [36], while MPF stimulates the proliferation of megakaryocytes [37].…”

Section: Mesothelinmentioning

confidence: 99%

Clinical utility of diagnostic markers for malignant pleural mesothelioma

Grigoriu

Grigoriu²,

Chahine

et al. 2016

Monaldi Arch Chest Dis

View full text Add to dashboard Cite

Malignant mesothelioma has a very dismal prognosis with very few patients surviving one year after diagnosis. Early multimodal treatment, however, is expected to improve the outcome. Today, there is a strong need to have disease markers which could be used for screening, diagnosing, and/or monitoring tumour response to treatment. Old markers such as hyaluronic acid, various cytokeratin fragments (CYFRA 21.1, TPA) and other cancer antigens (CA 15.3, CA 125 or CA 19.9 or CEA) are not sensitive or specific enough and cannot be used in practice. More recently new molecules, such as soluble mesothelin and osteopontin, have been proposed for diagnostic purposes. Soluble mesothelin has a good specificity but has a suboptimal sensitivity being negative in all sarcomatoid and in up to one half of epithelioid mesothelioma. On the contrary osteopontin has an inadequate specificity. Combining different markers together does not lead to an improvement in diagnostic accuracy. Neither marker can be used for screening purposes, the main limitation being the very low incidence of the disease in the at-risk, asbestos exposed population. Mesothelin is also a promising marker for monitoring response to treatment but published data is still insufficient to make recommendations. There is still a strong need for research is this area both in order to discover new markers as well as to correct the positioning of each existing molecule (alone or in combination) is the evaluation of the patients with a mesothelioma.

show abstract

Section: Mesothelinmentioning

confidence: 99%

Clinical utility of diagnostic markers for malignant pleural mesothelioma

Grigoriu

Grigoriu²,

Chahine

et al. 2016

Monaldi Arch Chest Dis

View full text Add to dashboard Cite

show abstract

“…When cDNA RDA [1][2][3][4][5][6] or RDA [17][18][19] is performed, sample cDNAs or genome DNAs are digested into fragmented populations to prepare representations, and the digestion products showed as smears by agarose gel electrophoresis. Therefore, it is difficult to determine the most appropriate digestion degree.…”

Section: Discussionmentioning

confidence: 99%

“…cDNA representational difference analysis (cDNA RDA), with high specificity by eliminating those fragments present in two compared populations and leaving only the differences, has been employed with some degree of success [1][2][3][4][5][6] . While cDNA RDA, like other PCRbased difference screening methods, is prone to produce false positive results [7,8] .…”

Section: Introductionmentioning

confidence: 99%

Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells

Li¹,

Lü²

2002

WJG

View full text Add to dashboard Cite

AIM: To develop and optimize cDNA representational difference analysis (cDNA RDA) method and to identify and clone garlic up-regulated genes in human gastric cancer (HGC) cells. METHODS:We performed cDNA RDA method by using abundant double-stranded cDNA messages provided by two self-constructed cDNA libraries (Allitridi-treated and paternal HGC cell line BGC823 cells cDNA libraries respectively). BamH I and Xho I restriction sites harbored in the library vector were used to select representations. Northern and Slot blots analyses were employed to identify the obtained difference products. RESULTS:Fragments released from the cDNA library vector after restriction endonuclease digestion acted as good marker indicating the appropriate digestion degree for library DNA. Two novel expressed sequence tags (ESTs) and a recombinant gene were obtained. Slot blots result showed a 8-fold increase of gliaderived nexin/protease nexin 1 (GDN/PN1) gene expression level and 4-fold increase of hepatitis B virus x-interacting protein (XIP) mRNA level in BGC823 cells after Allitridi treatment for 72h.CONCLUSION: Elevated levels of GDN/PN1 and XIP mRNAs induced by Allitridi provide valuable molecular evidence for elucidating the garlic's efficacies against neurodegenerative and inflammatory diseases. Isolation of a recombinant gene and two novel ESTs further show cDNA RDA based on cDNA libraries to be a powerful method with high specificity and reproducibility in cloning differentially expressed genes.Li Y, Lu YY. Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells. World J Gastroenterol 2002;8(2):213-216

show abstract

“…In earlier studies, we isolated the rat Erc (Expressed in renal carcinoma) gene that was abundantly expressed in renal carcinoma tissues of the Eker rat (1,2). Mesothelin (MSLN), the human homologue of rat Erc, was identified as the antigenic target of a monoclonal antibody that reacted with a cell surface protein of malignant mesothelioma (MM) and ovarian cancer (3).…”

Section: Introductionmentioning

confidence: 99%

Suppression of cell death by the secretory form of N-terminal ERC/mesothelin

Wang

Kajino²,

Abe³

et al. 2010

Int J Mol Med

View full text Add to dashboard Cite

Abstract. ERC/mesothelin is highly expressed in malignant mesothelioma, pancreatic cancer, and ovarian cancer. It is cleaved to a 30 kDa N-terminal secretory form (N-ERC) and a 40 kDa C-terminal membranous form (C-ERC). Several functions have been reported for full-length ERC (full-ERC) and C-ERC/mesothelin, such as in cell adhesion and invasion, stimulation of cell proliferation, and the suppression of cell death. However, there have been no studies to date on the function of secretory N-ERC, despite the fact that it is abundantly secreted into the sera of mesothelioma patients. In this study, we investigated whether N-ERC could function as a secretory factor to stimulate tumor progression. Full-, N, or C-ERC was overexpressed in the human hepatocellular carcinoma cell line Huh7 that lacks endogenous expression of ERC/mesothelin. Changes in the rates of cell proliferation and cell death were determined, and the state of signal transducers was examined using various endpoints: total cell counts, trypan blue exclusion rate, BrdU incorporation rate, TUNEL assay, and the phosphorylation of ERK1/2 and Stat3. In cells overexpressing N-ERC, phosphorylation of ERK1/2 was enhanced and the rate of cell death decreased, leading to the increase of cell number. The culture medium containing the secretory N-ERC also had the activity to increase the number of cells. Our data suggested that one of the full-ERC functions reported previously was mediated by the secretory

show abstract

Mapping and Determination of the cDNA Sequence of the Erc Gene Preferentially Expressed in Renal Cell Carcinoma in the Tsc2 Gene Mutant (Eker) Rat Model

Cited by 49 publications

References 34 publications

Clinical utility of diagnostic markers for malignant pleural mesothelioma

Clinical utility of diagnostic markers for malignant pleural mesothelioma

Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells

Suppression of cell death by the secretory form of N-terminal ERC/mesothelin

Contact Info

Product

Resources

About