Apoptosis of human gastric adenocarcinoma cells induced by β-ionone

Abstract

AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901. METHODS:Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200 µmol/L) for 24 h, 48 h. RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC 50 value of β-ionone for SGC-7901 cells was estimated to be 89 µmol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-iononetreated SGC-7901 cells by the TUNEL assay.CONCLUSION: β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.

β‐Ionone suppresses mammary carcinogenesis, proliferative activity and induces apoptosis in the mammary gland of the Sprague‐Dawley rat

“…Apoptosis was induced in SGC-7901 cells at doses of 25-200lmol/l of b-ionone using the TUNEL assay and showed a dose-and time-dependent response. 10 Mo and Elson 12 also showed that b-ionone initiated apoptosis and, concomitantly arrested cells in the G1 phase of the cell cycle in human and murine tumor cells. In addition, b-ionone also affected the progression of metastasis in SGC-7901 cells 11 and B16 cells.…”

“…Discussion b-Ionone, a pure isoprenoid, has been shown to possess potent antiproliferative activity 9,13,17 and to induce apoptosis 10,12 in cancer cells. Liu's study 9 reported that b-ionone at doses of 25-200 lmol/l inhibited cell proliferation and DNA synthesis in human mammary cancer MCF-7 cells in a dose-dependent manner.…”

“…Physiological functions attributed to b-ionone include the inhibition of the growth of fungi 7 and the regulation of the synthesis of mevalonate-derived constituents. 8 In previous studies, b-ionone inhibited the growth of breast-, gastric-, colon-and HL-60-cancer cells in a dosedependent manner, [9][10][11][12][13] affected the MAPK pathway of MDA MB 435 cells, 14 and induced apoptosis of SGC-7901 gastric cancer cells and B16 murine tumor cells. 10,12 In addition, b-ionone could also affect metastasis in B16 and SGC-7901 cancer cell lines.…”

“…8 In previous studies, b-ionone inhibited the growth of breast-, gastric-, colon-and HL-60-cancer cells in a dosedependent manner, [9][10][11][12][13] affected the MAPK pathway of MDA MB 435 cells, 14 and induced apoptosis of SGC-7901 gastric cancer cells and B16 murine tumor cells. 10,12 In addition, b-ionone could also affect metastasis in B16 and SGC-7901 cancer cell lines. 11,12,15 Dietary studies reveal the chemopreventive 16 and antitumor 17 activities of b-ionone.…”

Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells

“…Antitumor activities of β-ionone have been demonstrated in melanoma, breast cancer and chemical-induced rat carcinogenesis [13,22,32]. β-Ionone also inhibits the growth of various human cancer cells including breast and gastric tumor cells [8,21] and murine tumor cells [26]. Its exact mechanisms are not clear, but apoptosis is one of mechanistic actions of β-ionone on SGC-7901 gastric cancer cells, B16 murine tumor cells, and other cancer cells [8,21,26].…”