Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells

Li, Yong; Lü, Youyong

doi:10.3748/wjg.v8.i2.213

Cited by 10 publications

(5 citation statements)

References 52 publications

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“…Therefore, HBXIP may also contribute more besides its effects on apoptosis and cell proliferation. Additionally, the expression of HBXIP could be regulated by some factors, such as garlic [24] , gonadotropins, and forskolin [25] . Taken together, our findings indicate that one of the functions of HBXIP is its involvement in proliferation regulation in cancer cell lines and the normal liver cell line, which is related to cell-cycle transition through checkpoint controls at the G 0 /G 1 or G 2 /M phases and the downregulation of p27.…”

Section: Discussionmentioning

confidence: 99%

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Involvement of hepatitis B X-interacting protein (HBXIP) in proliferation regulation of cells

Wang

Zhang

et al. 2007

Acta Pharmacologica Sinica

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Aim: To investigat the effect of Hepatitis B X-interacting protein (HBXIP) on cell proliferation. Methods: A rabbit antibody against HBXIP was generated. The RNA interference (RNAi) fragment of the HBXIP gene was constructed in the pSilencer-3.0-H1 vector termed pSilencer-hbxip. Plasmids of the pcDNA3-hbxip encoding HBXIP gene and pSilencer-hbxip were transfected into human breast carcinoma MCF-7 cells, hepatoma H7402 cells, and the normal human hepatic cell line L-O2, respectively. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and 5-bromo-2-deoxyuridine incorporation assay were applied to detect cell proliferation. MCF-7 cells and L-O2 cells in the cell cycle were examined by flow cytometry. The proteins involved in cell proliferation and cell cycle were investigated by Western blot. Results: Overexpression of HBXIP resulted in the promotion of proliferation of MCF-7, H7402, and L-O2 cells. Flow cytometry showed that the overexpression of HBXIP promoted the cell proliferation of MCF-7 and L-O2 cells, and led to an increased cell proliferative index in MCF-7 cells (from 46.25% to 58.28%) and L-O2 cells (from 29.62% to 35.54%). Western blot showed that expression levels of c-Myc, Bcl-2, and proliferating cell nuclear antigen were upregulated in MCF-7, H7402, or L-O2 cells, whereas that of p27 was downregulated. However, the RNAi of HBXIP brought opposite results. Conclusion: One of the functions of HBXIP is its involvement in cell proliferation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Involvement of hepatitis B X-interacting protein (HBXIP) in proliferation regulation of cells

Wang

Zhang

et al. 2007

Acta Pharmacologica Sinica

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“…Furthermore, it is noteworthy that some genes such as marker genes of the cell line could play important roles, and they would have major weights in cellular function. As shown in Figure D, we specially investigated the detailed expressions of GAPDH and 36 kinds of representative genes whose transcripts had been previously measured as marker or well-expressed genes in BGC823 or other relevant gastric cancer cell lines/tissues using bulk-cell sequencing techniques such as the microarray. − All aliases used in the former works have been modified to the official symbols in the NCBI Gene Database . The data used in the heatmap were logarithm-normalized, log 2 (TPM + 1), for similarity measurement.…”

Section: Resultsmentioning

confidence: 99%

Single-Cell Multimodal Analytical Approach by Integrating Raman Optical Tweezers and RNA Sequencing

et al. 2020

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Single-cell analysis has become a state-of-art approach to heterogeneity profiling in tumor cells. Herein, we realize a kind of single-cell multimodal analytical approach by combining single-cell RNA sequencing (scRNA-seq) with Raman optical tweezers (ROT), a label-free single-cell identification and isolation technique, and apply it to investigate drug sensitivity. The drug sensitivity of human BGC823 gastric cancer cells toward different drugs, paclitaxel and sodium dichloroacetate, was distinguished in the conjoint analytical way including morphology monitoring, Raman identification, and transcriptomic profiling. Each individual BGC823 cancer cell was measured by Raman spectroscopy, then nondestructively isolated out by ROT, and finally RNA-sequenced. Our results demonstrate each analytical mode can reflect cell response to the drugs from different perspectives and is consistent and complementary with each other. Therefore, we believe the multimodal analytical approach offers an access to comprehensive characterizations of the unicellular complexity, which especially makes sense for studying tumor heterogeneity or a desired special cell from a mixture cell sample such as whole blood.

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“…Chemoprevention is always used as the main treatment for advanced cancer, and has been under intense investigation [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49] . Understanding the mechanisms through which β-ionone suppresses the proliferation of gastric adenocarcinoma cells could provide a way to prevent this disease in general population.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis of human gastric adenocarcinoma cells induced by β-ionone

Liu¹,

Chen²,

Yang³

et al. 2004

WJG

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AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901. METHODS:Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200 µmol/L) for 24 h, 48 h. RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC 50 value of β-ionone for SGC-7901 cells was estimated to be 89 µmol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-iononetreated SGC-7901 cells by the TUNEL assay.CONCLUSION: β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.

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Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells

Cited by 10 publications

References 52 publications

Involvement of hepatitis B X-interacting protein (HBXIP) in proliferation regulation of cells

Involvement of hepatitis B X-interacting protein (HBXIP) in proliferation regulation of cells

Single-Cell Multimodal Analytical Approach by Integrating Raman Optical Tweezers and RNA Sequencing

Apoptosis of human gastric adenocarcinoma cells induced by β-ionone

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